Preparation method of immunosensor sensitive membrane for detecting antigen

Abstract

The invention discloses a preparation method of an immunosensor sensitive membrane for detecting antigens. It oxidizes the dried substrate by immersing it in piranha lotion until hydroxyl groups are evenly distributed on the surface of the substrate, and then reacts the hydroxyl groups on the surface of the substrate with the hydrolyzed 3-aminopropyltriethoxysilane until 3-aminopropyltriethoxysilane nanoparticles are evenly distributed on the surface of the substrate, and then the 3-aminopropyltriethoxysilane nanoparticles on the surface of the substrate are combined with protein A or protein G, and then passed on the above substrate The antibody corresponding to the antigen to be detected is dropped on the surface, so that protein A or protein G can bind to the antibody. The method of the present invention overcomes the shortcomings of weak connection of antibody molecules, effectively maintains the activity of antibody reactive molecules, and utilizes the specific orientation of protein A and protein G to improve the effectiveness of antibody-antigen binding by effectively controlling the consistency of antibody orientation. Thereby increasing the sensitivity of the sensor.

Description

technical field [0001] The invention relates to a preparation method of an immunosensor sensitive film for detecting antigens, and belongs to the fields of medical testing instruments and environmental monitoring instruments. Background technique [0002] The existing medical detection methods for antigens are expensive, complex, time-consuming, not easy to carry, and the cost of a single detection is high. It is inevitable to provide a low-cost, convenient and fast high-sensitivity antigen immunosensing detection method. A biosensor is an analysis and detection device made by combining biological sensitive elements with physical elements. Its basic principle is to convert the specific reaction and signal of the biological sensitive element into optical, electrical , sound and other easy-to-detect signals, so as to know the relevant signals of the object under test. Immunosensors use the high specific binding ability between antigens and antibodies to immobilize antibodies...

AI Technical Summary

Problems solved by technology

But now the sensor still has unresolved defects such as loose fixation and poor reactivity.

For example, the preparation method of chemically modified sensitive membrane will destroy the antibody activity and reduce the defect of detection sensitivity; while the preparation method of biologically modified sensitive membrane, the antibody is not fixed firmly and the film formation is unstable

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