Pyrene degrading bacterium and application thereof

A technology for pyrene degrading bacteria and strains, applied in the field of biodegradation treatment of contaminated soil and water, can solve the problems of restricting development and application, lack of efficient polycyclic aromatic hydrocarbon degrading bacteria and the like

Inactive Publication Date: 2011-02-16
BEIJING NORMAL UNIVERSITY
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Problems solved by technology

[0003]However, due to the lack of efficient PAH-degrading bacteria, the further develo

Method used

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Embodiment Construction

[0019] Adding petroleum-contaminated soil to the open glass bottle with pyrene and basal medium for enrichment and domestication of pyrene-degrading microorganisms. Insert the enriched acclimatized soil into the shaped bottle. After culturing in an oscillator at a temperature of 25-30°C and a rotation speed of 100r / min for 5-7 days, the cycle transfer is carried out, and after the number of cycles is more than 8 times, a degrading bacterial group that can efficiently remove pyrene can be obtained. Make the bottom plate of agar solid medium with pyrene as the only carbon source and energy source in the ultra-clean workbench, make the top plate of agar solid medium containing pyrene-degrading bacteria on the bottom plate, and place the prepared medium plate in the incubator Cultured in medium for 10-15 days. In the ultra-clean workbench, insert the colony on the medium plate into the conical flask with pyrene, basal medium, trace metal liquid, vitamin C solution and adsorbent, ...

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Abstract

The invention discloses a pyrene degrading bacterium and application thereof, and belongs to the technical field of biological degradation treatment. The pyrene degrading bacterium is authenticated as an achromobacter (Achromobacter sp.), and the strain collection number is CGMCC3637. The Genbank accession number of a 16S rDNA of the strain is EU220009.1. The strain is prepared into a bacterial suspension of which the bacterium content is 1.25*10<8>CFU/ml, and the degradation removal rates to the pyrenes of which the initial concentrations are 0.11mg/L, 0.26mg/L, 0.52mg/L and 1.14mg/L respectively after 7 days are 92.65%, 91.46%, 29% and 89.67% respectively.

Description

technical field [0001] The invention belongs to the technical field of biodegradation treatment of polluted soil and water, in particular to a pyrene-degrading bacterium and its application. Background technique [0002] Polycyclic aromatic hydrocarbons (Polycyclic Aromatic Hydrocarbons, PAHs) are a class of condensed ring compounds containing two or more benzene rings arranged in linear, angular or clustered shapes, with hydrophobicity, low solubility, low vapor pressure, High melting point and boiling point, high octanol-water partition coefficient and other characteristics. Polycyclic aromatic hydrocarbons have strong carcinogenic, teratogenic and mutagenic effects. Generally speaking, low-molecular-weight PAHs with 2-3 rings have significant acute toxicity, while some high-molecular-weight PAHs have potential carcinogenicity. Due to the presence of conjugated large π-bond electron systems in PAHs molecules, they have high stability, their photolysis and biodegradation a...

Claims

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Application Information

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IPC IPC(8): C02F3/02C12R1/025A62D101/20C02F101/32B09C1/10C12N1/20A62D3/02C02F3/34
CPCY02W10/10
Inventor 豆俊峰杜勇超丁爱中程莉蓉范福强陈海英
Owner BEIJING NORMAL UNIVERSITY
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