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Mixing agent for preventing and treating wheat scab as well as preparation method and application thereof

A technology of wheat scab and mixed agent, which is applied in the direction of botany equipment and methods, application, biocide, etc., can solve the problems of chemical agent side effects, instability, long use time, etc., and achieve the effect of high inhibition ability

Pending Publication Date: 2022-04-15
INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the prevention and control methods of wheat scab mainly focus on chemical control, starting from the selection of disease-resistant varieties, and obtaining varieties resistant to wheat scab as much as possible, but this method takes a long time, is unstable, and the use of chemical agents is too Play side effects, therefore, biological control methods gradually come into people's sight

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Test strains

[0030] (1) Wheat scab (Fusarium graminearum) was purchased from Shanghai Fusheng Industrial Co., Ltd.;

[0031] (2) Bacillus cereus ML19, preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: M2014445.

[0032] (3) Medium used:

[0033] LB solid medium: tryptone 10g, yeast extract 5g, sodium chloride 10g, agar 18g, deionized water 1000mL.

[0034] Broth medium: peptone 10g, beef extract 5g, NaCl 5g, deionized water 1000mL, pH7.0.

[0035] Comprehensive PDA medium: potato 200g, glucose 20g, KH 2 PO 4 3g, MgSO 4 .7H 2 O1.5g, thiamine trace, agar 15g, distilled water 1000mL.

[0036] 2. Preparation of mixture for preventing and treating wheat scab

[0037] S1, preparation of cactus extract: Take fresh cactus and mash it, add ethanol with a concentration of 80% according to the ratio of material to liquid 1g:20mL, and extract it ultrasonically for 20min under the conditions of supersonic power 450W, temper...

Embodiment 2

[0041] This embodiment is basically the same as Embodiment 1, the difference is:

[0042] 2. During the preparation of the mixture for preventing and treating wheat scab:

[0043] S1, preparation of cactus extract: Take fresh cactus and mash it, add ethanol with a concentration of 80% according to the ratio of material to liquid 1g:23mL, and extract it by ultrasonic extraction for 25min under the conditions of supersonic power 450W, temperature 60°C, and pH 8.0, and then Centrifuge at 4500rpm for 10min, take the supernatant, concentrate under reduced pressure at 60°C, and freeze-dry in vacuum at -20°C, 10Pa for 8h to obtain the cactus extract;

[0044] S2, preparation of Bacillus cereus bacterial suspension: First, activate Bacillus cereus ML19 by streaking on LB solid medium plate, then pick a single colony and inoculate it in fresh broth medium, at 30°C, 150r / m Cultivate for 24 hours at 1 min to obtain seed liquid, transfer the seed liquid to a new broth medium according to...

Embodiment 3

[0047] This embodiment is basically the same as Embodiment 1, the difference is:

[0048] 2. During the preparation of the mixture for preventing and treating wheat scab:

[0049] S1, preparation of cactus extract: Take fresh cactus and mash it, add ethanol with a concentration of 80% according to the ratio of material to liquid 1g:26mL, and extract it by ultrasonic for 25min under the conditions of supersonic power 450W, temperature 65°C, and pH 8.0, and then Centrifuge at 4000rpm for 15min, take the supernatant, concentrate under reduced pressure at 60°C, freeze-dry in vacuum at -30°C, 6Pa for 6h, and obtain the cactus extract;

[0050]S2, preparation of Bacillus cereus bacterial suspension: First, activate Bacillus cereus ML19 by streaking on LB solid medium plate, then pick a single colony and inoculate it in fresh broth medium, at 32°C, 150r / m Cultivate for 24 hours at 1 min to obtain seed liquid, transfer the seed liquid to a new broth medium according to the inoculum s...

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Abstract

The invention relates to the field of wheat disease prevention and treatment methods, and particularly discloses a mixing agent for preventing and treating wheat scab and a preparation method and application thereof.The mixing agent is formed by mixing cactus extract and bacillus cereus suspension according to the dosage ratio of 3-5 g: 10 mL, and the effective viable count of the bacillus cereus suspension is 5 * 10 < 6 >-5 * 10 < 7 > cfu / mL. The prepared mixture can inhibit growth of fusarium graminearum, so that prevention and treatment of wheat scab are realized.

Description

technical field [0001] The invention relates to the field of methods for preventing and controlling wheat diseases, in particular to a mixture for preventing and controlling wheat scab, a preparation method and application thereof. Background technique [0002] Wheat head blight, also known as ear rot, wheat straw withered, rotten wheat head, red wheat head, and red head miasma, is a disease that occurs on wheat caused by a variety of Fusarium infection. It can occur from the seedling stage to the ear stage, causing seedling rot, stem base rot, stalk rot and ear rot, and ear rot is the most harmful. After the wheat is infected by the disease, the grain weight decreases, the germination rate decreases, the germination potential is weakened, the flour extraction rate is low, and the flour quality is poor. Thereby seriously affecting its production and economic benefits. [0003] Wheat head blight is often prevalent in winter wheat areas in southern China, such as the middle ...

Claims

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Application Information

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IPC IPC(8): A01N65/08A01N63/22A01P3/00
Inventor 夏先全叶慧丽魏会廷肖万婷罗江陶邓清燕郑建敏蒲宗君
Owner INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
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