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Bacillus pumilus preparation for processing tobacco stems

A technology for microbial preparations and bacteria, applied in the field of microbial preparations, can solve the problems that have not yet been discovered, and achieve the effects of reducing irritation, reducing the concentration and energy of smoke, and increasing the amount of aroma.

Inactive Publication Date: 2011-11-23
HONGYUN HONGHE TOBACCO (GRP) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After literature search, no report with the same content as this discovery has been found

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] ——Test tube slant culture

[0026] A Luria-Bertani (LB) medium was used, and the formula was: 10 g of peptone, 10 g of sodium chloride, 5 g of yeast extract, 20 g of agar, and distilled water to a volume of 1000 ml. Sterilize at 121°C for 25 minutes and place on a slope. Inoculate Van35, culture at 28°C for 24 hours, and obtain test tube seeds.

Embodiment 2

[0028] - Seed cultivation

[0029] A Luria-Bertani (LB) medium was used, and the formula was: 10 g of peptone, 10 g of sodium chloride, 5 g of yeast extract, and distilled water to a volume of 1000 ml. Sterilize at 121°C for 25 minutes. Inoculate the Van35 test tube species and culture at 28°C for 12 hours to obtain liquid strains.

Embodiment 3

[0031] - Preparation of microbial preparations

[0032] Take the liquid strain, inoculate it into 200ml of LB liquid medium according to the inoculum amount of 0.1%, and cultivate it in a shaker at 37°C with a rotation speed of 180r / min until the OD value = 2.0. Centrifuge at 3500rpm / min for 10min, wash with sterile water Precipitate, centrifuge, and finally shake evenly with 20mL sterile water, and dilute 10 times before use to obtain the bacterial agent.

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PUM

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Abstract

The invention discloses a microorganism preparation for tobaccos as well as a preparation method and application thereof, belonging to the technical field of tobacco additives. A production bacterial strain liquid is fermented and cultured to obtain the corresponding microorganism preparation. The production bacterial strain is bacillus pumilus.Van35, the bacterial strain is collected in the Center for General Microorganism of China Committee for Culture Collection of Microorganisms and the collection number is CGMCC No.3411. The microorganism preparation provided by the invention can obviously improve the qualities of stems, such as fragrance amount, joviality and the like; impure gas, irritation and the like are reduced; smoke concentration and strength are reduced and remaining taste becomes more comfortable so that the doping amount of the stems in a cigarette is further increased so that aims of reducing harm, decreasing coke content and reducing the production cost of the cigarette are achieved.

Description

technical field [0001] The invention belongs to the technical field of cigarette additives, and more specifically, the invention relates to a microbial preparation. At the same time, it also relates to the preparation method of the microbial preparation and its application in the tobacco industry. Background technique [0002] With the improvement of my country's cigarette technology level and the continuous development of harm reduction and tar reduction in the tobacco industry, expanded shredded stems are more widely used in cigarette formulations. After adding expanded shredded stems to the formula, due to its high filling value, the amount of shredded tobacco in cigarettes can be reduced, which not only saves the cost of cigarettes, but also reduces the amount of tar in cigarettes. [0003] However, the current technical level of comprehensive utilization of tobacco stem resources is relatively backward, the waste of idle tobacco stems is large, the blending ratio of sh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A24B15/20C12R1/07
Inventor 段焰青者为王明锋陈兴王家俊王素娟范多青陈玉芸王庆华
Owner HONGYUN HONGHE TOBACCO (GRP) CO LTD
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