Array micro polymerase chain reaction chip

A technology of chain reaction and polymerase, applied in the field of deoxyribonucleic acid amplification, to achieve the effect of flexible and convenient use, easy to learn, and avoid pollution

Inactive Publication Date: 2011-05-25
INST OF ELECTRONICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
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Problems solved by technology

[0006] The purpose of the present invention is to disclose a kind of array type micro-polymerase chain reaction (PCR) chip, to overcome the shortcoming of traditional desktop amplification instrument and solve the problem that miniatur

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  • Array micro polymerase chain reaction chip
  • Array micro polymerase chain reaction chip

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Embodiment Construction

[0029] An array micro-polymerase chain reaction (PCR) chip, using micro-electro-mechanical systems (MEMS) technology and materials (such as silicon, platinum, etc.) The micro-reaction chamber array is made of methane (PDMS) material, and the micro-heating chip is separated from the micro-reaction chamber array. Every time an amplification reaction is performed, take a clean micro-reaction chamber array and stick it on the micro-heating chip, and control the temperature cycle of the micro-heating chip through the temperature control system to realize the amplification reaction.

[0030] A kind of array type micro-polymerase chain reaction (PCR) chip of the present invention mainly comprises following three parts, and the structure and function of every part are as follows:

[0031] (1) Micro heating chip. The micro-heating chip is made using micro-electro-mechanical system (MEMS) technology and materials. The main process includes the steps of growing silicon dioxide and silic...

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Abstract

The invention discloses an array micro polymerase chain reaction chip, and relates to desoxyribonucleic acid (DNA) amplification technology. The chip comprises a temperature control system, a heating chip and a reaction cavity; the heating chip and the reaction cavity are arranged separately; and during use, after a reaction cavity framework is sleeved on the external circle of an insulating substrate, the reaction cavity framework is pressed down so that the lower surface of the bottom of a reaction tank is tightly jointed with the upper surface of a heating electrode, sample solution is injected into the reaction tank, and then the chip is started. The amplification reaction can be performed by sticking a clean disposable polydimethylsiloxane (PDMS) micro array to the heating chip every time. The reaction chip can microminiaturize polymerase chain reaction (PCR), fulfills the purposes of microminiaturization, lightness, quickness and saving, and can also solve the problems of biocompatibility difficult to be overcome by microminiaturization and pollution of the PCR.

Description

technical field [0001] The invention relates to the technical field of deoxyribonucleic acid (DNA) amplification, and the main content is to realize rapid amplification of specific deoxyribonucleic acid (DNA) fragments in vitro by using polymerase chain reaction technology. Background technique [0002] Polymerase chain reaction technology can realize rapid amplification of special deoxyribonucleic acid (DNA) fragments in vitro, and is widely used in many fields such as life sciences, biomedicine, and forensic identification. [0003] After decades of development, polymerase chain reaction (PCR) technology has become increasingly mature. The bench-top polymerase chain reaction (PCR) amplification instrument sold on the market is also of a great variety with different functions. However, they generally have the disadvantages of large volume, heavy mass, slow heating and cooling rate, and large amount of reagents required. Using micro-electro-mechanical system (MEMS) technol...

Claims

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Application Information

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IPC IPC(8): C12M1/38C12M1/34C12M1/00
Inventor 赵湛丁国杰于留波方震
Owner INST OF ELECTRONICS CHINESE ACAD OF SCI
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