Giant salamander oligosaccharide peptide, preparation method and application thereof in cosmetics

Abstract

The invention discloses giant salamander oligosaccharide peptide, a preparation method and application thereof in cosmetics, wherein the molecular weight of the prepared giant salamander oligosaccharide peptide is smaller than 4000Da, a sugar chain is connected with serine or threonine through GalNAc, and the giant salamander oligosaccharide peptide has a -Galbeta1-3GalNAcalpha-O-Ser / Thr structure. The preparation method comprises the following steps of: homogenizing giant salamander grume at the temperature of 0-10 DEG C; adding 1-3 time volume of 0.01-0.1M phosphate buffer and complex enzyme into a homogenate and carrying out enzymolysis for 12-48 hours, wherein the pH value of the phosphate buffer is 7-8, the mass ratio of the complex enzyme to the homogenate is 0.1-1 percent, and the complex enzyme is formed by proportionally mixing ocean alkali protease, papain, pepsin and trypsinase in the activity unit ratio of 3:3:2:2; centrifugalizing to obtain a supernatant, separating by adopting an ultrafiltration membrane separator with the interception molecular weight of below 4000Da and obtaining a liquid permeating an ultrafiltration membrane; collecting an OD280nm absorption peak through a Sephadex LH-20 molecular sieve chromatography column; and freezing and drying the collected liquid after passing through macroporous absorbent resin and activated carbon to prepare the giant salamander oligosaccharide peptide.

Description

Technical field:

[0001] The invention relates to an oligosaccharide peptide prepared from giant salamander (Andrias davidianus) mucus, a preparation method and its application in cosmetics. Background technique:

[0002] The giant salamander (Andrias davidianus) is a rare and special product unique to my country, and it is a second-class protected animal in the country. It is mainly distributed in streams in the middle and upper reaches of the Yangtze River, the middle and upper reaches of the Pearl River and the upper reaches of the Han River. Since the 1990s, Hunan, Hubei, Shaanxi and Guangxi have established a large number of giant salamander domestication and breeding enterprises. In Hunan Province alone, there are about 35 giant salamander domestication and breeding enterprises, with an annual reproductive capacity of 40,000 to 50,000. With the development of breeding scale, the intensive processing and utilization of giant salamander resources has become an urgent iss...

Examples

Embodiment 1

[0018] a. Collect the giant salamander mucus, and homogenize the giant salamander mucus with a homogenizer at 0-10°C;

[0019] b. Add 1 to 3 times the volume of 0.01 to 0.1M phosphate buffer solution with a pH of 7 to 8 and a compound enzyme with a mass ratio of 0.1 to 1% to the homogenate, and perform enzymatic hydrolysis for 12 to 48 hours; The compound enzyme is to mix marine alkaline protease, papain, pepsin and trypsin according to the activity unit ratio of 3:3:2:2;

[0020] c. Centrifuge to get the supernatant, use an ultrafiltration membrane separator with a molecular weight cut-off of 4000Da to separate, and get the liquid that passes through the ultrafiltration membrane;

[0021] d. Collect OD by Sephadex LH-20 molecular sieve chromatography column (2.5×60~100cm) 280nm absorption peak;

[0022] e. The collected liquid is desalinated by macroporous adsorption resin (HPD series) and decolorized by activated carbon, and then freeze-dried to prepare giant salamander ol...

Technical field:

[0001] The invention relates to an oligosaccharide peptide prepared from giant salamander (Andrias davidianus) mucus, a preparation method and its application in cosmetics. Background technique:

[0002] The giant salamander (Andrias davidianus) is a rare and special product unique to my country, and it is a second-class protected animal in the country. It is mainly distributed in streams in the middle and upper reaches of the Yangtze River, the middle and upper reaches of the Pearl River and the upper reaches of the Han River. Since the 1990s, Hunan, Hubei, Shaanxi and Guangxi have established a large number of giant salamander domestication and breeding enterprises. In Hunan Province alone, there are about 35 giant salamander domestication and breeding enterprises, with an annual reproductive capacity of 40,000 to 50,000. With the development of breeding scale, the intensive processing and utilization of giant salamander resources has become an urgent iss...

Examples

Embodiment 1

[0018] a. Collect the giant salamander mucus, and homogenize the giant salamander mucus with a homogenizer at 0-10°C;

[0019] b. Add 1 to 3 times the volume of 0.01 to 0.1M phosphate buffer solution with a pH of 7 to 8 and a compound enzyme with a mass ratio of 0.1 to 1% to the homogenate, and perform enzymatic hydrolysis for 12 to 48 hours; The compound enzyme is to mix marine alkaline protease, papain, pepsin and trypsin according to the activity unit ratio of 3:3:2:2;

[0020] c. Centrifuge to get the supernatant, use an ultrafiltration membrane separator with a molecular weight cut-off of 4000Da to separate, and get the liquid that passes through the ultrafiltration membrane;

[0021] d. Collect OD by Sephadex LH-20 molecular sieve chromatography column (2.5×60~100cm) 280nm absorption peak;

[0022] e. The collected liquid is desalinated by macroporous adsorption resin (HPD series) and decolorized by activated carbon, and then freeze-dried to prepare giant salamander ol...

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