A kind of stage-by-stage solid culture method of Beauveria bassiana

A technology for solid culture of Beauveria bassiana, applied in the direction of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems that hinder the production and application of Beauveria bassiana insecticides, control effects, and unstable product quality , high temperature and humidity requirements, to overcome the uncontrollable humidity, to meet the needs of mycelium growth, and to achieve the effect of low production cost

Inactive Publication Date: 2011-12-21
FUJIAN ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the solid-state fermentation stage, most manufacturers in my country currently adopt open production modes such as shallow trays and screen windows. The production process has high requirements for temperatur

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0037] Example one:

[0038] 1 The cultivation of liquid strains includes the following steps:

[0039] 1.1 Strain: Beauveria bassiana BbCeFZ01 strain (Fujian Forestry Science and Technology, 2006, 33(2): 129-131.), preserved by the Forest Protection Research Institute of Fujian Academy of Forestry, is a well-known strain.

[0040] 1.2 Slope strains: Preliminarily subculture and activate the Beauveria bassiana strains with test tube slant medium at 25°C. It can be used when the slant surface is full of spores. The slant medium is SDAY medium.

[0041] 1.3 Liquid culture medium: prepare 2L of SDY culture medium, fill it in 500ml Erlenmeyer flasks, each with 200ml culture medium, sterilize by steam and high temperature at 121℃ for 30min, cool it for later use.

[0042] 1.4 Inoculation: In a sterile operating table, pick a small amount of spores and lawns from the bacterial seed slope and connect them to the above liquid culture medium.

[0043] 1.5 Shaker culture: shake culture in a const...

Example Embodiment

[0052] Embodiment two:

[0053] 1 The cultivation of liquid strains includes the following steps:

[0054] 1.1 Strain: Beauveria bassiana BbSMYXTR03 strain (Fujian Forestry Science and Technology, 2011, 38 (2): 10-13), preserved by the Forest Protection Research Institute of Fujian Academy of Forestry, is a well-known strain.

[0055] 1.2 Inclined strains: the same as in Example 1.

[0056] 1.3 Liquid culture medium: same as Example 1, a total of 4L was prepared.

[0057] 1.4 Inoculation: in the same example one.

[0058] 1.5 Shaker culture: the same as in Example 1, except that the shaker culture time is 60h.

[0059] 2 Solid culture, including the following steps:

[0060] 2.1 Solid culture medium formula and preparation:

[0061] The formula of Beauveria bassiana solid culture material: 10.7kg of wheat bran, 3.5kg of chaff, 12.4L of water.

[0062] Preparation method: same as Example 1.

[0063] 2.2 Inoculation: Same as Example 1, except that the inoculation amount is 15%.

[0064] 2.3 Myce...

Example Embodiment

[0067] Embodiment three:

[0068] 1 The cultivation of liquid strains includes the following steps:

[0069] 1.1 Strain: Beauveria bassiana B08 (Chinese Forest Pests and Diseases, 2008, 27(2): 1-3, 18.) strain, preserved by the Forest Protection Research Institute of Fujian Academy of Forestry, is a well-known strain.

[0070] 1.2 Inclined strains: the same as in Example 1.

[0071] 1.3 Liquid culture medium: same as in Example 1, a total of 8L was prepared.

[0072] 1.4 Inoculation: in the same example one.

[0073] 1.5 Shaker culture: the same as in Example 1, except that the shaker culture time is 60h.

[0074] 2 Solid culture, including the following steps:

[0075] 2.1 Solid culture medium formula and preparation:

[0076] The formula of Beauveria bassiana solid culture material: 20kg of wheat bran, 5kg of bagasse, 25L of water.

[0077] Preparation method: same as Example 1.

[0078] 2.2 Inoculation: Same as Example 1.

[0079] 2.3 Mycelium growth stage: same as in Example 1.

[0080] 2.4...

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PUM

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Abstract

The invention provides a method for carrying out solid culturing on beauveria bassiana by stages, which is characterized in that the beauveria bassiana solid culturing process is divided into two stages, i.e. a mycelium growing stage and a sporulating stage; in the mycelium growing stage, a closed fermenting mode is adopted and a liquid strain is inoculated with a solid culture material in a closed container to carry out culturing; and in the sporulating stage, the solid culture material on which mycelia full grow is taken out in a buckled mode and an open fermenting mode is adopted. The method for carrying out solid culture on the bassiana, which is provided by the invention, has simple process and does not need complex equipment. The fermenting period of the method is shortened to 6 to 7 d from 10 to 14 days in the prior art. The production efficiency is greatly improved. Meanwhile, the requirement on the environment humidity is low. The probability of the mixed fungi pollution is obviously reduced. The production cost is low. The method is easy to control and has high success rate. The product quality is stable. The method can be applied to laboratory production, can be used asthe preferred scheme of upgrading of technology of masses of small and medium bassiana manufacturers and has obvious social and economic benefits.

Description

technical field [0001] The invention relates to a solid culture method for fungi, in particular to a Beauveria bassiana Beauveria bassiana (hereinafter referred to as Beauveria bassiana) solid culture method, which belongs to the field of biological fermentation methods. Background technique [0002] Beauveria bassiana is a kind of entomopathogenic fungi with a wide range of hosts for agricultural and forestry pests and strong pathogenicity. It plays a pivotal role in the research and application of pest biological control. Because Beauveria bassiana is harmless to the environment and vertebrates, easy to cultivate, wide insecticidal spectrum, and strong pathogenicity, it is currently the most widely used fungal insecticide, and has achieved great success in the biological control of various agricultural and forestry pests. Obvious results have been achieved. [0003] At present, the production of Beauveria bassiana at home and abroad mainly adopts the liquid-solid ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
Inventor 何学友蔡守平黄金水熊瑜杨希
Owner FUJIAN ACAD OF FORESTRY
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