Supercharge Your Innovation With Domain-Expert AI Agents!

Enzymatic analytical membrane, test device and method

A technology for enzyme analysis, analyte, applied in measurement devices, biochemical equipment and methods, enzymology/microbiology devices, etc., which can solve problems such as confusion

Active Publication Date: 2011-06-08
ZBX CORP
View PDF45 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Presence of other colored bodies such as red blood cells can confound results

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Enzymatic analytical membrane, test device and method
  • Enzymatic analytical membrane, test device and method
  • Enzymatic analytical membrane, test device and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] Example 1: Evaluation of Enzyme Assays for the Detection of Acetaminophen (APAP) and Methanol (MeOH) membrane

[0082] Twelve sera from two different MeOH overdoses and 17 sera positive for APAP by immunoassay were analyzed by the enzymatic assay membrane described herein. In addition to commercially available quality control samples, 6 and 10 sera that were negative for MeOH and APAP, respectively, were also analyzed. Cross-reactivity to ethanol was tested with 40 mmol, 80 mmol and 120 mmol of ethanol. Inspection technicians ignore initial results.

[0083] MeOH: Nine out of eleven POCT positive samples were positive according to the results of gas chromatography (GC). The results are read at two minutes. The two samples that were positive by the enzyme assay membrane and negative by GC were only below the detection limit of the GC, suggesting that the enzyme assay membrane may be more sensitive than the GC. The detection limit of GC is about 2 mM. Consistent ...

Embodiment 2

[0086] Example 2: Membrane device for acetaminophenase analysis

[0087] An acetaminophen detection device using a drop of whole blood sample was prepared according to the present invention. For the signal area, nitrocellulose (Millipore) was infiltrated with the chromogenic reagent at a specified flow rate of 180 sec / 4 cm using a conventional liquid sprayer. Chromogenic reagents included 3% o-cresol, 6.4 mM copper sulfate and 1M sodium hydroxide. The infiltrated nitrocellulose was left for 30 minutes at room temperature at less than 20% relative humidity. The separation zone (Whatman) was sprayed with enzyme solution and then lyophilized to remove water. The enzyme solution included 250 U / mL aryl acyl amidase (GDS Technology). Enzyme assay membranes were supported on polystyrene liners (G & L Precision Die Cutting, Inc.). Use a die-cut tool to obtain the shape of the enzymatic assay membrane. Such as image 3 As shown, place the enzyme analysis membrane in the analysis ...

Embodiment 3

[0088] Example 3: Membrane device for methanolase analysis

[0089] A methanol detection device using a drop of whole blood sample was prepared according to the present invention. For the signal area, infiltrate nitrocellulose at a prescribed flow rate of 180 sec / 4 cm with chromogenic reagent, 18 U / mL formaldehyde dehydrogenase (Sigma-Aldrich) and 6 mM β-nicotinamide adenine dinucleotide hydrate (β-NAD) ( Millipore). Chromogenic reagents included 0.3 mM phenazine methyl sulfate, 1.2 mM nitro blue tetrazolium chloride and 0.5% Triton X-100. The separation zone (Whatman) was sprayed with 315 U / mL alcohol oxidase solution (Sigma-Aldrich). The soaked membranes were left for 30 minutes at room temperature at a relative humidity below 20%. Enzyme assay membranes were supported on polystyrene liners (G & L Precision Die Cutting, Inc.). Use a die-cut tool to obtain the shape of the enzymatic assay membrane. Such as image 3 As shown, place the enzyme analysis membrane in the a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention is directed to a novel enzymatic analytical membrane, a lateral flow enzymatic detection method, and an analytical device incorporating same. The invention is useful for rapidly enzymatically determining the presence of one or more analytes in small volumes of sample. The invention provides an enzymatic analytical membrane for detecting the presence of one or more small-molecule analytes in a biological sample where the membrane comprises a receiving zone; a separation zone and a signal zone, at least one of the zones comprising one or more enzymes for converting the analytes into a form detectable by reaction with a chromogenic agent present in the signal zone and wherein the membrane horizontally receives sample at the receiving zone, and the sample continues via lateral flow through the receiving zone, separation zone and signal zone where a visible color change is formed indicating the presence of the analyte.

Description

[0001] This application claims priority to US Provisional Patent Application No. 61 / 071921, filed May 27, 2008, which is hereby incorporated by reference in its entirety. field of invention [0002] The present invention relates to analytical membranes, methods and devices for the detection of analytes in fluid samples. More specifically, the present invention relates to a novel enzymatic analytical membrane, a lateral flow enzyme detection method, and an analytical device including the enzymatic analytical membrane. The present invention is useful for rapid enzymatic detection of the presence of one or more analytes in small volume samples. Background of the invention [0003] Throughout this application, various references are cited in parentheses to more fully describe the state of the art to which this invention pertains. The disclosures of these references are hereby incorporated by reference in their entirety. [0004] If left untreated, acetaminophen (APAP) and meth...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/25C12M1/34C12M1/40C12Q1/26C12Q1/34G01N21/78C12N15/11
CPCC12Q1/26C12Q1/34G01N21/78G01N33/558G01N33/523G01N33/521
Inventor 史沁卫
Owner ZBX CORP
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com