Method for detecting 33 kinds of narcotic drugs in urine by liquid chromatography-tandem mass spectrometry

Abstract

The invention discloses a method for detecting 33 kinds of narcotic drugs in urine by liquid chromatography-tandem mass spectrometry, which is characterized by comprising the following steps: carrying out extraction and purification on samples by using a liquid-liquid extraction method, adding a sodium hydroxide solution into the obtained object so as to realize albuminous degeneration and pH-value adjustment of the obtained object, and adding ethyl acetate into the obtained mixture so as to carry out extraction on the obtained mixture through liquid-liquid extraction, then obtaining to-be-tested samples; carrying out determination on the to-be-tested samples by using a liquid chromatography-tandem quadrupole mass spectrometer, wherein, except for that barbiturates in the to-be-tested samples are analyzed in a negative ion mode, the other compounds in the to-be-tested samples are analyzed in a positive ion mode; and through taking the relative retention time of each narcotic drug and the abundance ratio of a monitoring ion pair of each narcotic drug as the qualitative basis, calculating the residual quantity of each narcotic drug in the samples according to the peak area or peak height of each narcotic drug. By using the method disclosed by the invention, 33 kinds of narcotic drugs can be analyzed at one time, and the test cost is reduced, meanwhile, the method is strong in pertinence, simple in operation, fast in detection speed, wide in detection surface, and easy to popularize and apply. The method can be used in the quick detection and positive result confirmation of entry-exit inspection and quarantine bureaus, disease control centers and public security departments.

Description

technical field [0001] The invention relates to a drug detection method, in particular to a liquid chromatography-tandem mass spectrometry detection method suitable for 33 kinds of drugs in urine. Background technique [0002] Drug abuse and drug trafficking have become a global social hazard. Once a person is infected with drugs, he will often indulge in the deformed "pleasure" imagined by drugs, and have no intention of working and studying. Drug use can also cause a series of family and social problems. In view of the serious harm of drug use to the family, especially to the society, some foreign countries, in addition to taking corresponding management measures for their own drug users, also require applicants to provide proof of non-drug use, such as Russia. As the process of global economic integration accelerates, it is inevitable that other countries will introduce similar regulations for immigrants one after another. In order to actively respond to the requiremen...

AI Technical Summary

Problems solved by technology

At present, the inspection and quarantine department mainly uses colloidal gold technology to quickly detect morphine and methamphetamine in the urine of entry-exit personnel. The false positive rate is extremely high, and only two kinds of drugs can be detected.

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