Method for preparing 17beta-estradiol molecularly imprinted polymer by adopting mixed template

Abstract

The invention provides a method for preparing a 17beta-estradiol molecularly imprinted polymer by adopting a mixed template, comprising the steps of taking a mixture of 17beta-estradiol and 17alpha-estradiol as template molecules, selecting and using methacrylic acid or methyl methacrylate as functional monomers, subjecting to ultrasonic processing after mixing with a pore-forming agent, adding a cross-linking agent, standing for 1h, adding an initiator, introducing nitrogen to seal, carrying out bulk polymerization in a container by adopting a thermal initiation way, grinding and sieving the polymer, eluting the template molecule by using a Soxhlet extraction method, drying in vacuum and obtaining the 17beta-estradiol molecularly imprinted polymer. The method has the advantages that 17beta-estradiol can be high-efficiently gathered, therefore, the detection time can be shortened, the detection cost is reduced, and the method has wide application prospects.

Description

technical field

[0001] The invention belongs to the technical field of polymer materials and food safety inspection, and relates to a method for preparing 17β-estradiol molecularly imprinted polymers by using mixed templates. Background technique

[0002] 17β-estradiol is a kind of natural estrogen. After entering the human body, it will interfere with the normal endocrine system of the human body, causing excess hormones in the human body, disordering the endocrine system, and seriously affecting human health. There may be residues in the environment or in animal foods, and there is potential harm to people's health, so it has received widespread attention. At present, the techniques mainly used for the detection of 17β-estradiol are fluorescence spectrometry analysis, mass spectrometry analysis, gas chromatography, immunoassay and combined analysis techniques. However, these existing methods have certain limitations. Among them, chromatography requires expensive large-sca...

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