Progesterone detection kit of dairy cow milk

Abstract

The invention discloses a progesterone detection kit of dairy cow milk, relating to biological technologies, in particular to an immunoassay technology for enzyme labeling. A direct-competition quantitative ELISA (Enzyme-Linked Immunosorbent Assay) method is established for detecting the level of progesterone in dairy cow milk. An anti-progesterone monoclonal antibody and a progesterone or ELISA progesterone compound is indirectly fixed on a solid-phase ELISA plate by applying the ELISA method through combining the specificities of the antigen and the antibody, and the direct-competition ELISA method is established to quantitatively detect the progesterone in the dairy cow milk. The ELISA plate is coated with the anti-progesterone monoclonal antibody and the progesterone antigen is labeled with horseradish peroxidase. The invention has the advantages of simpleness, convenience, rapidness, high sensitivity, and the like and has favorable development use values when used for detecting the progesterone in the dairy cow milk.

Description

technical field The invention relates to a dairy cow milk progesterone detection kit, which is used for detecting the milk cow milk progesterone level. technical background At present, the breeding scale of dairy cows in our country is constantly expanding, and it has now become a pillar industry of animal husbandry. In order to shorten the calving interval of dairy cows, improve the utilization rate of dairy cows, and enable dairy cows to produce more milk and breed more calves, it is necessary to regularly monitor the cows after calving. Empty lactating cows are tested for ovarian progesterone. At present, rectal examination and blood sampling are commonly used for ovarian progesterone examination in the breeding field. Injury and hemorrhage will cause damage to the ovaries of dairy cows, which requires a high level of direct inspection by inspectors, but the inspection results are not very accurate; the blood collection inspection method must keep the cows in place, which...

AI Technical Summary

Problems solved by technology

At present, the breeding scale of dairy cows in our country is constantly expanding, and it has now become a pillar industry of animal husbandry. In order to shorten the calving interval of dairy cows, improve the utilization rate of dairy cows, and enable dairy cows to produce more milk and breed more calves, it is necessary to regularly monitor the cows after calving. Empty lactating cows are tested for ovarian progesterone. At present, rectal examination and blood sampling are commonly used for ovarian progesterone examination in the breeding field. Injury and hemorrhage will cause damage to the ovaries of dairy cows, which requires a high level of direct inspection by inspectors, but the inspection results are not very accurate; the blood collection inspection method must keep the cows in place, which can easily cause stress and hinder the health of dairy cows. Plasma progesterone levels synchronized

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