Cardiac stent with three-dimensional structure and anisotropy and preparation method thereof

Abstract

The invention relates to a cardiac stent with a three-dimensional structure and anisotropy and a preparation method thereof. According to the cardiac stent, a nanometer fiber stent is weaved by static spinning, a meshed structure is defined by adjusting the orientation, density and length of spinning fibers, suspended nanometer fibers without a substrate allow the attachment and growth of cells, and an excitable mesh is formed by the nanometer fiber stent in a hollow tissue form. The cardiac stent has the advantages that: fiber materials prepared by the method can be used for a system for constructing cardiac tissues in vitro, and the anisotropy of the cells is controlled by aggregating the cells on the nanometer fiber mesh for growing; the nanometer fiber mesh is covered on a support made from polydimethylsiloxane by electric spinning to be suspended in the air; and the three-dimensional structure and the anisotropy can be kept, so that the transmission of nutrient and metabolic products is facilitated by a polymethyl glutarimide fiber-based tissue structure, and an ideal system is provided for tissue engineering. A preparation process is simple and convenient to operate, and has a bright application prospect.

Description

Technical field: [0001] The invention relates to a cardiac nanofiber scaffold and a preparation method thereof, more specifically, a cardiac scaffold with a three-dimensional structure and anisotropy and a preparation method thereof. Background technique: [0002] Tissue engineering is a rapidly growing field that builds on and fuses advances in tissue culture and materials science. Tissue engineering aims to create organ-specific tissue structures for regenerative medicine, such as implantable tissues and organs. An important feature of cardiac tissue is its highly developed structural and functional diversity, and its perfect functional coordination enables cardiomyocytes to act as a pump, which also increases the complexity of cardiac tissue engineering strategies. [0003] The cultivation of cardiomyocyte monolayers is a fundamental approach in regenerative medicine. Attempts to mimic the complexity of natural myocardium by culturing ordered monolayers of anisotropic c...

AI Technical Summary

Problems solved by technology

Clearly, solid substrate-based cardiomyocyte monolayers cannot fully meet these requirements