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Method for extracting lycopene continuously
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A continuous technology of lycopene, applied in the field of bio-separation, can solve the problems of inability to realize industrialized production, large loss of lycopene, easy oxidation of lycopene, etc., achieve low cost, short extraction time, and reduce the impact of instability Effect
Active Publication Date: 2013-12-18
NANJING TECH UNIV
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Problems solved by technology
Most of the extraction methods currently reported are organic solvent extraction, which consumes a lot of solvents. During the extraction process, lycopene is easily oxidized under the influence of light and air, and the loss of lycopene is relatively large.
Patent CN1760282A reports supercritical CO 2 Extraction of lycopene has high extraction rate, no solvent residue, and short extraction time, but industrial production cannot be realized due to expensive equipment and high cost
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Embodiment 1
[0057] A single colony of the original strain of Blaleslea trispora was inoculated from a slant into a 500mL shake flask for seed culture, with a liquid volume of 10%, culture temperature 30°C, rotation speed 200r / min, and culture for 30h. Inoculate the primary shake flask seed solution into a 2.5L shake flask for secondary seed cultivation. The cultivation temperature is maintained at 30°C, the rotation speed is 200r / min, and the cultivation is carried out for 30h. After the seeds are cultivated, they are inoculated into a 50L fermenter for fermentation and cultivation. The filling coefficient is 60%, the filling volume is 30L, the cultivation temperature is maintained at 30°C, the rotation speed is 200r / min, the ventilation rate is 0.2-0.5L / min, and the cultivation is carried out for 120h.
[0058] After the fermentation was completed, 28L of fermented liquid was obtained, and excessive moisture in the fermented liquid was removed by centrifugation to obtain 3kg of wet myceli...
Embodiment 2
[0060] Take 10 g of the pretreated mycelium described in Example 1, put it in a 500 mL round bottom flask, add 400 mL of n-hexane / acetone mixed extraction solvent, stir and extract at 40 ° C for 1 hour, and extract three times. The extraction of lycopene under these operating conditions The rate reached 81.42%.
[0061] Take 50 g of the pretreated mycelium described in Example 1 and put it into a chromatographic column, add 500 mL of n-hexane / acetone mixed solvent, extract in a countercurrent circulation at a flow rate of 1.2 BV / h at 30 ° C for 1.5 h, and reflux three times. The extraction rate of lycopene reached 88% under the operating conditions.
Embodiment 3
[0063] Get the mycelium 250g of pretreatment described in embodiment 1, add such as figure 1 In the chromatographic columns of the continuous extraction system with 5 extraction units shown, unit 5 adds fresh solvent for a stage of extraction for 1.5 hours and then discharges the extract solution, and units 2-4 perform a stage of 22.5min, 45min, and 67.5min respectively Extraction, exchange the solvents of units 2 and 4, and finally add fresh solvent in unit 5, so that each unit according to figure 2 The concentration gradient arrangement shown; continue to extract for 22.5 minutes and carry out slagging charging to the unit 5 that has been extracted, and transfer the saturated solvent of unit 1 to the liquid storage tank; Migrate to the next unit in the opposite direction; fill unit 4 with fresh solvent after migration. The extraction conditions of each unit were as follows: temperature 30°C, 5 extraction unit groups, 10mL / g mycelia solvent for each group, countercurrent ci...
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Abstract
The invention belongs to the field of biological separation, and discloses a method for extracting lycopene continuously, which comprises the following steps of: adding blakeslea trispora fermented mycelia into ethanol for dehydrating, filtering and separating the mycelia from ethanol treating fluid; drying the wet mycelia under vacuum and crushing to obtain dried mycelia; adding the dried mycelia into mixed solvent to perform solid-liquid phase extraction; adding the mycelia into each extracting unit of a continuous extracting system, circulating continuously by mixed extraction solvent, and extracting for a certain time period, arranging all units according to the gradient of concentration, performing multi-stage countercurrent extraction until the lycopene is extracted completely, and collecting extract liquor; and concentrating the extract liquor under vacuum to obtain lycopene concentrated liquor. In the method, the lycopene is extracted by the mixed solvent, so that the dissolution effect of the solvent on the lycopene is played fully; the operation is continuous and can be performed in dark places; and the method consumes less solvent consumption, has large treatment capacity, short extraction time and low energy consumption, is low in cost and facilities industrial production.
Description
technical field [0001] The invention belongs to the field of biological separation and relates to a method for continuously extracting lycopene. The method can be used to effectively extract lycopene from the fermented mycelium of the mucormycete fungus Blaleslea trispora. Background technique [0002] Lycopene is a fat-soluble natural pigment belonging to carotenoids. Lycopene has more superior properties than other carotenoids, such as its strongest antioxidant activity among carotenoids, and its ability to quench singlet oxygen is V E 100 times that of β-carotene, more than 2 times that of β-carotene, and the ability to scavenge free radicals is also better than other carotenoids. It has a strong antioxidant effect, can inhibit the growth of cancer cells, slow down the oxidation of low-density lipoprotein (LDL), prevent arteriosclerosis, protect the cardiovascular system, and remove toxic substances in cigarettes and car exhaust. [0003] The production methods of lyco...
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