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Highly metastatic model of human melanoma, cell subline, creation methods, and dynamic detection of metastasis

A technology for melanoma and malignant melanoma, which is applied in the field of high-metastatic human melanoma models, cell sub-lines, and dynamic detection of establishment and metastasis, and can solve the problems that restrict the progress of human melanoma metastasis mechanism and the exact mechanism is not very clear.

Inactive Publication Date: 2013-07-10
SHANGHAI INST OF ONCOLOGY
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  • Abstract
  • Description
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Problems solved by technology

However, the exact mechanism of tumor metastasis is still not very clear, and tumor metastasis has always been a hot spot in oncology research.
Therefore, the relatively large limitations in the research in this field have restricted the progress of research on the mechanism of human melanoma metastasis.

Method used

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  • Highly metastatic model of human melanoma, cell subline, creation methods, and dynamic detection of metastasis
  • Highly metastatic model of human melanoma, cell subline, creation methods, and dynamic detection of metastasis
  • Highly metastatic model of human melanoma, cell subline, creation methods, and dynamic detection of metastasis

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Embodiment Construction

[0097] In order to make the technical means, creative features, goals and effects of the present invention easy to understand, the present invention will be further described below in conjunction with specific illustrations.

[0098] 1. In vivo screening of high metastasis model of human melanoma SCID mice transplanted subcutaneously

[0099] 1.1 Materials

[0100] 1.1.1 Animals SCID mice (T and B cell functional defects) were provided by Shanghai Cancer Institute [production license number is SCXK (Shanghai) 2007-0001]. Rats aged 6-8 weeks, weighing 18-22g, male and female, the test and breeding strictly complied with the requirements of the SPF standard [use license number is SYXK (Shanghai) 2007-0001].

[0101] 1.1.2 Cells A375 melanoma cells were long-term preserved and passaged by the Shanghai Cancer Institute according to conventional methods (obtained from the cell bank of Shanghai Institute of Biological Sciences, Chinese Academy of Sciences in September 2000, cell nu...

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Abstract

The invention discloses a highly metastatic model of human melanoma, a highly metastatic cell subline of the human melanoma, creation methods for the highly metastatic model and the highly metastatic cell subline, and the dynamic detection of metastasis. The subcutaneously-transplanted mouse highly metastatic model and the corresponding cell subline are established in an in-vivo screening way in a mouse with severe combined immune deficiency (SCID) by using mouse lung metastasis, namely human malignant melanoma cell strain A375 pulmonary metastasis, wherein the highly metastatic cell subline of the human melanoma is A375sci, and has a human tumor cell karyotype; and 60 to 75 hypo-triploid-dominated chromosomes are acrocentric and have a heteroploid karyotype. The cell subline has the two routes of metastasis of blood trails and lymph. The in-vivo screening of the highly-metastatic model is performed by using animals with severe immune deficiency, and is expressed and applied in nude mice. A method for detecting Alu genes by using a polymerase chain reaction (PCR) method is simple, highly sensitive and highly specific, and can be used for detecting organ metastasis, particularly micrometastasis, in a human tumor animal-xenotransplantation model.

Description

technical field [0001] The invention relates to a high-metastasis model of human melanoma, a cell subline, a method for establishing it, and dynamic detection of metastasis, and belongs to the technical field of animal models and animal cell lines. Background technique [0002] Tumor invasion and metastasis are one of the most basic biological characteristics of malignant tumors. Death from primary tumors is relatively rare, and more than 90% of tumor patients die from the metastasis of tumor cells in the human body. [0003] Human melanoma is one of the main diseases that harm the head and neck, becoming the most common malignant tumor. However, the exact mechanism of tumor metastasis is not very clear at present, and tumor metastasis has always been a hot spot in oncology research. Therefore, the relatively large limitations in the research in this field have restricted the progress of the research on the mechanism of human melanoma metastasis. Contents of the invention...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/09
Inventor 姚明王晓敏闫明霞孔韩卫刘蕾萨冰清李静
Owner SHANGHAI INST OF ONCOLOGY
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