Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

SCAR marker of tetranychus truncates ehara, specific PCR detection method and kit of tetranychus truncates ehara

A detection method and specific technology, applied in the field of identification and detection of plant pest mites, can solve the problems of unidentified species or dominant species, etc., and achieve the effect of easy to be mastered and accurate identification

Inactive Publication Date: 2011-10-19
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Studies have shown that the main types of spider mites in vegetable fields in northern China are Tetranychus truncatus, Tetranychus urticae and Tetranychus cinnabarinus, among which Tetranychus truncation and Tetranychus cinnabarinus are both red in shape, and Tetranychus urticae is usually yellow-green in shape. When these three kinds of spider mites are mixed together, it is basically impossible to identify their species or dominant species with the naked eye

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • SCAR marker of tetranychus truncates ehara, specific PCR detection method and kit of tetranychus truncates ehara
  • SCAR marker of tetranychus truncates ehara, specific PCR detection method and kit of tetranychus truncates ehara
  • SCAR marker of tetranychus truncates ehara, specific PCR detection method and kit of tetranychus truncates ehara

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Acquisition of the SCAR marker of Tetranychus truncation and primers

[0033]Materials and methods

[0034] 1. Materials

[0035] 1.1 Tetranychus tested

[0036] Tetranychus truncatus was provided by the Plant Protection Institute of Fujian Academy of Agricultural Sciences and has been kept indoors until now; Tetranychus cinnabarinus and Tetranychus urticae were provided by Nanjing Agricultural University. The morphological characteristics of male external genitalia and female genitalia of these three species were observed under a traditional microscope. Adult mites are identified by their color and other morphological characteristics.

[0037] The control species of allopatric spider mites was Hawthorn Tetranychus that occurred on fruit trees, provided by the Fruit Tree Institute of Shandong Academy of Agricultural Sciences.

[0038] The above-mentioned test materials are kept in our laboratory and can be distributed to the public for verification tests. ...

Embodiment 2

[0061] PCR amplification was carried out on the populations of Tetranychus truncatus kept in the room. Using Tetranychus urticae, Tetranychus cinnabarinus and Tetranychus hawthorn as control populations, it was found that only one specific band of 303bp appeared in the population of Tetranychus truncatus. After recovery, cloning and sequencing, the result was consistent with the sequence of the expected fragment (303bp). However, no bands appeared in the other three species as controls, see figure 2 , indicating that the specific primer has strong species specificity for Tetranychus truncation, and is suitable for species identification of Tetranychus truncation. Example 2. Verification of the repeatability, sensitivity and specificity of the detection method

[0062] Intraspecies validation

[0063] Professor Tetsuo Gotoh, a well-known expert on the taxonomy of spider mites from Ibaraki University in Japan, conducted traditional morphological identification of the spider m...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a sequence characterized amplified region (SCAR) marker of tetranychus truncates ehara, a specific PCR detection method and a kit of tetranychus truncates ehara. The SCAR marked nucleotide sequence is as set forth in SEQ ID NO.1. The specific PCR detection method is characterized by that: a specific primer used in the detection is designed by the SCAR marked sequence, and a target amplified region is on the SCAR marked sequence. The specific PCR detection method and the kit of tetranychus truncates ehara provided by the invention have the advantages of strong specificity, high detection efficiency, good operability and the like, thus being capable of being widely used as a low-cost detection technology of tetranychus truncates ehara.

Description

technical field [0001] The invention relates to identification and detection technology of plant pest mite, in particular to SCAR marker and specific PCR detection method and kit of Tetranychus truncation. Background technique [0002] Tetranychus truncation belongs to Arachnidae, Acariidae, Tetranychidae, commonly known as spider mite, and is an important pest in agricultural production. It has a wide range of hosts and mainly damages vegetables, cotton, wheat and other important crops. Tetranychus truncatus causes adult mites and young nymphs to gather on the back of plant leaves for piercing and sucking, causing chlorotic spots on the leaves. In severe cases, it will appear like fire, the leaves will dry up and fall off, and even the plants will die in pieces. [0003] The female adult mite of Tetranychus truncation is pear-shaped, 0.5mm in size, and the body is reddish brown or rusty red; the male adult mite is about 0.3mm in size, and the end of the abdomen is slightly ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 王少丽戴宇婷张友军徐宝云吴青君
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com