Liquid film micro-extraction method for controlled substance in blood or urine

Abstract

The invention relates to a liquid film micro-extraction method for controlled substances in blood or urine, which belongs to the chemical field, is used for analyzing controlled substances in body fluid and overcomes the defects of complicated process, low enrichment rate, human body injuries and environmental pollution of a traditional method. A detection method of controlled substances in urine comprises the following steps of: mixing span 80, coal oil and dilute hydrochloric acid to prepare a stable emulsion liquid film, wherein the outer layer of the liquid film is an organic phase on the outer layer and the outer layer of the liquid film is a hydrochloric acid phase; adjusting a pH value of the urine to 8-9; and adding the liquid film to the urine for the liquid film micro-extraction and connecting with an AC to break the emulsion; and centrifugating and delaminating the broken emulsion liquid, wherein an aqueous solution of the inner phase in the lower layer is used for detecting the contents of the controlled substances in the urine. A detection method of amphetamine toxicants in blood comprises the following steps of: centrifugating blood in a centrifuge at a high speed; standing still to delaminate; taking out supernatant liquor on the upper layer to detect; and analyzing the rest by the same method with the analysis of the urine. The invention has the advantages of high enrichment recycle rate, little organic solvent consumption, normal temperature operation, less influence on operators and environment, repeated organic phase use and low detection cost.

Description

technical field [0001] The present invention belongs to the field of chemistry. Specifically related to the detection of controlled substances in body fluids. Background technique [0002] In the past, in criminal investigation or medical examination, when analyzing and testing methamphetamine, ketamine, morphine and other controlled drugs in body fluids including urine and blood, due to the complex composition of body fluids and low content of substances to be tested, there are many interference factors , the sensitivity of the determination method is low, and the test material can only be pretreated before analysis, and then the subsequent analysis is carried out. The usual control and analysis conditions or the use of concealed methods to eliminate interference are far from reaching the desired effect. Previously, it was more feasible to use liquid-liquid extraction or solid-phase extraction for purification and enrichment. However, some inherent defects of solid phase...

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a liquid film microextraction method for controlled drugs in blood and urine, which can be directly used for the analysis of controlled drugs in body fluids, and overcome the complex and enriched processes of existing solid-phase extraction and liquid-liquid extraction. The shortcomings of low efficiency, great harm to operators and pollution to the environment