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Method of nanometer tissue culture of iris ensata

A tissue culture and nanotechnology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as affecting the application and promotion of Yuchan garden forests, intensifying the contradiction between supply and demand of seedlings, and weak growth of ramet seedlings. Shorten the breeding time, improve the survival rate, and the effect of vigorous growth

Inactive Publication Date: 2014-03-05
常熟市润丰农业有限公司
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Problems solved by technology

[0004] Same as other rhizome irises, Japonica has both sexual seeds and asexual ramifications. However, the use of seeds for propagation is slow, the reproduction efficiency is low, and the rate of variation is high, while the use of ramets will cause As a result, the growth potential of ramet seedlings is getting weaker and weaker. The technology of using tissue culture technology to breed Yu Chanhua has not yet been studied, and the popularization of fine varieties has not been completed.

Method used

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Embodiment Construction

[0020] The present invention will be further described below in conjunction with the examples. The following examples are only used to illustrate the present invention, but not to limit the present invention.

[0021] The nano-tissue culture method of Yu Chan flower is characterized in that the method comprises the following steps:

[0022] (1) Selection of explants: take the stem segments and sub-nodes of the scapes as explants before the flowering of Yuchania japonica;

[0023] (2) Material processing method: first rinse the explants with sterile water, remove the leaf sheaths, cut into 3-5cm long sections with 1-4 axillary buds, and soak them in 0.5g / L carbendazim solution for 2- For 3 hours, irradiate with ultraviolet light for 20 minutes, then sterilize with 75% ethanol for 30 seconds, soak in 0.1% mercuric chloride water for 20 minutes, rinse with sterile water for 5-6 times, take out the material, cut into one axillary bud with a length of For a small section of about ...

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Abstract

The invention discloses a method of a nanometer tissue culture of iris ensata. The good effects of quick multiplication rate, stable hereditary feature, high reproduction coefficient and exuberant test-tube plantlet growth are reached by mainly adding nanometer additives in primary culture, second culture, multiplication culture and rooting culture during a tissue culture process, a germinating rate of a callus of the iris ensata is up to 98%, the multiplication multiple is up to 4.55 times, the rooting rate is up to 100%, and the transplanting survival rate is up to 98%.

Description

technical field [0001] The present invention relates to a method for tissue culture of Japonica japonica. Background technique [0002] The genus Iris is a perennial herbaceous plant belonging to the family Iridaceae, widely distributed in the northern temperate zone, with differentiation centers from China to the Middle East (Paleo-Mediterranean). China is very rich in resources of Iris, especially Iris bearded mainly distributed in the southwest, northwest and northeast regions. Iris has wide ecological adaptability, strong resistance, is suitable for cultivation in different habitats, is resistant to extensive management, and has a high reproduction coefficient. After frost in early spring and autumn, it can also be used as feed for cattle and sheep, with extremely wide application prospects. Domestic research on Iris started relatively late, and mainly focused on taxonomic research, and most species are still in a wild state that has not yet been exploited. [0003] J...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 李玉弟
Owner 常熟市润丰农业有限公司
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