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Breeding method for TPS male sterile line of green vegetable

A technology of male sterile lines and green vegetables, which is applied in the field of breeding of green vegetables, can solve the problems of lack of chlorosis and yellowing of leaves, and has not been promoted to production, and achieves wide application prospects, high reproduction and seed production yields, and high use value Effect

Inactive Publication Date: 2010-12-29
上海农业科技种子有限公司
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  • Application Information

AI Technical Summary

Problems solved by technology

Male sterile plants have been found in radish in Japan, but there are bad traits such as lack of chlorosis and yellowing of leaves at low temperature in the seedling stage in the transformation of green vegetables
The above-mentioned methods have all been applied to vegetable breeding, but have not been extended to production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The breeding process of the extra dwarf green cabbage male sterile line is as follows:

[0039] (1), using Brassica napus TPS male sterile line as the female parent;

[0040] (2), using the line isolated by selfing of the extra-dwarf green cabbage variety as the male parent and the recurrent parent;

[0041] (3), the male parent and the Brassica napus TPS male sterile line are interspecifically crossed to obtain F1 generation hybrids;

[0042] (4) The reincarnation parent of the super short green is propagated and purified by selfing;

[0043] (5) The selected plants from the F1 generation were continuously backcrossed with the recurrent parent of the ultra-dwarf green cabbage for 4-5 generations to obtain a stable TPS male sterile line of the ultra-dwarf green cabbage.

Embodiment 2

[0045] (1), using the extra dwarf green cabbage TPS male sterile line as the female parent;

[0046] (2), taking the reincarnation parent of the special dwarf green variety as the male parent;

[0047] (3), the male parent is crossed with the TPS sterile line of Teihuaqing to obtain the F1 generation hybrid of Teihuaqing hybrid;

[0048] (4) The comprehensive performance of the F1 generation of Teihuaqing is dwarf, green, flat leaves, short stalk, dwarf Osmanthus, beautiful and tidy, high growth and uniformity, yield increased by 12.5% ​​compared with the male parent, and the heterosis is obvious.

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PUM

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Abstract

The invention provides a breeding method for a TPS male sterile line of green vegetable, which is characterized by fully utilizing a cabbage type male sterile line system, transferring sterile cytoplasm from the cabbage type rape to the green vegetable by crossing and backcrossing nucleus substitution, thereby culturing a superior green vegetable TPS male sterile line. The male sterile line can be used for breeding new hybrid species of the green vegetables, and has the advantages of low cost, good purity, high yield, obvious heterosis and the like. The invention fully utilizes the characteristic of stable fertility of the male sterile line of green vegetable, ensures the quality of first filial generation of seeds, is a new way for utilizing the heterosis of the green vegetable, provides a new technology for breeding vegetables, and enriches the resource pool for developing source agriculture.

Description

Technical field: [0001] The technical field relates to a method for breeding vegetables, in particular to a method for breeding TPS male sterile lines of vegetables. Background technique: [0002] At present, large-scale research on heterosis is being carried out on various crops all over the world. Compared with conventional species, the utilization of hybrid vigor and the comprehensive promotion of hybrid varieties have made great contributions to the improvement of crop yield, quality improvement, and variety variety in my country. made a huge contribution. [0003] Green vegetables are one of the main vegetable crops in Shanghai and all over the country. In Shanghai, green vegetables account for about 30% of the entire vegetable planting area and nearly 40% of the total vegetable consumption of citizens. However, most of the green vegetable varieties currently used in production are A conventional variety bred in the 1970s and 1980s. At present, hybrid vegetable varieti...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
Inventor 周志疆
Owner 上海农业科技种子有限公司
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