Direct-vat bacillus subtilis starter and preparation method thereof

Abstract

The invention relates to a direct-vat bacillus subtilis starter and a preparation method thereof. The method comprises the following steps of: performing seed culture on bacillus subtilis, performing high-density scale-up culture on the bacillus subtilis, and preparing a bacterium starter by a freeze-drying method to finally obtain the direct-vat bacillus subtilis starter. A culture medium for the seed culture and the high-density scale-up culture of the bacillus subtilis comprises the following components in percentage by weight: 2 percent of sucrose, 1 percent of bean pulp, 0.1 percent of monopotassium phosphate, 0.05 percent of magnesium sulfate and the balance of water, wherein the pH value is 7.0 to 7.2. Concentration conditions for preparing the bacterium starter by the freeze-drying method are that: the centrifugal rotating speed is 5,000r / min, the centrifugal time is 10min, the centrifugal temperature is 4 DEG C, and a cryoprotectant contains 5 percent of maltodextrin, 4 percent of sucrose, 0.8 percent of trehalose and 0.2 percent of tween. The survival rate of the direct-vat bacillus subtilis starter produced by the method is 95.3 percent, the viable count is 2.69*10<10>cfu / g, and ideal liquid and solid fermentation effects can be achieved according to one thousandth of inoculation amount. The preparation method for developing the novel high-activity direct-vat bacillus subtilis starter on the basis of the strain is applied to the liquid-state fermentation and solid-state fermentation of bacillus subtilis-related products.

Description

Technical field [0001] The invention belongs to the field of biological preparations, in particular to a direct cast type Bacillus subtilis starter and a preparation method thereof. Background technique [0002] Direct-input starter refers to a series of highly concentrated and standardized freeze-dried starter strains, which can be directly added to heat-treated raw materials for fermentation, without the need for activation, expansion and other pretreatments. At present, there is no report on the application of direct injection Bacillus subtilis starter at home and abroad. Summary of the invention [0003] The purpose of the present invention is to overcome the shortcomings of the prior art, and provide a direct injection Bacillus subtilis starter with simple operation, high content of live bacteria, high vitality, and small inoculation amount and a preparation method thereof. [0004] The technical scheme for achieving the objective of the present invention is as follows: [0005...

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Problems solved by technology

At present, there is no report on the application of direct-throwing Bacillus subtilis starter at home and abroad

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