Method for screening and testing activity of lysine propionylation removal enzyme and butyrylation removal enzyme

A technology for removing butyrylase and propionylase, which can be used in the determination/inspection of microorganisms, biochemical equipment and methods, measuring devices, etc., and can solve problems such as research and affecting biological functions.
CN102140494BActive Publication Date: 2015-07-08JINGJIE PTM BIOLAB HANGZHOU CO LTD
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Patents(China)
Current Assignee / Owner
JINGJIE PTM BIOLAB HANGZHOU CO LTD
Publication Date
2015-07-08

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Abstract

The invention relates to a method for screening and testing activity of a lysine propionylation removal enzyme and a butyrylation removal enzyme. The basis thought of the method is that: lysine propionylation or butyrylation modification degree of a substrate can be reflected by the sensitivity of the substrate to the catalysis removal enzyme. The method comprises the following steps of: a, lysine propionylation substrate polypeptide marked with releasable signal substances; b, the lysine propionylation removal enzyme; and c, a detection method for distinguishing the modification lysine propionylation polypeptide from corresponding non-modification polypeptide. The method can be used for screening modification removal enzymes and testing the activity of the enzymes, and also can screen substrates influencing the activity of the enzymes to be further used in quick diagnosis of several diseases.
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Description

technical field

[0001] The invention relates to a method for screening lysine depropionylase and debutyrylase and measuring the activities of lysine depropionylase and debutyrylase. Especially by measuring Boc-Lys(Prop)-AMC (tert-butyroxybutylated lysine-4-amino-7-methylcoumarin) and Boc-Lys(Buty)-AMC (tert-butyroxy Fluorescence intensity of propionylated lysine-4-amino-7-methylcoumarin) catalyzed by lysine depropionylase and debutyrylase, combined with mass spectrometry identification, biochemical analysis and Cytological assays confirmed specific lysine depropionylase and debutyrylase and assayed for enzyme activity. Background technique

[0002] The nucleosome is the basic repeating unit of chromatin, consisting of an octameric core histone surrounded by approximately 147 base pairs of DNA. Nucleosomes are capable of further folding into higher order structures (1) . Dynamic changes in chromatin structure regulate DNA plasticity, which in turn affects DNA template-bas...

Claims

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