Production method of cylindrotheca clostetium

A production method, the technology of Cylindrical algae, applied in the production field of Cylindrical algae, can solve problems such as lack of establishment, difficulty in cultivating successful Cylindrical algae, difficulty in cultivating Cylindrical algae to meet the requirements, etc., to achieve increased growth speed, increased growth efficiency, and fast growth Effect

Active Publication Date: 2012-02-22
QINGDAO QIHAO NUTRITION TECH
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Problems solved by technology

The cultivation of Cylindrical algae has not been established, whether it is laboratory cultivation or productive cultivation, especially its continuous cultivation method is pioneering, and there is no similar technology in China for comparison
In the actual production and scientific research process, Cylindrophyta is a benthic algae with large individuals, so it is easy to settle at the bottom of seawater, and in its life stag

Method used

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  • Production method of cylindrotheca clostetium
  • Production method of cylindrotheca clostetium

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[0041] Example

[0042] Take Cylindrotheca sp. (MACC / B169) as an example. Before the production of Cylindrotheca sp. (MACC / B169), it is necessary to expand and cultivate the pure Cylindrotheca sp. to obtain a cylindrical column with higher purity and better quality. As the algae species for production and cultivation, the expansion method is as follows:

[0043] First, prepare the trace element solution. From zinc sulfate (ZnSO 4 ·4H 2 O) 23 mg, copper sulfate (CuSO 4 ·5H 2 O) 10 mg, manganese chloride (MnCl 2 ·4H 2 O) 178 mg, iron citrate (FeC 6 H 5 O 7 ·5H 2 O) 3.9 grams, sodium molybdate (NaMoO 4 ·2H 2 O) 7.3 mg, sodium ethylene diammonium tetraacetate (Na 2 EDTA) 4.35 grams, cobalt chloride (CoCl 2 ·6H 2 O) 12 mg, dissolved in 1000 ml of pure water.

[0044] Then prepare the vitamin solution. From 0.5 mg of vitamin B 12 , And 100 mg of vitamin B 1 Dissolved into 1000 ml of pure water.

[0045] Then prepare the basic solution. From sodium nitrate (NaNO 3 ) 74.8 mg, sodium dihydr...

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Abstract

A production method of cylindrotheca clostetium comprises the following steps: respectively connecting a culture medium charging system, a material liquid collecting system, and an air supply system with a hanging-bag photo bioreactor so as to form a culture system of cylindrotheca clostetium; adding cylindrotheca clostetium after expansion culture and the culture medium into the reactor; continuously charging the culture medium with a constant flow rate and continuously injecting air; during the culture process, controlling the seawater temperature to be 10 DEG C-35 DEG C, the salinity to be20-30, and the illuminance to be 3000-10000 lx, controlling the pH of the culture medium to be within 7.8-8.4; continuously collecting mature cylindrotheca clostetium under the action of continuous air injection. The invention not only establishes a mature cylindrotheca clostetium expansion culture method, but also establishes a fully-closed continuous culture method of cylindrotheca clostetium based on the established expansion culture method, and has the advantages that the method accords with the physiological characteristics and ecological habits of cylindrotheca clostetium; during cylindrotheca clostetium culture, the whole culture process is clean, continuous, controllable, has high culture efficiency, and is applicable to the characteristics of production application.

Description

technical field [0001] The invention relates to a production method of Cylindrical algae, which is suitable for cultivating and collecting Cylindrical algae in production. Background technique [0002] Cylindrotheca (Cylindrotheca sp.) taxonomic status as diatoms, diatoms pinnata, phyllophytes, rhizophyceae, Cylindrotheca genus. The cells are long, with a round end surface and elongated ends, in the shape of a mouth or a head. The cells are 69.5 microns long and 4.5-5 microns wide. There are 2 or more pigment bodies, located in the main body of the cell, not deep into the mouth. The total lipid content of Cylindrical sp. accounts for 16% to 37% of the dry weight, and the EPA content accounts for 3.36% to 15.9% of the total fatty acids. It has high nutritional value and is suitable for being used as bait organisms such as sea cucumbers and abalones, and can also be used as production organisms. Raw material for diesel. Cylindrical sp. grows in the natural environment and ...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
Inventor 宫庆礼邓志科赫勇周丽
Owner QINGDAO QIHAO NUTRITION TECH
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