Liquid film micro-extraction method for controlled substance in blood or urine
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A urine and drug technology, applied in the chemical field, can solve the problems of high hazards to operators, complicated process, environmental pollution, etc., and achieve the effect of reducing the impact of operators and the environment, reducing testing costs, and less volatilization
Inactive Publication Date: 2010-12-08
吉林省公安厅物证鉴定中心
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[0003] The purpose of the present invention is to provide a liquid film microextraction method for controlled drugs in blood and urine, which can be directly used for the analysis of controlled drugs in body flu
Method used
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Embodiment 1
[0020] Detection of toxic substances in urine:
[0021] (1) Mix span 80, kerosene, and 1% dilute hydrochloric acid in a volume ratio of 4:100:100, add the resulting mixture to a container, and stir with a high-speed mixer for 30 minutes to obtain an organic phase in the outer layer , the inner layer is a stable emulsion film of hydrochloric acid phase. Wherein the 1% concentration of hydrochloric acid is the mass ratio concentration.
[0022] (2) Adjust the pH value of the urine to be tested to 8-9 with sodium hydroxide solution, so that the controlled drugs in it are converted from ionic state to free state.
[0023] (3) Add the liquid film obtained in step 1 into the urine obtained in step 2 to carry out liquid film micro-extraction, the amount of liquid film added is 1 / 10 of the urine volume, and gently stirred for 10 minutes.
[0024] (4) Leave the milk obtained in step 3 to separate layers.
[0025] (5) Take out the upper layer emulsion formed in step 4, discard the lo...
Embodiment 2
[0028] Toxicology testing in blood:
[0029] (1) Mix span 80, kerosene, and 1% dilute hydrochloric acid in a volume ratio of 4:100:100, add the resulting mixture to a container, and stir with a high-speed mixer for 30 minutes to obtain an organic phase in the outer layer , the inner layer is a stable emulsion film of hydrochloric acid phase. Wherein the 1% concentration of hydrochloric acid is the mass ratio concentration.
[0030] (2) Use a centrifuge to centrifuge the blood to be tested at a high speed of 10,000 rpm for 5 minutes, and take out the supernatant after static layering.
[0031] (3) Add the liquid film obtained in step 1 into the clear liquid obtained in step 2 to carry out liquid film microextraction, the amount of liquid film added is 1 / 5 of the volume of the clear liquid, and gently stirred for 10 minutes.
[0032] (4) Leave the milk obtained in step 3 to separate layers.
[0033] (5) The upper emulsion formed in step 4 is taken out, the lower layer is disc...
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Abstract
The invention relates to a liquid film micro-extraction method for controlled substances in blood or urine, which belongs to the chemical field, is used for analyzing controlled substances in body fluid and overcomes the defects of complicated process, low enrichment rate, human body injuries and environmental pollution of a traditional method. A detection method of controlled substances in urine comprises the following steps of: mixing span 80, coal oil and dilute hydrochloric acid to prepare a stable emulsion liquid film, wherein the outer layer of the liquid film is an organic phase on the outer layer and the outer layer of the liquid film is a hydrochloric acid phase; adjusting a pH value of the urine to 8-9; and adding the liquid film to the urine for the liquid film micro-extraction and connecting with an AC to break the emulsion; and centrifugating and delaminating the broken emulsion liquid, wherein an aqueous solution of the inner phase in the lower layer is used for detecting the contents of the controlled substances in the urine. A detection method of amphetamine toxicants in blood comprises the following steps of: centrifugating blood in a centrifuge at a high speed; standing still to delaminate; taking out supernatant liquor on the upper layer to detect; and analyzing the rest by the same method with the analysis of the urine. The invention has the advantages of high enrichment recycle rate, little organic solvent consumption, normal temperature operation, less influence on operators and environment, repeated organic phase use and low detection cost.
Description
technical field [0001] The present invention belongs to the field of chemistry. Specifically related to the detection of controlled substances in body fluids. Background technique [0002] In the past, in criminal investigation or medical examination, when analyzing and testing methamphetamine, ketamine, morphine and other controlled drugs in body fluids including urine and blood, due to the complex composition of body fluids and low content of substances to be tested, there are many interference factors , the sensitivity of the determination method is low, and the test material can only be pretreated before analysis, and then the subsequent analysis is carried out. The usual control and analysis conditions or the use of concealed methods to eliminate interference are far from reaching the desired effect. Previously, it was more feasible to use liquid-liquid extraction or solid-phase extraction for purification and enrichment. However, some inherent defects of solid phase...
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