High efficiency liquid chromatography detection method for content of raddeanin A in rhizoma anemones raddeanae

A technique of high performance liquid chromatography and cylindronate, which is applied in the field of detection of the two ends of traditional Chinese medicine, can solve the problems of complicated operation, low sensitivity, poor reproducibility, etc., and achieves the effects of simple operation method, simple method and saving detection cost.

Inactive Publication Date: 2012-03-21
仲崇琳
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  • Abstract
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Problems solved by technology

[0004] The invention provides a high-performance liquid chromatography detection method for the content of cyperone A in the traditional Chinese medicine two-end point to solve the problems of complex operation, poor reproducibility and low sensitivity in the prior art. The method is suitable for the two-head point medicinal material , Quality control of two sharp preparations

Method used

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  • High efficiency liquid chromatography detection method for content of raddeanin A in rhizoma anemones raddeanae
  • High efficiency liquid chromatography detection method for content of raddeanin A in rhizoma anemones raddeanae
  • High efficiency liquid chromatography detection method for content of raddeanin A in rhizoma anemones raddeanae

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Effect test

Embodiment 1

[0019] (1) Chromatographic conditions and system suitability test: as filler; mobile phase: acetonitrile-water 80:20; detection by evaporative light scattering detector; flow rate: 0.2ml / min; drift tube temperature detected by evaporative light scattering detector : 60°C, air flow rate: 1.0L / min.

[0020] (2) Preparation of reference substance solution: accurately weigh out an appropriate amount of bamboo joint A reference substance, add methanol to make a solution containing 0.4mg per 1ml, and get it;

[0021] (3) Preparation of the test solution: Take 2g of the sharp powder at both ends, place it in a conical flask with stopper, add 50ml of methanol, close it tightly, weigh it, ultrasonically treat it for 0.5 hours, let it cool, and weigh it again. Add methanol to make up the lost weight, shake well, and filter; measure 10ml of the continued filtrate, evaporate to dryness in a water bath, add methanol to the residue to dissolve, transfer to a 10ml volumetric flask and dilute to t...

Embodiment 2

[0024] (1) Chromatographic conditions and system suitability test: octadecylsilane-bonded silica gel as filler; mobile phase: acetonitrile-water 86:14; detection by evaporative light scattering detector; flow rate: 0.6ml / min; evaporation Drift tube temperature detected by light scattering detector: 90°C, air flow rate: 2.0 L / min.

[0025] (2) Preparation of reference substance solution: accurately weigh out an appropriate amount of bamboo joint A reference substance, add methanol to make a solution containing 0.4mg per 1ml, and get it;

[0026] (3) Preparation of the test solution: Take 5g of the sharp powder at both ends, place it in an Erlenmeyer flask with stopper, add 50ml of methanol, close it tightly, weigh it, ultrasonically treat it for 0.8 hours, let it cool, and weigh it again. Add methanol to make up the lost weight, shake well, and filter; measure 10ml of the continued filtrate, evaporate to dryness in a water bath, add methanol to the residue to dissolve, transfer to a...

Embodiment 3

[0029] (1) Chromatographic conditions and system suitability test: octadecylsilane bonded silica gel as filler; mobile phase: acetonitrile-water 93:7; detection by evaporative light scattering detector; flow rate: 1ml / min; evaporating light The drift tube temperature detected by the scattering detector: 130°C, and the air flow rate: 3.1 L / min.

[0030] (2) Preparation of reference substance solution: accurately weigh out an appropriate amount of bamboo joint A reference substance, add methanol to make a solution containing 0.4mg per 1ml, and get it;

[0031] (3) Preparation of the test solution: Take 8g of the sharp powder at both ends, place it in a stoppered Erlenmeyer flask, add 50ml of methanol, close it tightly, weigh it, ultrasonically treat it for 1 hour, let it cool, and weigh it again. Add methanol to make up the lost weight, shake well, and filter; measure 10ml of the continued filtrate, evaporate to dryness in a water bath, add methanol to the residue to dissolve, transf...

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Abstract

The invention relates to a high efficiency liquid chromatography detection method for the content of raddeanin A in rhizoma anemones raddeanae, belonging to a detection method of rhizoma anemones raddeanae. In the method, an evaporative light-scattering detector is adopted, an external standard two-point method is used for quantifying, octadecylsilane bonded silica is taken as a filler, a mixed solution of methanol or acetonitrile and water is taken as a flowing phase, and the flow speed of the flowing phase is 0.2-1 ml per minute; and a methanol ultrasonic treatment method is adopted for sample pretreatment. The method has the advantages of high sensitivity, stability, high reproducibility and capability of effectively controlling the qualities of the rhizoma anemones raddeanae and a preparation thereof.

Description

Invention field [0001] The invention relates to a method for detecting two-headed tip of traditional Chinese medicine, in particular to a method for detecting content of two-headed tip of traditional Chinese medicine and its preparation. Background technique [0002] The two-pointed tip is also known as the bamboo joint of Xiangfu, which is the dry rhizome of Anemone raddeana Regel, a plant of the Ranunculaceae family. Mainly distributed in the three northeastern provinces and Shandong, Hebei, Shanxi and other places, Jilin Province has the most output. The medicinal materials are mostly excavated in summer, and the residual stems and fibrous roots are removed and dried. This medicine was first published in the Essentials of Materia Medica by Liu Wentai in the Ming Dynasty. It has the effect of "eliminating wind, dampness, and carbuncle". As early as the Qing Dynasty, Wang Xugao's "Ding Ai San" used two tips to treat breast cancer. It is now clinically used for wind-cold damp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/71G01N30/02G01N30/06A61P29/00A61P35/00A61P19/02A61P17/00A61K125/00
Inventor 仲崇琳杨美林于江波
Owner 仲崇琳
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