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Bacillus amyloliquefaciens preparation for controlling viral diseases of plants and application thereof

An anti-plant virus and virus disease technology, applied in plant growth regulators, plant growth regulators, applications, etc., can solve problems such as problems in practical applications, and achieve the goal of inhibiting virus replication and proliferation, improving adsorption, and reducing disease transmission. Effect

Inactive Publication Date: 2011-08-24
TOBACCO RES INST CHIN AGRI SCI ACAD +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Chinese literature "Research on the inhibition of tobacco mosaic virus RNA accumulation in leaves by biocontrol bacteria Ba33," Plant Protection, 2010, 36(3): 82-84." Action, Journal of Jilin Agricultural University, 2010, 32(4): 383-386." It is recorded that Bacillus amylobacter Ba33 has an inhibitory effect on tobacco mosaic virus (TMV), but it is only a mechanism study and has not been made Preparations suitable for industrial production and application, but there are difficulties in their practical application

Method used

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  • Bacillus amyloliquefaciens preparation for controlling viral diseases of plants and application thereof
  • Bacillus amyloliquefaciens preparation for controlling viral diseases of plants and application thereof
  • Bacillus amyloliquefaciens preparation for controlling viral diseases of plants and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1 prevents and treats the screening of TMV antagonistic bacteria Ba33 bacterial strain

[0018] Pick the purified strain and inoculate it in NB liquid medium, keep the constant temperature at 28°C, and shake at 140rpm for 48 hours. Take 20ml and mix it with an equal volume of 80 times TMV juice for 15 minutes, then rub the TMV-inoculated rotten spot host Sansheng NN tobacco, and inoculate the upper part of each strain with 2 For a single leaf, use sterile water and TMV as a control, and investigate the rot inhibition rate for 3 to 5 days. The strains with antagonistic activity in the preliminary test were tested repeatedly in the same way, with 3 strains for each treatment, and three repetitions. The results are shown in Table 1, figure 1 shown.

[0019] Table 1 Inhibitory effect of different strains on tobacco common mosaic virus

[0020]

[0021] The investigation of keratotic spot inhibition rate shows: bacterial strain Ba33 has the best inhibitory e...

Embodiment 2

[0022] The configuration of embodiment 2 anti-plant virus disease Ba preparations

[0023] Powder flow chart: Activation of Ba33 strains, preparation of culture solution --- seed liquid inoculation --- cell fermentation 3 times --- mixing with filler diatomaceous earth --- spray drying to remove water --- raw cell powder- --Adding filler---thalline preparation (1 billion / gram). In the obtained bacterial cell preparation, the mass of the filler diatomite accounts for 8% of the total mass of the preparation.

[0024] The Ba preparation was diluted 150 times, mixed with 80 times TMV juice in equal volume for 15 minutes, rubbed and inoculated with Sansheng NN tobacco, and inoculated the upper 2 leaves of each plant. The mixture of sterile water and TMV was used as a control, and cultured with Ba33 medium and NB at the same time. Base instead of Ba preparation solution for comparison. Calculate the scab inhibition rate.

[0025] Table 2 Effects of different diatomite additions o...

Embodiment 3B

[0028] The inhibitory effect of embodiment 3Ba preparation on TMV

[0029] For Sansheng NN tobacco at the 5-6 leaf stage, do the following treatment: ① 150-fold dilution of foliar spray preparation, 10ml per plant, 24 hours later, inoculate 160-fold TMV juice; ② Mix 80-fold TMV juice with an equal volume of the preparation, Friction inoculation; ③ Inoculation with 160 times TMV juice, 24 hours later, spray the preparation on the leaves, 10ml per plant. For each treatment of 3 plants above, the upper 3 leaves were inoculated. Respectively, NB medium and water were used instead of Ba preparation solution as control CK. The average number of dead spots was counted. The results are shown in Table 3, figure 2 shown.

[0030] Table 3 The mode of action of the Ba preparation of biocontrol bacteria on TMV (average number of dead spots)

[0031]

[0032]

[0033] It can be seen from Table 3 that the number of dead spots in the three modes of action of the biocontrol agent B...

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Abstract

The invention discloses a Bacillus amyloliquefaciens preparation for controlling viral diseases of plants, and the preparation provided by the invention contains the following ingredients: a Bacillus amyloliquefaciens culture liquid and diatomite, wherein the viable count of Bacillus amyloliquefaciens in the Bacillus amyloliquefaciens culture liquid is 100 billion cfu / mL; the weight of the diatomite accounts for 8% of the total weight of the preparation; and the strain, named as Bacillus amyloliquefaciens, is preserved in the China general microbiological culture collection center (CGMCC) on April 23th, 2010, and the collection number is CGMCC No.4127. The Bacillus amyloliquefaciens preparation for controlling the viral diseases of plants has the capabilities of inactivating virus particles, inhibiting reproduction and proliferation of viruses and inducing host resistance; and the control efficiency is more than 80% in a green house and 49.09% in the field. The Bacillus amyloliquefaciens preparation has high application values for controlling viral diseases of crops (especially tobacco crops).

Description

technical field [0001] The invention relates to an anti-plant virus disease Ba preparation and its application. Background technique [0002] Viral diseases are one of the important diseases of crops. At present, the control effects of anti-plant virus agents are far from meeting the needs of agricultural production, and there is an urgent need to develop new anti-virus agents. Although chemical pesticides are still the mainstay, from the perspective of sustainable development, the development of bio-derived antiviral agents that can produce high efficiency and high activity is the key to realizing industrialized biological control. [0003] Chinese literature "Research on the inhibition of tobacco mosaic virus RNA accumulation in leaves by biocontrol bacteria Ba33," Plant Protection, 2010, 36(3): 82-84." Action, Journal of Jilin Agricultural University, 2010, 32(4): 383-386." It is recorded that Bacillus amylobacter Ba33 has an inhibitory effect on tobacco mosaic virus (T...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01N25/22A01P1/00A01P21/00
Inventor 王凤龙申莉莉马强杨金广王志华钱玉梅刘常荣陈丹孙惠青董小卫李晓周显升刘伟卜晓东赵砚棠刘宝平朱战营耿宝峰
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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