Kit for detecting susceptibility to leukemia

A kit and susceptibility technology, applied in the field of kits for detecting susceptibility to leukemia, can solve problems such as bad living habits, and achieve the effects of high sensitivity, high throughput and easy operation

Active Publication Date: 2011-06-29
中源协和基因科技有限公司
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Problems solved by technology

[0009] The technical problem to be solved by the present invention is to comprehensively detect and analyze a group of genes and loci related to leukemia susceptibility, using specific primers and probes, and combining single nucleotide extension t

Method used

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  • Kit for detecting susceptibility to leukemia
  • Kit for detecting susceptibility to leukemia
  • Kit for detecting susceptibility to leukemia

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Embodiment Construction

[0057] The present invention will be described in further detail below in conjunction with specific embodiments. For the scale-up of experiments without specific conditions indicated in the following examples, conventional conditions or the conditions suggested by the manufacturer are generally followed.

[0058] 1 DNA extraction:

[0059] Take the peripheral venous blood of the subject, add the same volume of cell lysate, and lyse twice to fully lyse the white blood cells. After centrifuging and discarding the supernatant, add proteinase K buffer and proteinase K (50ug / ml), incubate at 65°C for 10 minutes, iso Precipitate with propanol, wash twice with 75% ethanol, dry and dissolve in appropriate amount of TB solution.

[0060] 2PCR amplification

[0061] Take the subject's genomic DNA extract and add it to a 96-well plate for multiplex PCR amplification:

[0062] The total reaction volume is 5ul, of which 10M mol / L dNTPs 0.0375ul, 10×PCR Buffer0.5ul, 25mmol / L MgCl 2 1ul,...

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Abstract

The invention discloses a kit for detecting susceptibility to leukemia. The kit is used for detecting three types of genes closely related to leukemia, namely CYP1A1 genes and NQO1 genes which are related to toxicant metabolization, and XRCC1 genes related to DNA restoration. SNP loci which can be detected simultaneously include rs4646903 loci of CYP1A1 genes, rs1800566 loci of NQO1, and rs1799782 and rs25487 loci of XRCC1. The kit is used, particularly a group of genes and loci which are related to the susceptibility to leukemia are detected, a primer with specificity and a probe are utilized and mononucleotide extension technology is adopted in combination with micro-array chip technology, so that whether persons to be detected carry 'leukemia susceptibility gene' or not can be judged, persons who are susceptible to leukemia are screened, bad living habits are changed and the prevention purpose is achieved.

Description

technical field [0001] The present invention relates to a kit for detecting disease susceptibility, especially a kit for detecting susceptibility to leukemia. By detecting single nucleotide polymorphism (SNP) of CYP1A1, NQO1 and XRCC1, individual susceptibility to leukemia. Background technique [0002] Leukemia has a high incidence rate in the Chinese population and is the most common malignant tumor in children and young adults. In recent years, its incidence rate is on the rise, and the etiology has not yet been elucidated. The current accepted view is that leukemia is caused by environment-gene interaction. Genetic aspects involve mutations in metabolism, DNA damage repair genes, etc. Studies have found that the metabolic enzyme family in the carcinogen metabolic pathway is polymorphic in the population, and different individuals have huge differences in their ability to metabolize specific carcinogens. Likewise, there are significant individual differences in DNA re...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 韩俊领刘志霈周毓玲杜宏伟李楠靳霞刘蓉华
Owner 中源协和基因科技有限公司
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