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Continuous culture for 1,3-propanediol production using high glycerine concentration

A propylene glycol, high-concentration technology, applied in 1 field, can solve problems such as expensive, discontinuous, and unable to give glycerin performance

Inactive Publication Date: 2012-04-18
METABOLIC EXPLORER
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although this process is environmentally friendly, it has the disadvantages of i) the use of vitamin B12, a very expensive cofactor and ii) a discontinuous process due to the instability of the production strain
[0009] However, using 105 g.l in a synthetic feed medium without organic nitrogen -1 The same Clostridium acetobutylicum DG1 pSPD5 strain tested with industrial glycerol did not give the same performance as using pure glycerol at the same concentration (see Example 1)

Method used

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  • Continuous culture for 1,3-propanediol production using high glycerine concentration
  • Continuous culture for 1,3-propanediol production using high glycerine concentration
  • Continuous culture for 1,3-propanediol production using high glycerine concentration

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Embodiment Construction

[0028]The present invention relates to a method for the production of 1,3-propanediol in a continuous fermentation process of glycerol, comprising culturing on a medium a production microorganism allowing the conversion of glycerol to 1,3-propanediol, and Recovery of 1,3-propanediol, characterized in that the medium comprises a high concentration of technical glycerol comprising glycerol, and wherein the producing microorganism is a microorganism pre-adapted to grow in the presence of a high concentration of technical glycerol .

[0029] The term "microorganism" means a microorganism selected from the group consisting of bacteria, yeast and fungi. Preferably, said microorganism is a bacterium preferably selected from the group consisting of Enterobacteriaceae, Bacillaceae, Clostridiaceae, Streptomycetaceae and Corynebacteriaceae. More preferably, the bacteria are selected from the group consisting of Escherichia sp. (preferably Escherichia coli), Klebsiella sp. (preferably Kl...

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Abstract

The present invention concerns a new method for the production of 1,3- propanediol comprising culturing a microorganism on a culture medium with high glycerine content. The invention also concerns a new microorganism, or strain of microorganism, adapted for the production of 1,3-propanediol from medium comprising high glycerine content. The invention also concerns an "adapted microorganism" which glycerol metabolism is directed to 1,3-propanediol production, and which is allowed to grow in the presence of a high concentration of industrial glycerine. The invention also concerns a biosourced 1,3-propanediol obtained by the process thereof. Finally the invention concerns the use of the above described biosourced 1,3- propanediol as extender chain in thermoplastic polyurethane, as monomers in polytrimethylene terephtalate and as a component in cosmetics formulations.

Description

technical field [0001] The present invention relates to a new method for the production of 1,3-propanediol comprising culturing microorganisms on a medium with a high glycerol content. The present invention also relates to novel microorganisms, or strains of microorganisms, adapted for the production of 1,3-propanediol from media comprising a high glycerol content. The invention also relates to "adapted microorganisms" whose glycerol metabolism is directed towards 1,3-propanediol production and which are allowed to grow in the presence of high concentrations of industrial glycerol. The invention also relates to biosourced 1,3-propanediol produced by the method of the invention. Finally, the invention relates to the use of the aforementioned 1,3-propanediol of biological origin as extender chain in thermoplastic polyurethanes, as monomer in polytrimethylene terephthalate and as a component in cosmetic formulations . Background technique [0002] 1,3-Propanediol (PDO) is on...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/18C12N1/36
CPCC12N1/20C12P7/18C12N1/36Y10S435/842C12R2001/145
Inventor M.查蒂奥J-Y.杜博伊斯P.索凯尔
Owner METABOLIC EXPLORER
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