A method for cultivating actinomycetes to obtain rapamycin fermentation broth

A technology of actinomycetes and rapamycin, which is applied in the field of fermentation engineering, can solve the problems of unfavorable extraction and purification, can not fully meet the needs of industrial production, and the unit of rapamycin biological fermentation is not high, and achieve the benefit of extraction work Effect
CN103740781BActive Publication Date: 2015-09-09天津北洋百川生物技术有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
天津北洋百川生物技术有限公司
Publication Date
2015-09-09
Patent Text Reader

Abstract

The invention discloses a method for obtaining rapamycin fermentation liquor by utilizing actinoplanes, belonging to the technical field of fermentation engineering. The method comprises the following steps of culturing actinoplanes CGMCC No.8430 on a slant culture medium, inoculating the cultured slant strain onto a shake flask seed culture medium, then inoculating the cultured seed liquor into a seeding tank, and then inoculating the seed liquor into a fermentation culture medium to undergo fermentation culture, thus obtaining the rapamycin fermentation liquor. The method has the beneficial effects that rapamycin with relatively high concentration can be generated from actinoplanes by optimizing the culture medium and controlling the culture conditions; the yield of rapamycin can be about 750mg / L in fermentation experiments in a 30L fermentation tank, and the yield of rapamycin can be about 840mg / L in fermentation experiments in a 200L fermentation tank, so that rapamycin more easily meets the requirement of industrial production; moreover, high concentration is more beneficial for subsequent extraction operation.
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Description

technical field

[0001] The invention belongs to the technical field of fermentation engineering, and in particular relates to a method for obtaining rapamycin fermentation liquid by using actinomycetes. Background technique

[0002] Rapamycin (RPM), also known as sirolimus, is a nitrogen-containing triene macrolide antibiotic with a unique mechanism of action. It has antifungal, antiproliferative and antitumor effects. Rapamycin is a triene macrolide compound composed of a 31-membered ring, containing a hemiketal covered by a special α, β-diketopidine amide molecule, with a molecular formula of C51H79NO13 and a molecular weight of 991, which is White solid crystal, melting point 183-185℃, lipophilic, slightly soluble in water, almost insoluble in ether, but soluble in methanol, ethanol, acetone, chloroform and other organic solvents.

[0003] Rapamycin is currently the most promising new powerful immunosuppressant in the world. Its molecular structure is similar to FK506, b...

Claims

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