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Method for producing trivalent inactivated vaccine against Haemophilus parasuis infection
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A technology of Haemophilus suis and inactivated vaccines, applied in the field of veterinary biological products, can solve problems such as losses in pig farming
Active Publication Date: 2012-06-20
YEBIO BIOENG OF QINGDAO
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Problems solved by technology
With the development of the pig industry, Haemophilus parasuis has become a new bacterial infectious disease that seriously endangers the pig industry in recent years. It is distributed worldwide and has been confirmed to exist and prevail in most pig farms in my country. It can affect pigs aged 2 weeks to 4 months, but it mainly occurs around weaning and piglet stage. It is common in pigs aged 5-8 weeks. The incidence rate is generally 10-15%, and the mortality rate can reach more than 50% in severe cases , causing huge losses to my pig industry
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Embodiment 1
[0100] 1. Preparation of seeds for production
[0101] (1) Propagation and identification of first-level seeds. Streak the freeze-dried strains of YBH04, YBH05, and YBH13 on TSA agar plates, and inoculate them at 5%-10% CO 2 Under conditions, culture at 37°C for 24 to 48 hours, pick typical colonies or lawns, inoculate them in 100ml TSB broth, and culture at 37°C for 24 to 48 hours with shaking. Take 2ml of the bacterial liquid and pass the pure inspection, and then take it as the first-level seed. Store at -20°C for no more than 1 month; store at -70°C for no more than 3 months (see Table 13).
[0102] Table 13 First-level seeds
[0103] Strain number
Number of seeds (ml)
Use medium
Colony growth
HPS YBH04 strain
100
TSA
Round, smooth and moist, colorless and transparent colony
HPS YBH05 strain
100
TSA
Round, smooth and moist, colorless and transparent colony
HPS YBH13 strain
100
TSA
Round, smooth and moist, colorless and transparent colony
[0122] Dosage form Water-in-oil type. Take a clean straw, suck 2ml vaccine drop on the surface of cold water, it will not spread.
[0123] Stability Pipette 10ml of vaccine into a centrifuge tube, centrifuge at 3000r / min for 15min, without stratification.
[0124] Viscosity Use a 1ml pipette (the inner diameter of the lower mouth is 1.2mm, and the inner diameter of the upper mouth is 2.7mm) to suck 1.0ml of vaccine at about 25°C and allow it to flow out vertically. Record the time required to flow out 0.4ml as 4.9 seconds.
[0125] (2) Sterility test According to the method of "Chinese Veterinary Pharmacopoeia", it will grow aseptically.
[0126] (3) Safety inspection Take 5 healthy and susceptible piglets at the age of 4 to 5 weeks and inject the vaccine into the neck muscle, 4ml / head. Observation for 14 days, there is no abnormal reaction.
[0127] (4) Effectiveness test
[0128] Serological...
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Abstract
The invention relates to a method for producing a trivalent inactivated vaccine against Haemophilus parasuis infection. The method comprises the following steps of: screening Haemophilus parasuis type 4 strain YBH04, Haemophilus parasuis type 5 strain YBH05 and Haemophilus parasuis type 13 strain YBH13 from predominant prevalence strains, which have good immunogenicity and serve as vaccine strains; inoculating to appropriate culture media for culture respectively; collecting strain suspensions; inactivating with a formaldehyde solution; adding mineral oiladjuvant; and mixing and emulsifying.The vaccine is used for preventing infection of Haemophilus parasuis type 4, Haemophilus parasuis type 5 and Haemophilus parasuis type 13 in pigs, and has the advantages of good safety, high immunization efficacy and long duration of immunity.
Description
Technical field [0001] The invention relates to a method for producing a trivalent inactivated vaccine of Haemophilus parasuis, which belongs to the field of veterinary biological products. Background technique [0002] Haemophilus parasuis disease, also known as Glasser's disease, is a kind of respiratory symptoms, claudication, pleurisy, pericarditis, peritonitis, arthritis and meningitis caused by Haemophilus parasuis Respiratory tract infectious diseases characterized by pigs. With the development of the pig industry, swine parahaemophilus disease has become a new bacterial infectious disease that has seriously harmed the pig industry in recent years. It has a worldwide distribution and has been confirmed to exist and prevalent in most pig farms in my country. It can affect pigs from 2 weeks to 4 months of age, but the disease mainly occurs before and after weaning and piglets. It is common in pigs at 5-8 weeks of age. The incidence is generally 10-15%, and the mortality rate...
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