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Method for preparing 17beta-estradiol molecularly imprinted polymer by adopting mixed template

A technology of mixed templates and molecular imprinting, applied in chemical instruments and methods, other chemical processes, etc., can solve the problem of polymers without high affinity, achieve the effects of shortening detection time, reducing detection cost, and broad application prospects

Inactive Publication Date: 2012-07-04
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, most of the articles about 17β-estradiol molecularly imprinted polymers published by domestic and foreign scholars use a single template molecule, so the synthesized polymers do not have high affinity.

Method used

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  • Method for preparing 17beta-estradiol molecularly imprinted polymer by adopting mixed template

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] In a 100ml medical saline bottle, dissolve 0.1mmol 17α-estradiol and 0.1mmol 17β-estradiol in 3ml acetonitrile, then add 1mmol methyl methacrylate (MA) to the acetonitrile, ultrasonicate for 3 minutes and mix well Add 1mmol ethylene glycol dimethacrylate (EGDMA), sonicate for 3min to make it mix uniformly, then let it stand at room temperature for 1h, add 20mg of initiator azobisisobutyronitrile after standing, and pass N 2 , seal it quickly after 5 minutes, and react in a 50°C water bath shaker for 36 hours after sealing. After the reaction, a block polymer is formed. After the block polymer is crushed, ground, and sieved, a particle size of 30-60 μm is obtained. Particles, the particles are eluted with a mixture of 100ml methanol and acetic acid by Soxhlet extraction, and the template molecules are removed until the eluent does not contain template molecules. The volume fraction of acetic acid in the mixture is 10%, and Soxhlet extraction is performed. The conditions ...

Embodiment 2

[0024] In a 100ml medical saline bottle, dissolve 0.1mmol 17α-estradiol and 0.15mmol 17β-estradiol in 5ml acetonitrile, then add 1mmol methacrylic acid (MAA) to the acetonitrile, sonicate for 3min and mix well, then add 1mmol EGDMA, sonicate for 4min to make it evenly mixed, then stand at room temperature for 1h, add 20mg of initiator azobisisobutyronitrile after standing, and then pass through N 2 After 10 minutes, it was quickly sealed, and after sealing, it was reacted in a water bath shaker at 55°C for 32 hours. After the reaction, a block polymer was formed. After the block polymer was crushed, ground, and sieved, a particle size of 30-60 μm was obtained. Particles, the particles are eluted with a mixture of 100ml methanol and acetic acid by Soxhlet extraction, and the template molecules are removed until the eluent does not contain template molecules. The volume fraction of acetic acid in the mixture is 10%, and Soxhlet extraction is performed. The conditions were as fol...

Embodiment 3

[0026] In a 100ml medical saline bottle, dissolve 0.2mmol 17α-estradiol and 0.1mmol 17β-estradiol in 5ml chloroform, then add 1mmol MAA to the chloroform, sonicate for 3min and mix well, then add 1mmol EGDMA, sonicate for 5min Make it mix evenly, then stand at room temperature for 1h, add 25mg initiator azobisisobutyronitrile after standing, then pass through N 2 , quickly sealed after 8 minutes, and reacted in a 65°C water bath shaker for 16 hours after sealing. After the reaction, blocky polymers were formed. After the blocky polymers were crushed, ground, and sieved, particles with a particle size of 30-60 μm were obtained. Particles, the particles are eluted with a mixture of 100ml methanol and acetic acid by Soxhlet extraction, and the template molecules are removed until the eluent does not contain template molecules. The volume fraction of acetic acid in the mixture is 10%, and Soxhlet extraction is performed. The conditions were as follows: extraction for 3 times, 12 h...

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Abstract

The invention provides a method for preparing a 17beta-estradiol molecularly imprinted polymer by adopting a mixed template, comprising the steps of taking a mixture of 17beta-estradiol and 17alpha-estradiol as template molecules, selecting and using methacrylic acid or methyl methacrylate as functional monomers, subjecting to ultrasonic processing after mixing with a pore-forming agent, adding a cross-linking agent, standing for 1h, adding an initiator, introducing nitrogen to seal, carrying out bulk polymerization in a container by adopting a thermal initiation way, grinding and sieving the polymer, eluting the template molecule by using a Soxhlet extraction method, drying in vacuum and obtaining the 17beta-estradiol molecularly imprinted polymer. The method has the advantages that 17beta-estradiol can be high-efficiently gathered, therefore, the detection time can be shortened, the detection cost is reduced, and the method has wide application prospects.

Description

technical field [0001] The invention belongs to the technical field of polymer materials and food safety inspection, and relates to a method for preparing 17β-estradiol molecularly imprinted polymers by using mixed templates. Background technique [0002] 17β-estradiol is a kind of natural estrogen. After entering the human body, it will interfere with the normal endocrine system of the human body, causing excess hormones in the human body, disordering the endocrine system, and seriously affecting human health. There may be residues in the environment or in animal foods, and there is potential harm to people's health, so it has received widespread attention. At present, the techniques mainly used for the detection of 17β-estradiol are fluorescence spectrometry analysis, mass spectrometry analysis, gas chromatography, immunoassay and combined analysis techniques. However, these existing methods have certain limitations. Among them, chromatography requires expensive large-sca...

Claims

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Application Information

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IPC IPC(8): C08F220/06C08F220/14C08F222/14C08J9/28C08J9/26B01J20/26B01J20/30
Inventor 龚国利贾琳李慧陈松
Owner SHAANXI UNIV OF SCI & TECH
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