Method for growing cell embryos of Dendrobium huoshanense

A technology of somatic embryos and Dendrobium Huoshanense, which is applied in the field of plant tissue culture, can solve the problems of inconsistent developmental stages of somatic embryos, low survival rate of regenerated plants, and large size differences, so as to achieve effective protection, medicinal value, and operation The effect of simple process

Inactive Publication Date: 2012-10-03
芜湖华信生物药业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In order to solve the problems existing in the existing Dendrobium Huoshanense somatic embryogenesis technology that the induction of callus requires exogenous hormones, the developmental stages of somatic embryos are inconsistent, the synchronization is small, and the size difference is large, which leads to the low survival rate of regenerated plants. , a method for somatic embryogenesis of Dendrobium Huoshanense provided by the invention

Method used

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  • Method for growing cell embryos of Dendrobium huoshanense
  • Method for growing cell embryos of Dendrobium huoshanense
  • Method for growing cell embryos of Dendrobium huoshanense

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Embodiment 1

[0027] A method for somatic embryogenesis of Dendrobium Huoshanense comprises the following steps:

[0028] Step 1, induction of sterile test-tube plantlets

[0029] Mature fruits of wild Dendrobium huoshanense produced in Taipingfan Township, Huoshan County, Anhui Province were taken, and the surface was disinfected with 1% sodium hypochlorite aqueous solution for 60 min, and then rinsed with sterile double distilled water for 3 times, and then the fruit was cut open and the seeds were taken out , and the seeds were sprinkled on MS solid medium, and cultivated for 7 months at a temperature of 22 ± 2°C, a light intensity of 2000 lux, and a daily light of 12 hours to obtain 4 cm long sterile test-tube plantlets of Dendrobium huoshanense, see figure 1 .

[0030] Step 2, induction of embryogenic callus

[0031] Take the morphological lower end of the nodal stem section of sterile test-tube plantlets, insert it into a solid permeable medium with a controlled oxidation-reduction...

Embodiment 2

[0037] A method for somatic embryogenesis of Dendrobium Huoshanense comprises the following steps:

[0038] Step 1. Induction of sterile test-tube seedlings: take the mature fruit of wild Dendrobium huoshanense produced in Taipingfan Township, Huoshan County, Anhui Province, first disinfect the surface with 1% sodium hypochlorite aqueous solution for 60 minutes, and then rinse with sterile double distilled water for 5 minutes. Then cut the fruit, take out the seeds, sprinkle the seeds on MS solid medium, and cultivate them for 7 months at a temperature of 22±2°C, a light intensity of 2500 lux, and 12 hours of light per day to obtain 5 cm long Huoshan Dendrobium sterile test-tube seedlings.

[0039] Step 2, induction of embryogenic callus: Take the morphological lower end of the nodal stem segment of the sterile test tube plantlet, insert it into a solid permeable medium with a controlled redox potential of -0.03 volts, and place it at a temperature of 22±2°C 1. Induced and cu...

Embodiment 3

[0043] A method for somatic embryogenesis of Dendrobium Huoshanense comprises the following steps:

[0044] Step 1. Induction of sterile test-tube seedlings: take the mature fruit of wild Dendrobium huoshanense produced in Taipingfan Township, Huoshan County, Anhui Province, first disinfect the surface with 1% sodium hypochlorite aqueous solution for 60 min, and then rinse with sterile double distilled water for 4 Then cut the fruit, take out the seeds, sprinkle the seeds on MS solid medium, and cultivate them for 6 months at a temperature of 22±2°C, a light intensity of 3000 lux, and 12 hours of light per day to obtain 6 cm long Huoshan Dendrobium sterile test-tube seedlings.

[0045] Step 2. Induction of embryogenic callus: take the morphological lower end of the nodal stem segment of the sterile test-tube plantlet, insert it into a solid permeable medium with a controlled redox potential of -0.02 volts, and place it at a temperature of 22±2°C , Induced and cultured in the ...

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Abstract

The invention relates to a method for growing cell embryos of Dendrobium huoshanense, which comprises the following operating steps: (1) inducing sterile in-vitro cuttings; (2) inducing embryonic calli; (3) inducing somatic embryos; and (4) performing synchronous propagation of somatic embryos to obtain a large amount of cell embryos of Dendrobium huoshanense. In the method, morphological lower ends of the jointed stem segments of the sterile in-vitro cuttings are used as explants, the oxidation-reduction potential of the induction culture medium is controlled, exogenous hormone is not required to be added to induce the embryonic calli of Dendrobium huoshanensem, and the exogenous hormone is prevented from being enriched into complete plants through somatic cells and endangering health ofhuman body. By controlling ambient humidity, embryonic calla are grown into somatic embryos, the consistency of spherical cell embryos is over 90 percent, and the survival rate of regenerated plants is over 85 percent. In the invention, the process is simple and not limited by time and seasons, and industrial automatic production of g cell embryos of Dendrobium huoshanense and in-vitro cuttings of Dendrobium huoshanense can be carried out in door for satisfying needs for production and planting.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for somatic embryogenesis of Dendrobium huoshanense. Background technique [0002] Huoshan Dendrobium ( Dendrobium huoshanense C. Z. Tang et S. J. Cheng), also known as Huo Dendrobium, Huo Huo and Mi Hu, belongs to the genus of Dendrobium orchidaceae. The top grade of dendrobium is deeply favored by modern people. However, the growth of Huoshan Shijie has extremely strict environmental requirements. In addition to the collection of medicinal farmers in the past dynasties and the changes in the modern ecological environment, the wild Huoshan Shijie resources are on the verge of extinction. Now only a very small amount is transplanted in the Changchong Medicinal Plant of Huoshan County. Although Dendrobium Huoshanense has many flowers, it bears few fruits, and the seed embryos of Dendrobium Huoshanense are undifferentiated and have no endosperm,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 朱慧秋
Owner 芜湖华信生物药业股份有限公司
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