Mixing agent for preventing and treating wheat scab as well as preparation method and application thereof

A technology of wheat scab and mixed agent, which is applied in the direction of botany equipment and methods, application, biocide, etc., can solve the problems of chemical agent side effects, instability, long use time, etc., and achieve the effect of high inhibition ability

Pending Publication Date: 2022-04-15
INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the prevention and control methods of wheat scab mainly focus on chemical control, starting from the selection of disease-resistant varieties, and obtaining varieties resistant to wheat

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0028] Example 1

[0029] 1, the test strain

[0030] (1) Wheat Bibi Chemole (Hegu Summy) Buy Since Shanghai Yuheng Industrial Co., Ltd.

[0031] (2) Wax-shaped Bacillus ML19, is prepared in the China Typical Culture Savings Savings Center, and the number is included in CCTCC NO: M2014445.

[0032] (3) Mediated medium:

[0033] LB solid medium: trypsin 10g, yeast extract 5g, sodium chloride 10g, agar 18g, deionized water 1000ml.

[0034] Snorned Soup medium: protein 10g, beef paste 5g, NaCl 5g, deionized water 1000ml, pH 7.0.

[0035] Integrated PDA medium: potato 200g, glucose 20g, kH 2 PO 4 3G, MGSO 4 .7h 2 O1.5g, tepidin, agar 15g, and 1000 ml of distilled water.

[0036] 2. Preparation of a mixture of wheat rediosis

[0037] S1, cactus extract prepare: Take fresh cactus mart, according to 1g: 20 mL of ethanol, at a super-cyclic power of 450W, temperature 60 ° C, pH 8.0, ultrasound extraction 20 min, then At 4000 rpm, centrifugation was centrifuged, and the supernatant was remo...

Example Embodiment

[0040] Example 2

[0041] The present embodiment is basically the same as that of Example 1, and the difference is:

[0042] 2. Preparation of the mixture of wheat rediacuria:

[0043] S1, cactus extract prepare: Take fresh cactus mashed, according to 1g: 23 mL, add concentration of 80% ethanol, at a super-blood power of 450W, temperature 60 ° C, pH 8.0, ultrasound extraction 25 min, then At 4500 rpm, centrifuged 10min, taking the supernatant, concentrated under reduced pressure at 60 ° C, at -20 ° C, 10Pa, vacuum freezing for 8 h, and obtained a cactus extract;

[0044] S2, Waxed Bacillus suspension Preparation: First, activate wax-shaped Bacillus ML19 on the LB solid medium plate, and then pick a single bacterius inoculated in fresh broth culture medium, at 30 ° C, 150R / The seed liquid was cultured under MIN obtained seed fluid, and the seed fluid was transferred in a new broth culture medium according to a vaccination amount (4 ml / 100 mL), and 48 h was cultured under 30 ° C...

Example Embodiment

[0046] Example 3

[0047] The present embodiment is basically the same as that of Example 1, and the difference is:

[0048] 2. Preparation of the mixture of wheat rediacuria:

[0049] S1, the cactus extract prepares: Take fresh cactus mashed, according to 1G: 26 mL of ethanol, at a super-life power of 450W, temperature 65 ° C, pH 8.0, ultrasonic extraction 25min, then At 4000 rpm, centrifugation is 15 min, the supernatant is removed, concentrated under reduced pressure at 60 ° C, and drying at -30 ° C, 6Pa, vacuum freeze 6h, and obtains a cactus extract;

[0050]S2, Cybergus suspension Preparation: First, activate wax-shaped Bacillus ML19 on the LB solid medium plate, and then pick a single bacterius in seed in fresh broth culture medium, at 32 ° C, 150R / The seed liquid was cultured under MIN to obtain a seed liquid, and the seed fluid was transferred in a new broth culture medium in a new broth culture medium in a vaccinating amount, and 48 h was cultured at 32 ° C, 150 R / mi...

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Abstract

The invention relates to the field of wheat disease prevention and treatment methods, and particularly discloses a mixing agent for preventing and treating wheat scab and a preparation method and application thereof.The mixing agent is formed by mixing cactus extract and bacillus cereus suspension according to the dosage ratio of 3-5 g: 10 mL, and the effective viable count of the bacillus cereus suspension is 5 * 10 < 6 >-5 * 10 < 7 > cfu/mL. The prepared mixture can inhibit growth of fusarium graminearum, so that prevention and treatment of wheat scab are realized.

Description

technical field [0001] The invention relates to the field of methods for preventing and controlling wheat diseases, in particular to a mixture for preventing and controlling wheat scab, a preparation method and application thereof. Background technique [0002] Wheat head blight, also known as ear rot, wheat straw withered, rotten wheat head, red wheat head, and red head miasma, is a disease that occurs on wheat caused by a variety of Fusarium infection. It can occur from the seedling stage to the ear stage, causing seedling rot, stem base rot, stalk rot and ear rot, and ear rot is the most harmful. After the wheat is infected by the disease, the grain weight decreases, the germination rate decreases, the germination potential is weakened, the flour extraction rate is low, and the flour quality is poor. Thereby seriously affecting its production and economic benefits. [0003] Wheat head blight is often prevalent in winter wheat areas in southern China, such as the middle ...

Claims

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Application Information

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IPC IPC(8): A01N65/08A01N63/22A01P3/00
Inventor 夏先全叶慧丽魏会廷肖万婷罗江陶邓清燕郑建敏蒲宗君
Owner INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
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