Application of puerarin in preparation of drugs for treating P2X3 receptor-mediated acute pain

Abstract

The invention relates to a new purpose of puerarin in the pharmaceutical field, that is an application of the puerarin in the preparation of drugs for treating P2X3 receptor-mediated acute pain. Experiments observe that the puerarin can inhibit pain behavior reaction, the further applications of the technologies of immunohistochemistry, in-situ hybridization, RT-PCR and Western blotting and the like observe that the puerarin can inhibit a dorsal root ganglion of a neuropathic pain model rat, the dorsal root ganglion of a burn model and mRNA and protein expression of a sensory nerve ending P2X3 receptor, and the use of the whole-cell patch clamp technology observes that the puerarin can obviously reduce the situation that a P2X3 receptor agonist of dorsal root ganglion neurons of the neuropathic pain model rat activates current. The experiments prove that the mechanism of inhibiting the acute pain and chronic pain of the puerarin is to block P2X3 receptor-mediated pain information transfer of primary sensory neurons. The invention discloses a new method for preventing and treating the acute pain and the chronic pain, simultaneously shows that the puerarin has the roles of a P2X3 receptor antagonist, and is conductive to applications in the drugs for preventing and treating diseases which are related to the P2X3 receptor of the nervous system.

Description

technical field [0001] The present invention relates to the field of the invention of analgesic medicine application, especially relates to puerarin which can be used to prevent and treat pain in the preparation and treatment of P2X 3 Drug use in receptor-mediated acute pain. Background technique [0002] Pain is a common symptom of most diseases, and it is an unpleasant feeling common to all human beings with great individual differences. Because pain causes great suffering to people, according to the second issue of 2001 "International Pain Society Newsletter" report: the 106th Congress of the United States passed a resolution, announcing that the ten years from January 1, 2001 will be "pain control and Decade of Pain Control and Research. This shows that countries attach importance to pain research. According to the pain time course, pain can be divided into acute pain (physiological pain) and chronic pain (pathological pain). Among them: chronic pain, including infla...

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Problems solved by technology

This literature suggests that puerarin extracted from puerarin may have the effect of antinociceptive response and affecting pain perception, but there is no report that puerarin directly has analgesic effect, and there is no clinical report that puerarin can treat pain through an analgesic mechanism report

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