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Lipidated tumor- associated antigens and immunotherapeutic compositions

A technology of tumor-related antigens and compositions, which can be applied to tumor-specific antigens, tumor rejection antigen precursors, cancer antigen components, etc., and can solve problems such as ineffectiveness

Active Publication Date: 2012-05-30
NAT INST OF HEALTH REPRESENTED BY THE SEC OF THE DEPT OF HEALTH & HUMAN SERVICES NAT INST OF HEALTH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, many of these are not functional

Method used

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  • Lipidated tumor- associated antigens and immunotherapeutic compositions
  • Lipidated tumor- associated antigens and immunotherapeutic compositions
  • Lipidated tumor- associated antigens and immunotherapeutic compositions

Examples

Experimental program
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Embodiment

[0419] Expression of lipidated fusion protein rlipo-E7m

[0420] The E7m gene was obtained using a combinatorial PCR method with overlapping primers. The product of combinatorial PCR was then amplified by conventional PCR (Dillon PJ, Rosen CA. Biotechniques 1990; 9:298, 300). The forward primer used for this step (5'-GGAATTC CATATG CACGGCGATACCCCGACCCTGC-3' (SEQ ID NO: 3)) includes an Nde I cleavage site (underlined), and the reverse primer (5'-AGAGCCG CTCGAG CGGTTTCTGGCTCGCAATCGG-3' (SEQ ID NO: 4)) includes an Xho I cleavage site (underlined). The PCR product was cloned into the expression vector pET-22b(+) (NOVAGEN, Madison, WI) using Nde I and Xho I to obtain pE7m plasmid. As a result, the C-terminus of the recombinant protein contained an additional hexahistidine tag (HisTag).

[0421] The expression plasmid pE7m was transformed into E. coli strain BL21Star(DE3) (INVITROGEN, USA) for protein expression. Transformed cells were incubated overnight at 37°C and then ind...

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Abstract

Disclosed are a novel polypeptide and fusion proteins comprising a first segment having a lipidating sequence and a second segment having the sequence of a tumor-associated antigen The fusion proteins can be lipidated and expressed in bacterial cells Once administered to a subject, said fusion proteins induce immune responses against a tumor.

Description

technical field [0001] The present invention relates to polypeptides and lipidated fusion proteins. The present invention also discloses compositions and methods related to immunotherapy related to these polypeptides and fusion proteins. Background technique [0002] Related applications [0003] The present invention claims priority to U.S. Provisional Application No. 61 / 219,301 filed on June 22, 2009; the content of the earlier application is incorporated herein by reference. Background of the invention [0004] Viral infections can cause a variety of conditions, including cancer. For example, infection by human papillomaviruses (HPVs) causes the development of several cancers, especially cervical cancer, which is the second leading cause of cancer death among women in the world, and it has been conclusively confirmed that HPV is the causative agent of cervical cancer (Schwarz, T.F., Expert Rev Vaccines 7:1465-73, 2008). It is estimated that approximately 493,000 new...

Claims

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Application Information

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IPC IPC(8): C07K14/025A61K39/12A61K39/385A61P31/12A61P35/00C07K14/22C07K19/00C12N15/62
CPCA61K39/0011A61K39/12C07K2319/915C12N2710/20022C07K14/4748C07K14/005A61K38/00C12N7/00A61K2039/55516A61K2039/6018A61K2039/585C12N2710/20034A61P31/12A61P35/00
Inventor 冷治湘陈信伟刘士任庄再成
Owner NAT INST OF HEALTH REPRESENTED BY THE SEC OF THE DEPT OF HEALTH & HUMAN SERVICES NAT INST OF HEALTH
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