Platycarya strobilacea sieb infructescence polyphenols extract as well as extracting method and application thereof

The invention relates to a technology of Fructus fragrans fruit and an extraction method, which is applied in the field of polyphenol extracts of medicinal active components of Fructus Radix et Rhizoma and its extraction, and can solve the problems of high extraction temperature, long extraction time, low polyphenol purity, and the like, Achieve the effect of low extraction temperature, good inhibition effect and simple extraction process

Inactive Publication Date: 2011-09-14
NORTHWEST UNIV(CN)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The inventor has used conventional methods to study the extraction and purification technology of polyphenols in the infructuration of the fragrant tree. This method has the disadvantages of long extraction time, high extraction temperature, low extraction rate, and low purity of polyphenols.

Method used

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  • Platycarya strobilacea sieb infructescence polyphenols extract as well as extracting method and application thereof
  • Platycarya strobilacea sieb infructescence polyphenols extract as well as extracting method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Dry 10kg of the raw material Cinnamomum infructome in the shade until the moisture is 7%, rinse with running water for 3 minutes, remove impurities and sediment, drain the residual water, and crush to 3-5mm. Add 50L of 60% ethanol aqueous solution at 45°C, ultrasonic frequency 30KHz, ultrasonic intensity 10W / cm 2 After extracting for 1 hour, centrifuge the solid-liquid obtained by centrifuging the extract, discard the solid, keep the supernatant, and concentrate the supernatant to 10L at 5-7kPa. The concentrated solution was extracted twice with ethyl acetate, and the organic solvent was evaporated to obtain crude polyphenols. Crude polyphenols were dissolved in 60% ethanol, and were adsorbed by LS-46 macroporous adsorption resin at a sample concentration of 5g / L and a flow rate of 3BV / h. Using 70% ethanol as the eluent, eluted and separated at a flow rate of 4BV. After ethanol is recovered from the eluate under reduced pressure, the polyphenol extract is obtained, wh...

Embodiment 2

[0026] Dry 25kg of the raw material Cinnamomum infructome in the shade until the water content is 8%, rinse with running water for 3 minutes, remove impurities and silt, drain the residual water, and crush to 3-5mm. Add 200L of 50% ethanol aqueous solution at 40°C, ultrasonic frequency 50KHz, ultrasonic intensity 50W / cm 2 Extract for 1 hour, centrifuge the solid-liquid obtained by centrifuging the extract, discard the solid, keep the supernatant, and concentrate the supernatant to 40L at 5-7kPa. The concentrated solution was extracted three times with ethyl acetate, and the organic solvent was evaporated to obtain crude polyphenols. Crude polyphenols were dissolved in 60% ethanol, and were adsorbed by LS-46 macroporous adsorption resin at a sample concentration of 8g / L and a flow rate of 3BV / h. Using 75% ethanol as the eluent, eluted and separated at a flow rate of 4BV. After ethanol is recovered from the eluate under reduced pressure, the polyphenol extract is obtained, whi...

Embodiment 3

[0029] Dry 15kg of raw material Cinnamomum infructome in the shade until the water content is 8%, rinse with running water for 3 minutes, remove impurities and sediment, drain the residual water, and crush to 3-5mm. Add 80L of 40% ethanol aqueous solution at 40°C, ultrasonic frequency 45KHz, ultrasonic intensity 5W / cm 2After extracting for 1 hour, centrifuge the solid-liquid obtained by centrifuging the extract, discard the solid, keep the supernatant, and concentrate the supernatant to 20L at 5-7kPa. The concentrated solution was extracted once with ethyl acetate, and the organic solvent was evaporated to obtain crude polyphenols. Crude polyphenols were dissolved in 40% ethanol, and were adsorbed by LS-46 macroporous adsorption resin at a sample concentration of 7g / L and a flow rate of 4BV / h. Using 80% ethanol as the eluent, eluted and separated at a flow rate of 5BV. After ethanol is recovered from the eluate under reduced pressure, the polyphenol extract is obtained, whic...

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Abstract

The invention discloses a method for extracting a platycarya strobilacea sieb infructescence polyphenols compound. The method comprises the steps of firstly smashing platycarya strobilacea sieb infructescence, subjecting the platycarya strobilacea sieb infructescence and 40-60wt% ethanol water, in the mass-to-volume ratio of 1: (5-8), to ultrasonic intensified extracting at a temperature of 40-50 DEG C, subjecting the extract to solid-liquid separation, concentrating liquid supernatant to a volume accounting for 1/5-1/3 of an original volume, extracting the concentrated solution by utilizing ethyl acetate, volatilizing the ethyl acetate to obtain crude polyphenol, eluting, separating and purifying the crude polyphenol through an LS-46 macroporous absorption resin, decompressing the eluent and recovering ethanol to obtain a phenols compound extract, and drying and smashing to obtain a product, wherein through spectrophotometry, the polyphenolic content of the product is detected to be more than 70%. In the invention, the extracting temperature is low, the time is short and the energy consumption is low; after being separated and purified by utilizing the LS-46 macroporous absorption resin, the polyphenolic content in the obtained product is more than 70% and the product can be used for preparing drugs for treating nasosinusitis.

Description

technical field [0001] The invention relates to an extract of plant active ingredients and an extraction method, in particular to an extract of polyphenols, an active medicinal ingredient of the inflorescences of chrysanthemum, and an extraction method thereof. Background technique [0002] Platycarya Strobilacea (Platycarya Strobilacea Sieb. et Zucc.) belongs to the plant kingdom Seed Plant Phylum Angiosperm Subphylum Dicotyledonous Plant Class Hamamelis Subclass Juglandaceae Juglandaceae. Huaxiang tree is widely distributed in my country and is produced in the Yellow River Basin and the south of the region, such as: Jiangxi, Hubei, Anhui, Henan, Guangdong, Guangxi, Jiangsu, Zhejiang, Yunnan, Guizhou and other places. It also grows in Korea and Japan. At present, the only single-leaved chrysanthemum of Juglandaceae and Juglandaceae in the world is found in Guangxi, which not only has medicinal value, but also has academic value in plant evolution system. The infructescence...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/52A61P31/04A61P11/02A61K131/00
CPCY02A50/30
Inventor 李冬李稳宏刘庆超李珍延绥宏
Owner NORTHWEST UNIV(CN)
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