Method for extracting L-threonine
A technology for threonine and threonine fermentation broth, applied in the field of biochemical industry, can solve the problems of inconvenient operation, cumbersome steps, influence on the extraction purity of L-threonine, etc., and achieve the effects of simple steps and improved purity
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[0032] Example 1: The method for extracting L-threonine according to the present invention
[0033] 1. Extraction method
[0034] The L-threonine fermentation broth is sterilized and filtered through a ceramic membrane. The ceramic membrane has a pore size of 50nm, a molecular weight of 30KD, an operating pressure of 1.5bar, a transmembrane pressure difference of 1bar, and a temperature of 25℃. The filtrate and L-threonine The volume ratio of the fermentation broth is 1:4.
[0035] The obtained L-threonine filtrate enters the four-effect evaporation for preliminary concentration, the vacuum degree is controlled at -0.092Mpa, and the temperature is controlled to 50℃, 60℃, 70℃, and 80℃ respectively, and finally the Baume concentration of the filtrate is controlled to reach 7. Then enter the single-effect evaporator for further concentration, control the temperature at 55°C, control the vacuum degree at -0.092Mpa, the stirring speed 80rpm, concentrate until the filtrate solid-liquid ra...
Example Embodiment
[0039] Example 2: The method for extracting L-threonine according to the present invention
[0040] 1. Extraction method
[0041] The L-threonine fermentation broth is sterilized and filtered through a ceramic membrane. The ceramic membrane has a pore size of 50nm, a molecular weight of 30KD, an operating pressure of 1.5bar, a transmembrane pressure difference of 1bar, and a temperature of 25℃. The filtrate and L-threonine The volume ratio of the fermentation broth is 1:6.
[0042] The obtained L-threonine filtrate enters the four-effect evaporation for preliminary concentration, the vacuum degree is controlled at -0.092Mpa, the temperature is controlled to 50℃, 60℃, 70℃, and 80℃ respectively, and the baume concentration of the filtrate is finally controlled to reach 8. Then enter the single-effect evaporator for further concentration, control the temperature at 58°C, control the vacuum at -0.092Mpa, stir at 80rpm, concentrate until the filtrate solid-liquid ratio reaches 0.75:1, pu...
Example Embodiment
[0046] Example 3: The method for extracting L-threonine according to the present invention
[0047] 1. Extraction method
[0048] The L-threonine fermentation broth is sterilized and filtered through a ceramic membrane. The ceramic membrane has a pore size of 50nm, a molecular weight of 30KD, an operating pressure of 1.5bar, a transmembrane pressure difference of 1bar, and a temperature of 25℃. The filtrate and L-threonine The volume ratio of the fermentation broth is 1:5.
[0049] The obtained L-threonine filtrate enters the four-effect evaporation for preliminary concentration, the vacuum degree is controlled at -0.092Mpa, the temperature is controlled to 50℃, 60℃, 70℃, and 80℃ respectively, and the baume concentration of the filtrate is finally controlled to 9. Then enter the single-effect evaporator for further concentration, control the temperature at 57℃, control the vacuum degree at -0.092Mpa, the stirring speed 80rpm, concentrate until the filtrate solid-liquid ratio reaches...
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