Cardiac stent with three-dimensional structure and anisotropy and preparation method thereof

An anisotropic, heart stent technology, applied in the direction of stent, spinning solution preparation, fiber treatment, etc., to achieve the effect of maintaining three-dimensional structure and anisotropy, convenient operation, and simple preparation process

Inactive Publication Date: 2012-06-20
WUXI ZHONGKE GUANGYUAN BIOMATERIALS
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  • Abstract
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AI Technical Summary

Problems solved by technology

Clearly, solid substrate-based cardiomyocyte monolayers cannot fully meet these requirements

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Nanofiber scaffolds were prepared as follows:

[0017] (1) Preparation of supporting base: Polydimethylsiloxane (PDMS) was prepared by mixing in a flat petri dish, stored at 80° C. for 5 hours, separated from the petri dish, and washed with ethanol before use.

[0018] (2) Preparation of spinning solution: polymethylglutarimide (PMGI) is dissolved in cyclopentanone and tetrahydrofurfuryl alcohol to make a solution with a concentration of 13%, and then the ionic surfactant lauryl sulfate Sodium was dissolved in ethanol and added to the polymer solution at a final concentration of 0.48 g / L.

[0019] (3) Electrospinning and collection: put the spinning liquid into a 1ml syringe, control the flow rate to 2ml / h through a syringe pump, and the voltage is 8kv. The distance between the collection device and the tip of the syringe is 10cm, and the collection device is circular with a diameter of 5cm. , made of aluminum foil with a thickness of 100mm, the arrangement of nanofibe...

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PUM

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Abstract

The invention relates to a cardiac stent with a three-dimensional structure and anisotropy and a preparation method thereof. According to the cardiac stent, a nanometer fiber stent is weaved by static spinning, a meshed structure is defined by adjusting the orientation, density and length of spinning fibers, suspended nanometer fibers without a substrate allow the attachment and growth of cells, and an excitable mesh is formed by the nanometer fiber stent in a hollow tissue form. The cardiac stent has the advantages that: fiber materials prepared by the method can be used for a system for constructing cardiac tissues in vitro, and the anisotropy of the cells is controlled by aggregating the cells on the nanometer fiber mesh for growing; the nanometer fiber mesh is covered on a support made from polydimethylsiloxane by electric spinning to be suspended in the air; and the three-dimensional structure and the anisotropy can be kept, so that the transmission of nutrient and metabolic products is facilitated by a polymethyl glutarimide fiber-based tissue structure, and an ideal system is provided for tissue engineering. A preparation process is simple and convenient to operate, and has a bright application prospect.

Description

Technical field: [0001] The invention relates to a cardiac nanofiber scaffold and a preparation method thereof, more specifically, a cardiac scaffold with a three-dimensional structure and anisotropy and a preparation method thereof. Background technique: [0002] Tissue engineering is a rapidly growing field that builds on and fuses advances in tissue culture and materials science. Tissue engineering aims to create organ-specific tissue structures for regenerative medicine, such as implantable tissues and organs. An important feature of cardiac tissue is its highly developed structural and functional diversity, and its perfect functional coordination enables cardiomyocytes to act as a pump, which also increases the complexity of cardiac tissue engineering strategies. [0003] The cultivation of cardiomyocyte monolayers is a fundamental approach in regenerative medicine. Attempts to mimic the complexity of natural myocardium by culturing ordered monolayers of anisotropic c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61F2/82D01D1/02D01D5/00D04H1/728
Inventor 韩志超许杉杉
Owner WUXI ZHONGKE GUANGYUAN BIOMATERIALS
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