Cell culture dish for constant-volume perfusion
A technology of cell culture and constant volume, applied in the field of cell culture dishes, can solve the problems of poor effect of medium exchange, large volume, inability to quickly and fully exchange medium and save drugs, etc., to save drugs, reduce volume, and meet volume the desired effect
Inactive Publication Date: 2012-05-30
FOURTH MILITARY MEDICAL UNIVERSITY
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Problems solved by technology
At present, the disc-shaped culture dish with a diameter of 35mm commonly used for culturing cells has a large amount of liquid (about 3ml) in the dish, and the effect of changing the liquid is generally not good, and cannot achieve the effect of fast and sufficient liquid change and saving drugs.
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[0017] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.
[0018] like figure 1 , figure 2 As shown, a cell culture dish with constant volume perfusion includes an outer box 10, and an inner box 11 is set in the outer box 10. The inner box is in the shape of an inverted bamboo hat, and the two sides of the upper circular table surface are respectively arranged to the lower conical surface 8. There is a pipeline, one of which is the liquid inlet pipe 9, which is L-shaped; the other is the liquid outlet pipe 2, which is U-shaped; the inlet 12 of the liquid inlet pipe is connected to the perfusion drug delivery device; the outlet 1 of the liquid outlet pipe is connected to the negative pressure suction Device; the outlet 7 of the liquid inlet pipe on the conical surface is lower than the inlet 5 of the liquid outlet pipe on the conical surface, and the inlet 5 of the liquid outlet pipe on the ...
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Abstract
The invention discloses a cell culture dish for constant-volume perfusion, which comprises an outer box and is characterized in that an inner box is sleeved in the outer box; the inner box takes a shape of an inverted bamboo hat; channels are respectively arranged between both sides of the upper circular truncated cone surface and the lower conical surface; one channel is a liquid inlet pipe and is of an L-shape; the other channel is a liquid outlet pipe and is of a U-shape; an inlet of the liquid inlet pipe is communicated with a perfusion administration device; an outlet of the liquid outlet pipe is communicated with a negative pressure suction device; an outlet of the liquid inlet pipe on the conical surface is lower than an inlet of the liquid outlet pipe on the conical surface; and the inlet of the liquid outlet pipe on the conical surface is upward.
Description
technical field [0001] The invention relates to a medical experiment vessel, in particular to a cell culture vessel capable of constant volume perfusion drug delivery. Background technique [0002] In functional studies, perfusion administration of cultured living cells is a commonly used method in various medical experiments. The requirements for perfusion administration are convenience, simplicity, speed, and drug saving. The commonly used diameter of cultivating cells at present is that the disc-shaped culture dish of 35mm is because the liquid amount that holds in the dish is big (about 3ml), and the effect of changing liquid is generally not good, can not reach the effect of changing liquid rapidly and fully and saving medicine. Contents of the invention [0003] The object of the present invention is to provide a very convenient culture dish for perfusion, which can realize the task of sufficient, rapid and small liquid volume perfusion without any other auxiliary d...
Claims
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IPC IPC(8): C12M3/00C12M1/22
CPCC12M23/34C12M29/10C12M23/10
Inventor 赵玉峰王莉陈健康于军王跃民刘利兵刘芳娥曲萍杨芳黄小军周幸春铁茹刘楠田菲梁向艳张学策
Owner FOURTH MILITARY MEDICAL UNIVERSITY