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Method for producing rapamycin fermentation liquid by culturing streptomyces hygroscopicus

A technology of Streptomyces hygroscopicus and rapamycin, which is applied in the field of biomedicine, can solve problems such as unfavorable extraction and purification, low rapamycin fermentation unit, and inability to fully satisfy industrialized production, so as to satisfy industrialized production and be beneficial to extraction. effect of work

Active Publication Date: 2014-12-31
鲁南新时代生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] It can be seen that the current rapamycin bio-fermentation production has a shortcoming in that the rapamycin bio-fermentation unit is not high, which is not conducive to extraction and purification, and cannot fully meet the requirements of industrial production.

Method used

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  • Method for producing rapamycin fermentation liquid by culturing streptomyces hygroscopicus
  • Method for producing rapamycin fermentation liquid by culturing streptomyces hygroscopicus
  • Method for producing rapamycin fermentation liquid by culturing streptomyces hygroscopicus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1100

[0035] The fermentation culture of embodiment 1100L fermentor

[0036] (1) Preparation of culture medium

[0037] The formula of the solid medium is: every liter of the solid medium contains 40g of oatmeal, 20g of glucose, 10g of yeast extract, 2g of calcium carbonate and 16g of agar, and the pH is 7.2. Prepare 0.6L.

[0038] The formula of the seed culture medium is: every liter of the seed culture medium contains 40g of soluble starch, 5g of peptone, 10g of yeast extract, 10g of peanut meal, 10g of glycerin and 2g of calcium carbonate, and the pH is 7.0. Prepare 7L.

[0039] The formula of the fermentation medium is: each liter of the fermentation medium contains 13g of corn starch, 10g of soybean cake powder, 6g of peptone, 35g of glucose, K 2 HPO 4 3g, KH 2 PO 4 3g and NaCL1g, calcium carbonate 3g and foam enemy 3g, the pH is 6.9. Prepare 60L.

[0040] (2) Plate culture of Streptomyces hygroscopicus

[0041] After the solid medium is prepared, put it into an Erlen...

Embodiment 2100

[0053] The fermentation culture of embodiment 2100L fermentor

[0054] (1) Preparation of culture medium

[0055] The formula of the solid medium is: every liter of the solid medium contains 30g of oatmeal, 25g of glucose, 15g of yeast extract, 2g of calcium carbonate and 16g of agar, and the pH is 7.1. Prepare 0.6L.

[0056] The formula of the seed culture medium is: every liter of the seed culture medium contains 30g of soluble starch, 5g of peptone, 7g of yeast extract, 10g of peanut meal, 10g of glycerin, 2g of calcium carbonate, and the pH is 6.9. Prepare 7L.

[0057] The formula of the fermentation medium is: each liter of fermentation medium contains 18g of corn starch, 13g of soybean cake powder, 4g of peptone, 35g of glucose, KH 2 PO 4 4g and NaCL1g, calcium carbonate 3g, foam enemy 4g, pH is 6.5. Prepare 60L.

[0058] (2) Plate culture of Streptomyces hygroscopicus

[0059] After the solid medium is prepared, put it into an Erlenmeyer flask (1x750mL), steriliz...

Embodiment 31000

[0071] The fermentation culture of embodiment 31000L fermentor

[0072] (1) Preparation of culture medium

[0073] The formula of the solid medium is: every liter of the solid medium contains 40g of oatmeal, 20g of glucose, 10g of yeast extract, 2g of calcium carbonate and 16g of agar, and the pH is 7.2. Prepare 1L

[0074] The formula of the seed culture medium is: every liter of the seed culture medium contains 40g of soluble starch, 5g of peptone, 10g of yeast extract, 10g of peanut meal, 10g of glycerin and 2g of calcium carbonate, and the pH is 7.0. Prepare 70L

[0075] The formula of the fermentation medium is: each liter of the fermentation medium contains 13g of corn starch, 10g of soybean cake powder, 6g of peptone, 35g of glucose, K 2 HPO 4 3g, KH 2 PO 4 3g and NaCL1g, calcium carbonate 3g and foam enemy 3g, the pH is 6.9. Prepare 700L

[0076] (2) Plate culture of Streptomyces hygroscopicus

[0077] After the solid medium is prepared, put it into an Erlenme...

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Abstract

The invention discloses a new method for producing rapamycin fermentation liquid by culturing streptomyces hygroscopicus. According to the invention, the fermentation liquid containing rapamycin is prepared by fermentation culture of a microbial strain, namely streptomyces hygroscopicus ACCC No.40417. The rapamycin fermentation liquid prepared by the method disclosed by the invention has high titer, thus being more favorable to extraction and industrialized production.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a method for producing rapamycin by microbial fermentation. Background technique [0002] Rapamycin (RPM, RAPA), also known as Sirolimus, is a triene macrolide isolated from Strepomyces hygroscopicus by the Ayerst Research Institute in Canada in 1975 Antibiotics, it has attracted widespread attention as a new type of highly effective immunosuppressant, and it is also an effective antifungal agent and has antitumor effects. [0003] Rapamycin is a triene macrolide compound composed of a 31-membered ring, which contains a hemicarboxyketone covered by a special α, β-diketopecilyl amine molecule, and its molecular formula is C 51 h 79 o 13 , the molecular weight is 991. Rapamycin is a class of lipophilic antibiotics that usually appear as white crystals. Soluble in methanol, ethanol, acetone, chloroform, ethyl acetate and other organic solvents, almost insoluble ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P17/18C12R1/55
Inventor 赵志全赵丽丽
Owner 鲁南新时代生物技术有限公司
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