Method for extracting L-threonine

A technology for threonine and threonine fermentation broth, applied in the field of biochemical industry, can solve the problems of inconvenient operation, cumbersome steps, influence on the extraction purity of L-threonine, etc., and achieve the effects of simple steps and improved purity

Active Publication Date: 2011-04-06
梅花生物科技集团股份有限公司
View PDF3 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in this method, the fermentation broth needs to be decolorized, the steps are cumbersome, and the operation is inconvenient, and the mother liq

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting L-threonine
  • Method for extracting L-threonine

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0032] Example 1: The method for extracting L-threonine according to the present invention

[0033] 1. Extraction method

[0034] The L-threonine fermentation broth is sterilized and filtered through a ceramic membrane. The ceramic membrane has a pore size of 50nm, a molecular weight of 30KD, an operating pressure of 1.5bar, a transmembrane pressure difference of 1bar, and a temperature of 25℃. The filtrate and L-threonine The volume ratio of the fermentation broth is 1:4.

[0035] The obtained L-threonine filtrate enters the four-effect evaporation for preliminary concentration, the vacuum degree is controlled at -0.092Mpa, and the temperature is controlled to 50℃, 60℃, 70℃, and 80℃ respectively, and finally the Baume concentration of the filtrate is controlled to reach 7. Then enter the single-effect evaporator for further concentration, control the temperature at 55°C, control the vacuum degree at -0.092Mpa, the stirring speed 80rpm, concentrate until the filtrate solid-liquid ra...

Example Embodiment

[0039] Example 2: The method for extracting L-threonine according to the present invention

[0040] 1. Extraction method

[0041] The L-threonine fermentation broth is sterilized and filtered through a ceramic membrane. The ceramic membrane has a pore size of 50nm, a molecular weight of 30KD, an operating pressure of 1.5bar, a transmembrane pressure difference of 1bar, and a temperature of 25℃. The filtrate and L-threonine The volume ratio of the fermentation broth is 1:6.

[0042] The obtained L-threonine filtrate enters the four-effect evaporation for preliminary concentration, the vacuum degree is controlled at -0.092Mpa, the temperature is controlled to 50℃, 60℃, 70℃, and 80℃ respectively, and the baume concentration of the filtrate is finally controlled to reach 8. Then enter the single-effect evaporator for further concentration, control the temperature at 58°C, control the vacuum at -0.092Mpa, stir at 80rpm, concentrate until the filtrate solid-liquid ratio reaches 0.75:1, pu...

Example Embodiment

[0046] Example 3: The method for extracting L-threonine according to the present invention

[0047] 1. Extraction method

[0048] The L-threonine fermentation broth is sterilized and filtered through a ceramic membrane. The ceramic membrane has a pore size of 50nm, a molecular weight of 30KD, an operating pressure of 1.5bar, a transmembrane pressure difference of 1bar, and a temperature of 25℃. The filtrate and L-threonine The volume ratio of the fermentation broth is 1:5.

[0049] The obtained L-threonine filtrate enters the four-effect evaporation for preliminary concentration, the vacuum degree is controlled at -0.092Mpa, the temperature is controlled to 50℃, 60℃, 70℃, and 80℃ respectively, and the baume concentration of the filtrate is finally controlled to 9. Then enter the single-effect evaporator for further concentration, control the temperature at 57℃, control the vacuum degree at -0.092Mpa, the stirring speed 80rpm, concentrate until the filtrate solid-liquid ratio reaches...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Apertureaaaaaaaaaa
Molecular weightaaaaaaaaaa
Login to view more

Abstract

The invention relates to the field of biochemical engineering, in particular to a method for extracting L-threonine. The method comprises the following steps of: (1) micro-filtrating L-threonine fermentation liquor, concentrating a filtrate to a solid-to-liquid ratio of 0.45-0.75 to 1 and centrifugating to obtain L-threonine crystals and mother liquor; (2) carrying out cross-adsorption on the mother liquor, then, eluting with ammonia with a pH value of 10-14 and collecting eluant from the start of elution to the Baume degree of 1; and (3) adding the L-threonine fermentation liquor to the eluant in the step (2), carrying out micro-filtration, concentrating the filtrate to the solid-to-liquid ratio of 0.45-0.75 to 1, centrifugating to obtain L-threonine crystals and mother liquor and repeating the step (2) to the mother liquor. The method of the invention is simple and convenient for operation without bleaching and can obtain high-quality L-threonine through separation by controlling the concentration times, and the crystallized mother liquor after the cross-adsorption is mixed and cycled with the L-threonine fermentation liquor to improve the purity of the subsequently extracted L-threonine.

Description

technical field [0001] The invention relates to the field of biochemical industry, and specifically provides a method for extracting L-threonine. Background technique [0002] Threonine, the scientific name is 2-amino-3-hydroxybutyric acid, the English name is Threonine Itaconic acid, and the molecular formula is C 4 h 9 o 3 N, its properties are white orthorhombic crystal or crystalline powder, odorless, slightly sweet taste, soluble in water, insoluble in ethanol, ether and chloroform. Threonine is one of the 20 kinds of amino acids that make up proteins. It has two asymmetric carbon atoms and can have 4 isomers. However, L-threonine exists naturally and has physiological effects on organisms. Since humans and animals cannot synthesize L-threonine themselves, they need to be ingested from food, so L-threonine is an essential amino acid, which is mainly used in food fortifiers, medicines, feed additives, etc. [0003] As an important food fortifier, L-threonine can stre...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07C229/22C07C227/40
Inventor 钟秀茹王海雷龚华常利斌
Owner 梅花生物科技集团股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products