Array micro polymerase chain reaction chip

Abstract

The invention discloses an array micro polymerase chain reaction chip, and relates to desoxyribonucleic acid (DNA) amplification technology. The chip comprises a temperature control system, a heating chip and a reaction cavity; the heating chip and the reaction cavity are arranged separately; and during use, after a reaction cavity framework is sleeved on the external circle of an insulating substrate, the reaction cavity framework is pressed down so that the lower surface of the bottom of a reaction tank is tightly jointed with the upper surface of a heating electrode, sample solution is injected into the reaction tank, and then the chip is started. The amplification reaction can be performed by sticking a clean disposable polydimethylsiloxane (PDMS) micro array to the heating chip every time. The reaction chip can microminiaturize polymerase chain reaction (PCR), fulfills the purposes of microminiaturization, lightness, quickness and saving, and can also solve the problems of biocompatibility difficult to be overcome by microminiaturization and pollution of the PCR.

Description

technical field [0001] The invention relates to the technical field of deoxyribonucleic acid (DNA) amplification, and the main content is to realize rapid amplification of specific deoxyribonucleic acid (DNA) fragments in vitro by using polymerase chain reaction technology. Background technique [0002] Polymerase chain reaction technology can realize rapid amplification of special deoxyribonucleic acid (DNA) fragments in vitro, and is widely used in many fields such as life sciences, biomedicine, and forensic identification. [0003] After decades of development, polymerase chain reaction (PCR) technology has become increasingly mature. The bench-top polymerase chain reaction (PCR) amplification instrument sold on the market is also of a great variety with different functions. However, they generally have the disadvantages of large volume, heavy mass, slow heating and cooling rate, and large amount of reagents required. Using micro-electro-mechanical system (MEMS) technol...

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to disclose a kind of array type micro-polymerase chain reaction (PCR) chip, to overcome the shortcoming of traditional desktop amplification instrument and solve the problem that miniaturization polymerase chain reaction (PCR) faces, this chip performance is stable, Inexpensive and small in size, the micro-heating chip and the micro-reaction chamber array are separated, and the amplification reaction is simple and fast

Images