Volatile organic compounds as diagnostic markers in the breath for lung cancer

一种传感器阵列、纳米颗粒的技术，应用在呼气分析仪领域，能够解决尚未得到满足等问题

Active Publication Date: 2012-01-18
TECHNION RES & DEV FOUND LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] There is an unmet need for sensor arrays based on rapid response of multiple sensors that provide improved sensitivity as well as improved selectivity to specific VOCs indicative of cancer

Method used

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  • Volatile organic compounds as diagnostic markers in the breath for lung cancer
  • Volatile organic compounds as diagnostic markers in the breath for lung cancer
  • Volatile organic compounds as diagnostic markers in the breath for lung cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0122] Example 1: Collection of exhaled breath

[0123] After deep exhalation, the subject inhales to the total lung capacity through a mouthpiece containing a sleeve on the breathing port to remove more than 99.99% of the VOC environmental pollutants from the air inhaled during inhalation. Then the subject held a 10-15cm H 2 Exhale at O ​​pressure to ensure that the parchment paper is sealed to eliminate nasal mist. Collect the exhaled gas through the separate exhalation port of the mouthpiece. 2 Gas-purified non-reactive polyester film gas sampling bag (purchased from Eco Medics). The exhaled breath of each subject was analyzed at least five times. A total of 90 breath samples were used, of which 26 were obtained from healthy individuals and 64 were obtained from lung cancer patients. First, use various diagnostic methods, including bronchoscopy biopsy, computed tomography (CT) scan, and lung puncture to make clinical diagnosis of the patient. None of the lung cancer patien...

Embodiment 2

[0124] Example 2: Analysis of exhaled breath by GC-MS

[0125] The exhaled breath samples from subjects with lung cancer and from healthy individuals were collected in a polyester film sample bag and gas chromatography-mass spectrometry (GC-MS) combined with solid phase microextraction (SPME) was used. )analysis. SPME technology is used to pre-concentrate VOCs in breath samples. Will have been 1) polydimethylsiloxane (PDMS), 2) polydimethylsiloxane-divinylbenzene (PDMS / DVB) or 3) polydimethylsiloxane-Carboxen (PDMS / Carboxen) (purchased from Sigma-Aldrich) was inserted into a polyester film bag with a manual SPME handle coated with extracted fibers. Will be at 500cm 3 Up to 1,000cm 3 Each breath sample in between was concentrated by the SPME method for an extraction time of 2 hours, and each breath sample was delivered to the GC-MS using a manual SPME handle. Insert the extracted fiber in the manual SPME handle into the GC syringe operated in splitless mode. The oven tempera...

Embodiment 3

[0131] Example 3: Synthesis and coating of gold nanoparticles

[0132] Use a variety of C 4 -C 18 The chain length of alkanethiolates and ω-functionalized alkanethiolates (such as 11-mercapto-1-undecol) coat gold nanoparticles with an average size of about 5 nm. The standard two-stage method for gold synthesis according to Brust et al. (J. Chem. Soc., Chem. Com., 801, 1994, 2) was used with some modifications according to Hostetler et al. (Langmuir, 1998, 14, 24) Nano particles. Briefly, first pass the phase transfer reagent TOAB (80ml, 34.3mM) to the AuCl 4 - From HAuCl 4 ·XH 2 The O aqueous solution (25ml, 31.5mM) was transferred to the toluene solution. After separating the organic phase, excess mercaptan is added to the solution. According to the type of thiol, make thiol: HAuCl 4 ·XH 2 The molar ratio of O was varied from 1:1 to 10:1 to prepare a monodispersed solution of gold nanoparticles with an average size of 5 nm. For example, for gold nanoparticles with an average...

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Abstract

Sensor array for detecting biomarkers for cancer in breath samples. The sensor array is based on 2D films or 3D assemblies of conductive nanoparticles capped with an organic coating wherein the nanoparticles are characterized by a narrow size distribution. Methods of use of the sensor array for discriminating between patterns of volatile organic compounds from healthy individuals and patients with various types of cancer are disclosed.

Description

Invention field [0001] The present invention relates to a breath analyzer including a sensor array of conductive nanoparticles coated with an organic coating for detecting cancer. Background of the invention [0002] Breath analysis has always been regarded as a reliable technique for diagnosing certain medical conditions by detecting volatile organic compounds (VOC) in exhaled breath. Diagnosis is usually performed by collecting a breath sample into a container and then measuring the specific VOC using mass spectrometry. [0003] The composition of the VOC in the exhaled breath depends on the metabolic process of the cell, and it includes saturated and unsaturated hydrocarbons, oxygen-containing compounds, sulfur-containing compounds, and nitrogen-containing compounds, among others. In healthy individuals, composition provides clear chemical characteristics, and there is relatively narrow variability between samples of a single individual and samples of different individuals. [0...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/497
CPCG01N33/0034G01N33/574G01N33/0047G01N33/57423B82Y15/00G01N2033/4975
Inventor 霍萨姆・海克彭刚欧娜・亚当斯
Owner TECHNION RES & DEV FOUND LTD
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