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Microbe organic fertilizer containing chelated microelements and production method thereof

A technology of trace elements and organic fertilizers, applied in the direction of organic fertilizers, the preparation of organic fertilizers, the treatment of biological organic parts, etc., to achieve the effects of strong pertinence, promotion of reproduction, and high comprehensive cost performance

Active Publication Date: 2010-10-13
SHANDONG BAOYUAN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the unit cost of chelated micro-fertilizers is generally 10 times higher than that of inorganic micro-fertilizers, and finding a better cheap and easy-to-obtain chelating agent is the key to the competitiveness of chelated micro-fertilizers

Method used

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  • Microbe organic fertilizer containing chelated microelements and production method thereof
  • Microbe organic fertilizer containing chelated microelements and production method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The original strains of three strains of Trichoderma harzianum, Bacillus subtilis, and Bacillus thuringiensis were inoculated on PDA solid medium under sterile conditions, and cultured at 35°C for 2-3 days to activate the strains. Inoculate the activated bacteria in the liquid PDA medium under sterile conditions, culture at 35°C, 200r / min, shaker for 2-3 days, and wait for the OD of the bacteria 600 A value of 4.0 was used to stop the cultivation and produce first-grade seeds. According to the inoculum amount of 10% of the volume of the liquid medium, inoculate the first-grade seeds prepared in fermenters respectively, under the condition of 35°C, keep the rotation speed at 200r / min, the ventilation rate 1:1, and cultivate for 3 days to obtain the second-grade seeds. seed. According to the inoculum amount of 10% of the volume of the liquid medium, inoculate the secondary seeds in a fermenter for high-density fermentation culture. The medium is PDA, the culture temperat...

Embodiment 2

[0047] The original strains of three strains of Trichoderma harzianum, Bacillus subtilis, and Bacillus thuringiensis were inoculated on PDA solid medium under sterile conditions, and cultured at 25°C for 3 days to activate the strains. Inoculate the activated strains in the liquid PDA medium under aseptic conditions, culture at 30°C, 180r / min, on a shaking table for 3 days, and wait until the OD of the bacterial solution 600 A value of 4.0 was used to stop the cultivation and produce first-grade seeds. According to the inoculum amount of 20% of the volume of the liquid medium, inoculate the first-grade seeds prepared in fermenters respectively, under the condition of 37°C, keep the rotating speed at 300r / min, and the ventilation rate 1:1.5, and cultivate for 3 days to obtain the second-grade seeds. seed. According to the inoculum amount of 20% of the volume of the liquid medium, inoculate the secondary seeds in the fermenter to carry out high-density fermentation culture. The...

Embodiment 3

[0052] The microbial organic fertilizer containing chelated trace elements prepared by the method described in Example 1 was tested in the test field of Yantai Research Institute in Yantai City, Shandong Province. 60m each for the test area 2 , each treatment is respectively five years of continuous cropping peanut soil, no common soil for continuous cropping, 8 kg of common commercial organic fertilizers applied to the soil of five years of continuous cropping, and 8 kg of fertilizer of the present invention added to the soil of five years of continuous cropping. Each treatment was replicated three times for a total of 12 plots. The peanut variety is Luhua peanut, and the fertilization treatment is applied after sowing and emergence. Peanuts are harvested when they are ripe. The experimental results are shown in Table 1.

[0053] Table 1 The effects of microbial organic fertilizers containing chelated trace elements on peanut production and disease prevention

[0054] ...

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Abstract

The invention relates to a microbe organic fertilizer containing chelated microelements and a production method thereof. The fertilizer contains rich antagonistic microbes, i.e. trichoderma harzianum, bacillus subtilis and bacillus thuringiensis, and the chelated microelements with an extremely high chelation rate, and while preventing and treating continuous cropping obstacles and increasing the crop yield, the fertilizer can supply needed microelements to crops. The sources of the materials for the production of the fertilizer are wide and rich, the cost is low, the technique is simple, the product price is low, the fertilizer is highly specific, the market space is large, and the comprehensive cost performance is high. The yield of peanut can be increased by 69.9 percent, soil-borne diseases, such as root rot and rhizoctonia rot, can be reduced by 80.6 percent, and disease-preventing and yield-increasing effects are remarkable.

Description

technical field [0001] The invention relates to the biochemical technology of agricultural intensive production, in particular to a microbial organic fertilizer containing chelated trace elements and a production method thereof. The fertilizer is not only rich in antagonistic microorganisms, but also rich in chelated trace elements. Background technique [0002] Due to the single species of crops planted in modern intensive agriculture, the high rate of multiple cropping, and the large amount of chemical fertilizers used, the physical and chemical properties of the soil are changed, and the soil micro-ecological balance is out of balance, which in turn induces frequent cropping obstacles, seriously affecting the yield, quality and reliability of agriculture. Continuous development. In recent years, not only continuous cropping obstacles in protected cultivation have occurred continuously, but also the yield and quality of field crops such as cotton, peanuts, fruit trees, et...

Claims

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Application Information

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IPC IPC(8): C05G3/00C05F17/00
CPCY02W30/40
Inventor 赵建华臧宏伟孙磊
Owner SHANDONG BAOYUAN BIOTECH CO LTD
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