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Method for screening carambola bacterial spot germ antagonistic bacteria by using chloroform

A spot germ and chloroform technology, which is applied in the preservation of microorganisms, etc., can solve the problems of long screening time and unstable screening effect, and achieve the effect of simple and fast operation, stable effect and simple operation

Inactive Publication Date: 2013-03-13
INST OF PLANT PROTECTION FAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Aiming at the problems in the prior art that the screening of carambola bacterial spot antagonistic bacteria takes a long time and the screening effect is unstable, the present invention provides a method for screening carambola bacterial spot antagonistic bacteria using chloroform, which is easy to operate , less time-consuming and more stable results

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  • Method for screening carambola bacterial spot germ antagonistic bacteria by using chloroform

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Experimental program
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Embodiment 1

[0024] (1) Culture of possible antagonistic bacteria

[0025] Use an inoculation loop to inoculate the sample strains ZB-77, ZB-80, ZB-82, ZB-88, ZB-95, ZB-142, ZB-178 in the center of the NA medium plate, and culture them upside down at 28°C 72h; the strains were respectively connected to 3 dishes for parallel screening;

[0026] (2) Cultivation of Carambola Bacterial Spot

[0027] Inoculate carambola bacterial spot bacteria in 200mL LB liquid medium, culture at 28°C, shake at 160r / min, and adjust the concentration to 10 with sterile water after culturing for 24 hours. 8 cfu / mL bacterial solution;

[0028] (3) Use chloroform to fumigate the cultured antagonistic bacteria

[0029] Pour 3mL of chloroform into a new dish cover, place the cultured sample strain plate upside down on the dish cover, fumigate for 8-10min, open the dish cover, and discard the remaining liquid;

[0030] (4) Inoculation of pathogenic bacteria liquid

[0031] Spread the diluted carambola bacterial ...

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Abstract

The invention provides a method for screening carambola bacterial spot germ antagonistic bacteria by using chloroform. The method comprises the following steps: firstly, culturing strains for a period of time, so that the components of the strains release metabolic products in a culture medium; poisoning the metabolic products by using the chloroform; and then, inoculating pathogenic bacteria for culturing so as to observe the condition of growth. The method can be used for effectively and stably screening the pathogenic bacteria, lays a foundation for biological control of carambola bacterial spot diseases, and provides reliable technical and theoretical bases.

Description

technical field [0001] The invention relates to a method for screening carambola bacterial spot pathogen antagonistic bacteria by using chloroform, and belongs to the field of biological control technology of crop diseases. Background technique [0002] Carambola bacterial spot is caused by carambola bacterial spot pathogenic bacteria ( Pseudomonas syringae ) is a disease caused by infection. Carambola (Averrhoa carambola L.) belongs to the Oxalis family and belongs to tropical and subtropical evergreen tree fruit trees. It is widely planted in Guangdong, Hainan and other provinces of my country. In recent years, some carambola plantations or nurseries in Guangdong and Hainan provinces have discovered a leaf spot disease that causes severe leaf defoliation. Leaf spot tissue was cut out for microscopic examination, and bacterial overflow was found, which was considered to be a bacterial disease. The disease has not been reported at home and abroad, so it is necessary to st...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/04
Inventor 林静兰成忠陈庆河李本金李炜翁启勇
Owner INST OF PLANT PROTECTION FAAS