Complex enzyme formula for improving extraction rate of sodium alginate and application

A sodium alginate and extraction rate technology, applied in the direction of enzymes, lyases, hydrolases, etc., can solve the problems of difficult to crack cell walls and achieve the effect of easy release

Inactive Publication Date: 2012-05-23
SHANDONG GANGYUAN MARINE BIOENG CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Extracting sodium alginate by enzymatic hydrolysis extraction is not mature enough at present, only a small amount of literature reports, such as "the research of cellulase extracting sodium alginate" such as Ma Chenghao (Guangzhou Food Industry Science and Technology, 2004, 20 (4): 12 ) article introduces single pretreatment with cellulase, and then extracts sodium alginate with traditional methods, which improves the extraction rate by 21.13% compared with single traditional process; , 35(12): 3661) establish

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  • Complex enzyme formula for improving extraction rate of sodium alginate and application

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Example Embodiment

[0018] 2. The preparation method of the present invention is as follows: the raw enzymes are respectively subjected to pretreatment, and then fed into an automatic mixer according to the proportion for compounding, and then the enzyme activity is measured and quantitatively packaged to become a product.

[0019] 3. The method of use of the present invention: kelp or sargassum is removed from impurities, crushed, and then mixed with a compound enzyme. Stir evenly, add at a ratio of 5,000 to 5,000, and enzymatically hydrolyze at room temperature and natural pH. After 6h, after enzymolysis, it is extracted according to normal process.

[0020] 4. Process conditions of the present invention:

[0021] Action temperature: 15-60℃ (or room temperature) pH value: 4.0-9.0

[0022] Action time: 1-6h Addition: 500g-5000g tons of materials

[0023] 5. Application effect:

[0024] The application of the present invention and the use of biological methods essentially solve the fatal problems of low ex...

Example Embodiment

[0030] Example 1: The specific formula of the present invention is as follows (the content is the weight percentage of the ingredients): 50% protease, 30% cellulase, 10% pectinase, and 10% xylanase.

[0031] The preparation method is as follows: the raw enzymes are respectively subjected to pretreatment, microfiltration membrane filtration, and then proportionally fed into the automatic mixer for compounding, and then filtered through the fine filtration membrane and quantitatively filled to become products.

Example Embodiment

[0032] Example 2: The specific formula of the present invention is as follows (the content is a weight percentage component): 30% protease, 30% cellulase, 30% pectinase, and 10% xylanase. The preparation method is the same as in Example 1.

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Abstract

The invention relates to a complex enzyme formula for improving the extraction rate of sodium alginate and application, and belongs to the technical field of enzymology. The complex enzyme consists of a plurality of biological enzymes, is suitable for the industrial production of extracting and separating the sodium alginate from kelp and sargasso, can improve the extraction rate and the viscosity of the sodium alginate in the kelp and the sargasso, and simultaneously reduces the dosage of the chemical reagents.

Description

technical field [0001] The invention relates to a compound enzyme formula capable of improving the extraction rate of sodium alginate in kelp and sargassum and its application. The formula is as follows: protease 30-50%, cellulase 10-30%, pectinase 10-30%, Xylanase 5-15%. technical background [0002] Sodium alginate is a polysaccharide carbohydrate extracted from brown algae kelp or sargassum, which is composed of 1,4-poly-β-D-mannuronic acid and α-L-zanuronic acid. A linear polymer, one of alginic acid derivatives. Its molecular formula is (C 6 h 7 o 6 Na) n , and the relative molecular weight is around 32,000 to 200,000. [0003] Sodium alginate is widely used in chemistry, biology, medicine, food and other fields due to its good biodegradability and biocompatibility. At present, the methods of extracting sodium alginate in the world can be divided into four kinds of processes, namely: acid coagulation acidification method, calcium coagulation acidification method,...

Claims

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Application Information

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IPC IPC(8): C12N9/00C12N9/50C12N9/42C12N9/26C12N9/88C08B37/04
Inventor 赵岩张连学吴鹏蔡恩博郜玉钢
Owner SHANDONG GANGYUAN MARINE BIOENG CO LTD
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