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Target fluorescence spectrum unmixing method based on elimination of background fluorescence

A fluorescence elimination and fluorescence spectrum technology, which is applied in the field of target fluorescence spectrum unmixing based on background fluorescence elimination, can solve the problems of not considering background fluorescence contribution, inapplicability, etc., and achieve robustness, good calculation efficiency, and good unmixing effect of effect

Inactive Publication Date: 2012-05-02
SHANGHAI JIAO TONG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Although there are many methods to eliminate background fluorescence, including the method of setting narrow-band filters and the method of using near-infrared target fluorescent agents, these methods have certain inapplicability
The existing fluorescence spectrum unmixing algorithm uses non-automatic target fluorescence area extraction to assist in the spectral unmixing algorithm, but does not take into account the contribution of background fluorescence to the photon counting of the target fluorescence area, and does not take into account the contribution of background fluorescence to the photon counting of the target fluorescence area. Separation of background fluorescence and target fluorescence and unmixing of target fluorescence

Method used

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  • Target fluorescence spectrum unmixing method based on elimination of background fluorescence
  • Target fluorescence spectrum unmixing method based on elimination of background fluorescence
  • Target fluorescence spectrum unmixing method based on elimination of background fluorescence

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Embodiment 1

[0050] The target fluorescence spectrum unmixing method based on background fluorescence elimination of the present invention comprises steps as follows:

[0051] (1) First, the image is preprocessed to remove image noise and enhance the contrast between the target fluorescent area and the background fluorescent area.

[0052] (2) Considering that most of the target fluorescent areas have the characteristics of local cluster aggregation, these aggregation areas are covered and surrounded by the background fluorescent area, which is the main change area of ​​each channel, we use the nuclear maximum autocorrelation factor method to get all The targeted fluorescent region of the channel image.

[0053] (3) After obtaining the target fluorescent area, we set the photon count value of the target fluorescent area in the multi-channel image to zero, and combined with the photon count value of the background fluorescence around the target fluorescent area, use the image repair technol...

Embodiment 2

[0099] The process of the present invention is as figure 1 shown. After preprocessing, the influence of background fluorescence is reduced as much as possible, by automatically marking the main change area of ​​the multi-channel image, that is, the target fluorescence area, combined with zeroing the photon count value of the target area and performing background fluorescence photon counting on this area The smooth patch, so as to get the desired background fluorescence image. On this basis, the alternating least squares method is used to optimize the equation (16) through continuous loop iterations to unmix the target fluorescence until a satisfactory accuracy result is achieved. Below just specific embodiment is described in detail:

[0100] 1. Image preprocessing

[0101] Preprocessing is to eliminate image Poisson noise and some artifacts generated during image acquisition, and at the same time smooth the image to a certain extent, enhance the target fluorescence area, a...

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Abstract

The invention relates to a target fluorescence spectrum unmixing method based on elimination of background fluorescence, comprising the following steps of: (1) carrying out preprocessing on an image; (2) extracting a target fluorescence area; (3) after obtaining the target fluorescence area, setting photon counting values of the target fluorescence area in a multichannel image to be zero; and recycling photon counting values of other background fluorescence around the target fluorescence area to carry out smooth patching of background fluorescence signals on zero-setting areas, and obtaining a background fluorescence image; and (4) utilizing an original image to subtract the background fluorescence image, obtaining the target fluorescence image, carrying out linear unmixing on the obtained target fluorescence image by a linear unmixing algorithm and obtaining a final target fluorescence unmixing result. Compared with the prior art, the target fluorescence spectrum unmixing method has the advantages that good unmixing effect can be obtained still under the condition with sparse channels and the like.

Description

technical field [0001] The invention relates to a technology related to multispectral imaging and image processing, in particular to a target fluorescence spectrum unmixing method based on background fluorescence elimination. Background technique [0002] Spectral unmixing technology is a basic and important work in the field of multispectral imaging. In a multi-spectral imaging system, the photon counts emitted by different single substances are different in different spectral bands, which constitute the emission spectral fingerprint characteristics of a specific single substance, and the emission spectral fingerprint characteristics of these specific substances are called end members. In actual multispectral imaging, the photon counting signals emitted by different substances are mixed and filtered by multiple spectral channels with different central wavelengths before reaching the imaging detection device. The photon count detected by the imaging detector is a mixture of...

Claims

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Application Information

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IPC IPC(8): G06T5/50
Inventor 秦斌杰赵勇汤天衡
Owner SHANGHAI JIAO TONG UNIV