Ziziphus jujube mill photosphere rhizosphere bacillus sp. capable of promoting growth

A Bacillus subtilis and rhizosphere growth-promoting technology, which is applied in the direction of application, bacteria, and microorganisms, can solve the problems of screening and application of winter jujube rhizosphere growth-promoting bacterial strains that have not been reported, so as to achieve excellent strain resources and promote growth The effect of growth and content increase

Active Publication Date: 2012-04-11
SHANDONG FOREST SCI RES INST +1
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Problems solved by technology

At present, there is no report on the screening and application o

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  • Ziziphus jujube mill photosphere rhizosphere bacillus sp. capable of promoting growth
  • Ziziphus jujube mill photosphere rhizosphere bacillus sp. capable of promoting growth
  • Ziziphus jujube mill photosphere rhizosphere bacillus sp. capable of promoting growth

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Embodiment 1

[0018] (1) Screening process:

[0019] By gradient dilution method, 87 isolates were isolated from rhizosphere soil samples of Dongzao demonstration garden of Binzhou Forestry Bureau, Shandong Province. Purify the isolated bacteria by the three-section line method, and judge whether the strain is purified by microscopic examination, number the purified bacteria, pick a single colony, and transfer it to an LB slant for future use. 23 bacterial strains with better green-preserving effect were screened out by the green-preserving method, combined with the radish cotyledon weight-increasing method, and finally one bacterial strain with good growth-promoting effect was screened out, which was designated as DZ1.

[0020] (2) Colony characteristics and cell morphology:

[0021] After cultured on LB plates for 24 hours, DZ1 was round and opaque with a smooth surface. The cell size of the bacteria is (0.7-0.8) μm×(2.0-3.0) μm. Sporulation, G + , straight rod-shaped cells.

[0022]...

Embodiment 3

[0032] Dongzao rhizosphere growth-promoting Bacillus subtilis DZ1 was shaken and cultured in a reciprocating shaker at 37°C and 180rpm for 2 days, and the concentration of the bacteria adjustment solution was 5.0×10 8 cfu / ml, inoculated into decomposed chicken manure to make winter jujube bio-organic fertilizer (0.5×10 8 cfu / g). Take the same amount of decomposed chicken manure as a control, and apply 8.5kg to each tree. The method of fertilization is furrow fertilization on the southeast, northwest and four sides. The length, width and height of the ditch are 60, 30 and 20 cm respectively. The bio-organic fertilizer of winter jujube is fully mixed with the excavated soil and then backfilled. Every time 30 trees were treated, a protection line was set. After two years of application, the total amount of amino acids in the rhizosphere soil of Dongzao was determined by formaldehyde titration, the total sugar content was determined by anthrone colorimetry, and the organic acid ...

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Abstract

The invention discloses a ziziphus jujube mill rhizosphere bacillus sp. capable of promoting growth, which is named Bacillus sp. by classification, and is preserved in China General Microbiological Culture Collection Center (CGMCC) on 11th, Oct, 2011, the preserved number is CGMCC No.5318. The invention also discloses an application of the ziziphus jujube mill rhizosphere bacillus sp. in growth promotion of ziziphus jujube mill. The bacterial strain is prepared to an inoculant for inoculating the sprouts of ziziphus jujube mill, the result shows that the inoculant is capable of obviously promoting the growth of seedling root of ziziphus jujube mill, raising the proportion of fine roots in root, and increasing the growth of ground diameter and height of the ziziphus jujube mill sprouts. Control groups have obvious differences. The ziziphus jujube mill rhizosphere bacillus sp. capable of promoting growth is inoculated in fermented chicken manure to prepare a biological fertilizer, and is used around the root of seven-year ziziphus jujube mill, thereby, the root vitality is substantially raised, the content of the root secretion in the ziziphus jujube mill rhizosphere soil is increased, and the absorption and utilization capability of the soil nutrient of the ziziphus jujube mill rhizosphere is enhanced.

Description

technical field [0001] The invention relates to a winter jujube rhizosphere growth-promoting Bacillus subtilis and application thereof. Background technique [0002] Winter jujube (Ziziphus jujube Mill.) is a plant of the genus Ziziphus Mill. in the family Rhamnaceae. It is mainly distributed in the Yellow River Delta of my country. It is 10 to 13 times higher than citrus fruits, very rich in nutrition, and has extremely high nutritional and therapeutic value. The production management process of winter jujube is very complicated, and fertilization is one of the very important links. The fertilization effect of winter jujube is not only affected by the soil, the nutritional characteristics of the tree itself, and the amount of fertilizer applied, but also related to the type of fertilizer. At present, the fertilization of winter jujube is mainly based on quick-acting fertilizers, but the root system of winter jujube is underdeveloped, the growth is slow, the number of capil...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F3/02C05F11/08C12R1/125
CPCY02A40/20
Inventor 刘方春杜秉海邢尚军杜振宇姚良同丁延芹马丙尧马海林陈波
Owner SHANDONG FOREST SCI RES INST
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