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Composition comprising the purified extract of bee venom for preventing and treating degenerative brain diseases

A technology of degenerative encephalopathy and extracts, applied in the direction of drug combination, medical raw materials derived from arthropods, drug delivery, etc., can solve the therapeutic effect or improvement effect of unpurified bee venom extracts on brain degenerative diseases, report or publish, etc. question

Active Publication Date: 2012-04-04
洪思有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, there is no report or disclosure about the therapeutic effect or improvement effect of the purified bee venom extract on brain degenerative diseases in any of the above cited documents, the disclosure of which is incorporated herein by reference

Method used

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  • Composition comprising the purified extract of bee venom for preventing and treating degenerative brain diseases
  • Composition comprising the purified extract of bee venom for preventing and treating degenerative brain diseases
  • Composition comprising the purified extract of bee venom for preventing and treating degenerative brain diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Example 1: Preparation of crudely purified extract of bee venom

[0066] 10.0 g of dried bee venom collected from honeybees was dissolved in water, and impurities were removed by filtration using a syringe filter (Minisart RC 15, 0.20 micron, Sartorius Co. Germany). The filtrate was dried with a lyophilizer (FDCF-12012, Operon Co. Korea) to obtain 9.76 g of dried crude purified extract of meli venom (hereinafter designated as "HP-01"). The dry powder was used as a test sample in the following experiments.

Embodiment 2

[0067] Example 2: Preparation of Purified Extract of Bee Venom Using Gel Filtration Chromatography

[0068] 100 mg of the dry crude purified extract prepared in Example 1 was dissolved in 1.0 ml of distilled water (HPLC grade) and subjected to gel filtration chromatography according to the conditions disclosed in Table 1 to obtain 20 fractions. Each fraction was added to a protein dialysis membrane (Spectra / por 7, Spectrum Co. USA) and the membrane was immersed in a cylindrical glass flask containing 500 ml of distilled water (HPLC grade) for dialysis with stirring for 90 minutes. After completion of the dialysis desalination process, the desalted solution present in the membrane was lyophilized using a lyophilizer (FDCF-12012, Operon Co. Korea) for 3 days and each of the purified fractions was collected to obtain 74 mg of bee venom purified extract (yield : 74%) (hereinafter designated as "HP-01G").

[0069] Table 1

[0070]

[0071] The compositions of HP-01 and HP-01G ...

Embodiment 3

[0075] Example 3: Preparation of a purified extract of bee venom using the salting-out method

[0076] 100 mg of the dry crude purified extract prepared in Example 1 was dissolved in 5.0 ml of distilled water (HPLC grade) to adjust to 20 mg / ml, and the solution was subjected to a salting process at room temperature by adding ammonium sulfate dropwise with stirring for 1 hour to form a 30% ammonium sulfate solution. The solution was further stirred at room temperature for 1 hour and subjected to a salting-out process by dropwise addition of ammonium sulfate to form an 80% solution.

[0077] The solution was left alone at 0° C. for 2 hours to provide sufficient time for the sufficient salting-out process, and centrifuged at 15000 rpm for 15 minutes by using an ultracentrifuge (Ultra 5.0, Hanil Science Medical Co. Ltd, Korea). The supernatant was collected and the pellet was dissolved in 5 ml of distilled water (HPLC grade) to desalt and lyophilize each to give 19 mg of a purifi...

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Abstract

The present invention relates to a composition comprising the purified extract of bee venom for preventing and treating degenerative brain diseases and the use thereby. The purified extract of bee venom shows potent inhibitory effect on microglial cell activation and abnormal circling behavior using by animal model of degenerative brain disease as well as potent cell-protective activity therefore, it can be useful in treating and preventing the degenerative brain disease as a medicament.

Description

technical field [0001] The present invention relates to a composition comprising a purified extract of bee venom and its use for the prevention and treatment of degenerative encephalopathy. Background technique [0002] In the twentieth century, as the average life expectancy of human beings has increased with the rapid development of life sciences and medicine, new social problems have emerged including an increasing proportion of the elderly population, especially senile neuronal diseases such as fatal neuronal system dysfunction. Stroke, Alzheimer's disease (AD), Parkinson's disease (PD), etc. have increased. [0003] In particular, Parkinson's disease is caused by the death of neurons in the substantia nigra compact part of the brain, and often produces neuronal disease. In particular, congenital Parkinson's disease accounts for more than 80% of people with Parkinson's disease and is the etiological cause of a severe disorder in the elderly population (Ennio Esposito et...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/64A61P25/28A61P25/16A61K9/08
CPCA61K38/1767A61K31/137A61K38/465A61K9/4858A61K38/12A61K9/2018A61K35/64A61K9/1623A61K31/417A61K9/0019A61K45/06A61P25/00A61P25/16A61P25/28A61K2300/00A61K35/63A61K9/19
Inventor 尹圣泰申大熙林芳虎金载庸崔在锡
Owner 洪思有限公司
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