Colloidal gold detecting method and device based on digital image processing

A detection method and digital image technology, which is applied in the field of human body fluid immune detection, can solve the problems of being mixed in the instrument, the light is not uniform enough, and quantitative identification cannot be carried out, so as to achieve the effect of ensuring uniformity, luminous intensity, and stability

Active Publication Date: 2011-09-14
SHANGHAI UPPER BIO TECH PHARMA
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Problems solved by technology

At present, a gold standard spot method quantitative reader (U2 instrument) can measure HCG. Since the detector used in the U2 instrument is a single-point photodiode, it can only mix the signal of the area to be detected in the instrument. ; In addition, the light source it uses is also a single LED, so the light irradiated on the reaction plate is not uniform enough and the luminou

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  • Colloidal gold detecting method and device based on digital image processing
  • Colloidal gold detecting method and device based on digital image processing
  • Colloidal gold detecting method and device based on digital image processing

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[0026] Below in conjunction with specific embodiment, further illustrate the present invention. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. In addition, it should be understood that after reading the teachings of the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms also fall within the scope defined by the appended claims of the present application.

[0027] The present invention relates to a colloidal gold detection method based on digital image processing. After performing processing operations such as gray scale conversion, edge extraction, and background suppression on the collected digital image, the method finally quantitatively calculates the concentration result corresponding to the sample. Using this method can not only realize the qualitative judgment of the negati...

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Abstract

The invention relates to a colloidal gold detecting method based on digital image processing, comprising the following steps of: collecting RGB (Red, Green, Blue) coloured images by an area array CCD (Charge Coupled Device) detector, carrying out gray level conversion, edge extraction, background suppression and the like on the collected coloured images, and finally quantitatively calculating the corresponding concentration result of a sample. The invention also relates to a colloidal gold detecting device based on the digital image processing; and the device can be used for detecting the colloidal gold by the method provided by the invention so that the device not only realizes the negative and positive qualitative judgement of the sample but also realizes the quantitative application on the lower end.

Description

technical field [0001] The invention relates to the technical field of human body fluid immune detection, in particular to a colloidal gold detection method based on digital image processing and a device thereof. Background technique [0002] Human body fluids contain important information about human health, including the level of various hormones, changes in specific protein concentrations, the presence and amount of pathogens and their corresponding antibodies, etc. Most of these items to be tested can be immuno-infiltrated with colloidal gold Technology (gold standard spot method) to detect, the test result is to observe whether there are red spots on the reaction plate, the depth of the red spots is proportional to the concentration of the analyte, but it can only be qualitatively interpreted by eyes alone, but cannot be quantified. [0003] The gold standard spot method has been widely used in the determination of human chorionic gonadotropin (HCG) in serum or urine. ...

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Application Information

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IPC IPC(8): G01N21/75
Inventor 张健徐建新
Owner SHANGHAI UPPER BIO TECH PHARMA
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