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Legionella transport and enrichment medium

An enrichment medium and technology of Legionella, applied in bacteria, microorganism-based methods, microorganisms, etc., can solve the problems of difficulty in isolation and culture, low positive rate of Legionella isolation and culture, etc. The positive rate of isolation and culture and the effect of reducing the inhibitory effect

Active Publication Date: 2010-12-22
重庆金域医学检验所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to overcome the difficulty in the isolation and culture of Legionella from clinical specimens. In the process of specimen collection and transportation, due to the growth of miscellaneous bacteria, Legionella is inhibited, resulting in a low positive rate of Legionella isolation and culture. A current method is provided. There is no Legionella transport enrichment medium at home and abroad

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Legionella transports the enrichment culture medium, and the component parts by weight that it comprises are as follows:

[0036] Base fluid:

[0037] yeast powder 6,

[0038] ACES 6,

[0039] a-ketoglutarate 0.5,

[0040] Iron pyrophosphate 0.2,

[0041] Amino acid protein solution:

[0042] Glycine 2.0,

[0043] L-cysteine ​​0.3,

[0044] Albumin 0.05,

[0045] Antibiotic solution:

[0046] Vancomycin 0.0005,

[0047] Polymyxin B 0.005,

[0048] Cycloheximide 0.005.

Embodiment 2

[0050] Legionella transports the enrichment culture medium, and the component parts by weight that it comprises are as follows:

[0051] Base fluid:

[0052] yeast powder 15,

[0053] ACES 15,

[0054] a-ketoglutarate 1.5,

[0055] Iron pyrophosphate 0.3,

[0056] Amino acid protein solution:

[0057] Glycine 4.0,

[0058] L-cysteine ​​0.7,

[0059] Albumin 0.15,

[0060] Antibiotic solution:

[0061] Vancomycin 0.0015,

[0062] Polymyxin B 0.012,

[0063] Cycloheximide 0.012.

Embodiment 3

[0065] Legionella transports the enrichment culture medium, and the component parts by weight that it comprises are as follows:

[0066] Base fluid:

[0067] Yeast powder 8~12,

[0068] ACES 8-12,

[0069] a-ketoglutarate 0.8~1.2,

[0070] Iron pyrophosphate 0.24~0.28,

[0071] Amino acid protein solution:

[0072] Glycine 2.5~3.5,

[0073] L-cysteine ​​0.4~0.6,

[0074] Albumin 0.08~0.12,

[0075] Antibiotic solution:

[0076] Vancomycin 0.0008~0.0012,

[0077] Polymyxin B 0.008~0.010,

[0078] Cycloheximide 0.008~0.010.

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PUM

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Abstract

The invention discloses a legionella transport and enrichment medium, which is characterized in that legionella selection components are added in the process of preparing a legionella BCYE alpha-based medium: glycin, vancomycin, polymyxin B and cycloheximide. Antibiotic at a specific optimization amount is added, which can be used for not only transporting legionella isolation culture specimens but also enriching the legionella in the transport process. The legionella transport and enrichment medium has the beneficial effects of 1, improving the inhibiting capacity to non-legionella and otherbacteria and reducing the pollution of other bacteria; and 2, reducing the inhibition to the legionella, thus improving the positive rate of legionella isolation culture.

Description

Technical field: [0001] The invention relates to a culture medium, specifically a culture medium suitable for the isolation and cultivation of Legionella in clinical specimens, which can reduce the contamination of bacteria in the process of specimen collection and transportation, but can maintain the proper growth of Legionella, thereby improving the positive isolation and culture of Legionella Rate of Legionella transport enrichment medium. Background technique: [0002] According to the international standard ISO11731:1998 for the detection of Legionella, the US Centers for Disease Control and Prevention (CDC) and the health industry standard of the Ministry of Health of my country "Diagnostic Standards and Treatment Principles for Legionnaires' Disease" WS-195-2001, the diagnosis of Legionnaires' disease, bacterial culture is the gold standard. However, the isolation and culture of Legionella requires a nutrient-rich selective isolation medium, which is technically diffi...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/01
Inventor 朱庆义胡朝晖赵利伟王娟刘洋敏
Owner 重庆金域医学检验所有限公司
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