Application of puerarin to preparing medicine for treating P2X3 receptor mediated nerve system disease

Abstract

The invention relates to novel application of puerarin to the pharmacy field, i.e. application of puerarin to preparing a medicine for treating a P2X3 receptor mediated nerve system disease. Experiments show that the puerarin can inhibit algesthesia behavior reaction; observed from technologies of applying immunohistochemistry, in-situ hybridization, RT-PCR, Western blotting and the like, the puerarin can inhibit nerve pathological pain model rat dorsal root ganglion, burn model rat dorsal root ganglion, sensory nerve ending P2X3 receptor mRNA and protein expression; and observed from whole cell patch clamp technologies, the puerarin can remarkably reduce activating current of a neuron P2X3 receptor stimulant of the pathological pain model rat dorsal root ganglion. Experiments show that the puerarin has the inhibition function on acute and chronic pains for inhibiting the primary sensory neuron P2X3 receptor mediated algesthesia information transfer. The invention verifies a new method for controlling the acute and the chronic pains, presents the puerarin has the function of a P2X3 receptor antagonist, and contributes to the medicine control application of the P2X3 receptor related to the nerve system disease.

Description

technical field [0001] The present invention relates to the field of the invention of analgesic medicine application, especially relates to puerarin which can be used to prevent and treat pain in the preparation and treatment of P2X 3 Receptor-mediated drug application in neurological diseases. Background technique [0002] Pain is a common symptom of most diseases, and it is an unpleasant feeling common to all human beings with great individual differences. Because pain causes great suffering to people, according to the second issue of 2001 "International Pain Society Newsletter" report: the 106th Congress of the United States passed a resolution, announcing that the ten years from January 1, 2001 will be "pain control and Decade of Pain Control and Research. This shows that countries attach importance to pain research. According to the pain time course, pain can be divided into acute pain (physiological pain) and chronic pain (pathological pain). Among them: chronic pa...

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Problems solved by technology

This literature suggests that puerarin extracted from puerarin may have the effect of antinociceptive response and affecting pain perception, but there is no report that puerarin directly has analgesic effect, and there is no clinical report that puerarin can treat pain through an analgesic mechanism report

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