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Totally-enclosed cell and cell factor preparation device and preparation method thereof

A cytokine, fully enclosed technology, applied in the field of fully enclosed cells and cytokine preparation devices, can solve the problems of low platelet and mononuclear cell enrichment rate, easy contamination by pathogenic microorganisms, and easy mixing of red blood cells, etc. Safety and practicability, easy separation, high enrichment effect

Active Publication Date: 2011-11-16
ZHEJIANG XINGYUE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to overcome the shortcomings of the existing manual cell and cytokine preparation devices and methods, such as complex operation, large blood loss, low enrichment rate of platelets and mononuclear cells, easy mixing of red blood cells and easy contamination by pathogenic microorganisms, the present invention provides a Fully enclosed cell and cytokine preparation device and preparation method thereof with simple and convenient operation, low loss of platelets and mononuclear cells, high enrichment rate, and significant safety and practicability

Method used

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  • Totally-enclosed cell and cell factor preparation device and preparation method thereof
  • Totally-enclosed cell and cell factor preparation device and preparation method thereof
  • Totally-enclosed cell and cell factor preparation device and preparation method thereof

Examples

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Embodiment 1

[0028] refer to figure 1 , 2

[0029] A fully enclosed cell and cytokine preparation device, comprising an upper blood bag 4, a lower blood bag 6 and a transparent connecting thin tube 5 connecting the upper and lower blood bags 4, 6, the upper blood bag 4 is provided with an anticoagulation inlet 1, A plasma suction port 21 for pumping plasma and an output port 22 for outputting platelet-rich plasma or mononuclear cells;

[0030] The bottom of the upper blood bag 4 is conical, the top of the lower blood bag 6 is conical, and the upper blood bag 4, the connecting thin tube 5 and the lower blood bag 6 form a dumbbell-shaped blood collection bag.

[0031] The outer cover of the blood collection bag is connected with a hard tube cover 7, the bottom of the upper cylinder of the tube cover is provided with a tapered blood bag bracket, and the middle part is provided with a connecting thin tube 5 and an upper blood bag 4. The window 8 at the bottom, the blood collection bag is e...

Embodiment 2

[0039] refer to figure 1 , 2 , detailing the method for preparing platelet-rich plasma using the fully enclosed cell and cytokine preparation device of the present invention:

[0040] The method for preparing platelet-rich plasma using the fully enclosed cell and cytokine preparation device comprises the following steps:

[0041] 1) Import the anticoagulated blood, bone marrow or umbilical cord blood into the blood collection bag;

[0042] 2), set up the blood collection bag on the hard sleeve 7, wrap the blood collection bag with the hard sleeve 7, put it into the centrifuge, and start the centrifuge;

[0043] 4), cut off the lower blood bag 6 and the connecting thin tube 5;

[0044] 5) Fully mix the plasma in the upper blood bag 4, extract the plasma in the upper blood bag 4 to test the platelet concentration; save the plasma in the upper blood bag 4 for future use.

[0045] In step 2), observe whether the interface between buffy coat and blood plasma is located in the c...

Embodiment 3

[0052] refer to figure 1 , 2 , detailing the method for preparing mononuclear cells using the fully enclosed cell and cytokine preparation device of the present invention:

[0053] The method for preparing mononuclear cells using the fully enclosed cell and cytokine preparation device includes the following steps:

[0054] 1) Import the anticoagulated blood, bone marrow or umbilical cord blood into the blood collection bag;

[0055] 2), erect the blood collection bag on the hard sleeve 7, wrap the blood collection bag with the hard sleeve 7, put it into the centrifuge, and start the centrifuge;

[0056] 4), cut off the lower blood bag 6 and the connecting thin tube 5;

[0057] 5) Fully mix the plasma and blood cells in the upper blood bag 4, wrap the blood collection bag with the hard tube sleeve 7 and put it into the centrifuge, start the centrifuge, and centrifuge at a low speed.

[0058] 6) Discard all the plasma and save the precipitated cells for future use.

[0059]...

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Abstract

The invention discloses a totally-enclosed cell and cell factor preparation device which comprises an upper blood bag, a lower blood bag and a thin connecting pipe, wherein the upper blood bag is provided with an anti-coagulation inlet, a plasma sucking port for sucking plasma and an output port for outputting cells and cell factors; the bottom of the upper blood bag is conical; the top of the lower blood bag is conical; and the upper blood bag, the thin connecting pipe and the lower blood bag are formed into a dumbbell-shaped blood collecting bag. The cell and cell factor preparation method comprises the following steps of: introducing anti-coagulation blood, marrow and cord blood into the blood collecting bag, erecting the blood collecting bag on a hard sleeve, putting the hard sleeve into a centrifuge, and starting the centrifuge; separating the lower blood bag from the thin connecting pipe; fully mixing the plasma in the upper blood bag, and extracting the plasma in the upper blood bag for testing platelet concentration; reserving the plasma in the upper blood bag for later use; or centrifuging again to extract a plasma layer, and reserving centrifugally precipitated cells. The invention has the advantages of simplicity and convenience in operation, low loss of platelets, mononuclear cells and cell factors and high enrichment ratio, and obviously improves the safety and practicality.

Description

technical field [0001] The invention relates to a fully enclosed cell and cytokine preparation device and a preparation method thereof. technical background [0002] Blood (including blood obtained from different sources or body parts, such as umbilical cord blood, peripheral blood, central blood vessel and cardiac puncture blood, etc.) and bone marrow contain red blood cells, white blood cells, stem cells, platelets, plasma and other components. Among them, white blood cells can be divided into mononuclear white blood cells and multinuclear white blood cells, which are an important part of the human immune system. Stem cells are mononuclear cells, and their size and intracellular granule content are similar to mononuclear leukocytes. Platelets and plasma contain a large amount of cytokines, and these two components are usually concentrated and called platelet-rich plasma. These different components play different therapeutic roles in different diseases. Therefore, isolat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12N5/078C07K14/52C07K1/14A61M1/00
Inventor 柳华邹晓晖
Owner ZHEJIANG XINGYUE BIOTECH
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