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Method for preparing high-activity corn antihypertensive peptide

A blood pressure-lowering peptide, high-activity technology, applied in the field of natural product preparation, to achieve the effect of reducing adverse effects, significant blood pressure lowering effect, and high activity

Inactive Publication Date: 2013-09-04
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are research reports on the hydrolysis of soybean protein isolate by enzymatic membrane reactor method; someone has successfully used the continuous operation of dynamic membrane separation enzyme reactor in the process of biological enzymatic degradation of inulin to produce fructose; but the use of enzymatic hydrolysis-membrane separation Combined technology produces maize hypotensive peptide with high angiotensin-converting enzyme (ACE) inhibitory activity, which has not been reported in the literature so far

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  • Method for preparing high-activity corn antihypertensive peptide
  • Method for preparing high-activity corn antihypertensive peptide
  • Method for preparing high-activity corn antihypertensive peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1, preparation process embodiment

[0040] Experimental setup for hydrolysis of zein in an enzyme membrane reactor such as figure 1 shown.

[0041] Depend on figure 1 , the special device of the present invention is a continuous treatment enzyme membrane reaction system, which includes a reaction tank 1, temperature control system 2, automatic pH titrator 3, strong stirrer 4, feed liquid storage tank 5, peristaltic pump 6, Control valve 7, pressure gauge 8, ultrafiltration membrane assembly 9, pipeline heat exchanger 10, collection tank 11, liquid level controller 12 and nanofiltration membrane assembly 13 constitute, wherein said reaction tank 1 and temperature control system 2, The automatic pH titrator 3 and the strong agitator 4 form a circulating reaction system. The reaction tank 1 is installed at a horizontal position higher than the ultrafiltration membrane module 9 in the installation position, and one end thereof passes through the peristaltic pum...

Embodiment 2

[0064] Embodiment 2 Drying embodiment of corn hypotensive peptide of the present invention

[0065] 1 Experimental method

[0066] 1.1 Freeze-drying of corn hypotensive peptide solution

[0067] The corn hypotensive peptide solution obtained in Example 1 was vacuum-concentrated to 1 / 10 of its original volume at a temperature of 45° C. and a pressure of 0.095 MPa, placed in a stainless steel tray with a liquid level of 2 cm, and pre-cooled in a freeze dryer. After 120 minutes, when the temperature of the material reaches -20°C, it is completely frozen, and the temperature starts to rise. The vacuum degree is 0.040 mbar. The heating temperature starts from -20°C and rises slowly after 18 hours until the temperature reaches 25°C. Freeze dried.

[0068] 1.2 Spray drying of corn hypotensive peptide solution

[0069] The corn peptide solution obtained in Example 1 was vacuum-concentrated to 1 / 5 of its original volume at a temperature of 45° C. and a pressure of 0.095 MPa, and spr...

Embodiment 3

[0079] Example 3 Structural characterization and analysis of the corn hypotensive peptide prepared by the present invention

[0080] 1 Experimental method:

[0081] 1.1 Separation of corn hypotensive peptide by reversed-phase-high performance liquid chromatography (RP-HPLC)

[0082] The corn hypotensive peptide after ultrafiltration is analyzed by RP-HPLC, the conditions are as follows: Agilent 1200 high performance liquid chromatography, ZorbaxSB-C 18 5μm column, ID 4.6×250mm; mobile phase A is pure water + 0.1% trifluoroacetic acid, mobile phase B is acetonitrile + 0.1% trifluoroacetic acid; gradient elution: from 0 to 45min, mobile phase A is from 10% to 35%, the flow rate is 1.0mL / min; the ultraviolet detector detects at 230nm.

[0083] 1.2 High performance liquid chromatography-mass spectrometry / mass spectrometry (HPLC-MS / MS) analysis

[0084] Liquid chromatography conditions: Zorbax SB-C18 5μm column, ID 4.6×250mm; mobile phase A is pure water + 0.1% trifluoroacetic ...

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Abstract

The invention belongs to the technical field of natural product preparation and particularly relates to a method for preparing high-activity corn antihypertensive peptide and a special device. An enzyme membrane reactor comprises a reaction tank, a temperature control system, an automatic pH dripping instrument, a strong force stirrer, a material liquid storage tank, a peristaltic pump, a control valve, a pressure meter, an ultrafiltration membrane assembly, a pipeline heat exchanger, a collection tank, a liquid level controller, a nanofiltration membrane assembly and the like. The process method comprises the following steps that corn gluten meal is subjected to alcohol alkali liquid extraction to obtain concentrated corn protein, the concentrated corn protein is pretreated at high temperature and is then added into a continuous enzyme membrane reactor system, the corn antihypertensive peptide is prepared by adopting the enzyme membrane coupling technology, then, the products are subjected to ultrafiltration grading through the ultrafiltration membrane assembly and then are subjected to desalting through the nanofiltration membrane, and the high-activity corn antihypertensive peptide is obtained. The inhibitory activity of the obtained corn antihypertensive peptide on angiotensin-converting enzyme (ACE) is maintained at a value higher than 86 percent, the protein recovery rate reaches higher than 70 percent, and in addition, the systolic pressure of spontaneously hypertensive rats can be obviously reduced.

Description

technical field [0001] The invention belongs to the technical field of natural product preparation, and specifically relates to a method for preparing corn blood pressure-lowering peptide with angiotensin-converting enzyme (ACE) inhibitory effect by using continuous enzymatic preparation and online multi-stage membrane separation integration technology and a special device thereof. Background technique [0002] my country's annual corn production accounts for 20% of the world's total production, second only to the United States, but my country's corn processing industry mainly uses starch, so only 50% to 60% of corn components are used, and most of the rest are roughage or corn. Treating and discharging them as "three wastes" not only wastes resources, but also easily causes environmental pollution. And contain more than 30%~60% protein in these by-products, these by-products are called corn gluten powder also known as corn yellow powder, how to make full use of the protein r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06
CPCC12M25/02C12M41/44C12M21/18C12M41/40C12M41/12C12M41/26C12M27/02C12M41/18
Inventor 何慧黄文浩吕杰
Owner HUAZHONG AGRI UNIV
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