Reagent card for semiquantitatively detecting salbutamol

Abstract

The invention relates to a reagent card for semiquantitatively detecting salbutamol. The reagent card comprises a shell and a test paper strip which is arranged in the shell; the test paper strip comprises a substrate polyvinyl chloride (PVC) plate, a detection reaction part which is adhered to the central part of the substrate PVC plate, a colloidal gold pad and a water adsorption pad which are adhered to left and right ends of the detection reaction part respectively, and a sample pad which is adhered to the left end of the colloidal gold pad; and the detection reaction part is a nitrocellulose membrane; and the surface of the detection reaction part is sequentially provided with more than two detection lines coated with conjugated antigen for detecting the salbutamol, and a quality control line coated with goat anti mouse intravenous gamma globulin (IgG) from left to right, wherein the concentration of conjugated antigen is gradually reduced. The reagent card is high in specificity and accuracy, and any concentration range out of the detection limit of the reagent card for semiquantitatively detecting the salbutamol can be detected according to actual requirements. The correlation of the detection result of the reagent card, and detection results of gas chromatography-mass spectrometry (GC-MS), high performance liquid chromatography (HPLC), liquid chromatography-mass spectrometry (LC-MS) and other methods fully meet the requirements, special operation staff are not needed, and the reagent card is suitable for field detection and is high in detection speed.

Description

technical field [0001] The invention relates to a semi-quantitative detection reagent card for albuterol, which belongs to the technical field of immunology. technical background [0002] Albuterol, also known as albuterol, is a selective beta 2 receptor agonist. It can promote the growth of skeletal muscle (lean meat) and reduce fat accumulation in animals. It is often illegally used as a growth-promoting agent in the production of pigs, cattle, chickens and other livestock and poultry, which can improve feed conversion rate and increase lean meat production. However, the residue of albuterol in food can cause palpitation, headache, dizziness, dyspnea, and seriously affect people's health. In addition, the technical means to detect salbutamol residues are relatively lagging behind. Therefore, in order to seek economic benefits, some lawbreakers illegally use albuterol instead of clenbuterol as a growth-promoting agent in farms. Therefore, while controlling the illegal ...

AI Technical Summary

Problems solved by technology

However, this method still needs equipment such as a microplate reader, and the analysis time is 0.5-3 hours, which is difficult to adopt in the field

[0004] In order to further optimize the detection method and make the detection of salbutamol more convenient, shorter in time and lower in cost, relevant units have carried out research on the detection of salbutamol colloidal gold test strips, and there are "salbutamol rapid detection test strips", "β-excited The three-way test strip for the detection of salbutamol, clenbuterol hydrochloride, ractopamine and its preparation method” reported that the technology described in the report can only be used for qualitative detection, and there are only 2 combined lines, so it cannot be used for quantitative detection.

Images