Quick virus enrichment method and enrichment device for environmental water sample

Abstract

The invention relates to a quick virus enrichment method and a quick virus enrichment device for an environmental water sample. The sample solution is separated into permeating solution and concentrated solution by adopting direct current filtration and laminar separation enrichment technology; and after the concentrated solution is circularly concentrated, the big water sample is concentrated into a small concentrate sample and then can be used for laboratory detection. The device comprises primary and secondary filter assemblies, a laminar enrichment assembly, a peristaltic pump and a programmable logic controller (PLC) and the like, wherein the secondary filter assembly has back flushing function. The cleaning problem of an internal pipeline is taken into full consideration; and a filter membrane and a filter element are disposable, so cross contamination of samples and growth and propagation of microbes in the pipeline can be avoided. The method and the device are particularly suitable for concentrating the big water sample into the small concentrate sample on site for laboratory detection.

Description

technical field [0001] The invention belongs to the fields of biological analysis testing and environmental science, and in particular relates to a rapid virus enrichment method and an enrichment device for environmental water samples. Background technique [0002] The safety of microbial indicators is the primary problem that threatens the safety of drinking water. The survey results show that the microbial safety of drinking water in my country has not been effectively guaranteed, and the phenomenon of excessive microorganisms is common. Judging from the incidence of water pollution diseases in my country in recent years, the epidemics of aseptic encephalitis, hand, foot and mouth disease, acute hemorrhagic conjunctivitis and neonatal acute myocarditis caused by enteroviruses are on the rise. Viruses are far more resistant to conventional drinking water disinfection methods than bacteria. Even if there is a detectable virus unit in drinking water, it may cause human infect...

AI Technical Summary

Problems solved by technology

However, the above-mentioned enrichment method is only suitable for the separation of substances in relatively clean liquids, and is not suitable for the isolation of viruses from environmental water samples containing more large-diameter particles such as sediment particles, humus, and plankton. When the laminar flow separation and enrichment device separates viruses from the large-volume environmental water sample, its filter membrane will be blocked quickly

[0008] To sum up, for large-volume (above 40L) environmental water samples, there is currently no effective rapid virus enrichment method, let alone a rapid enrichment device available.

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