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Method for preparing 17beta-estradiol molecularly imprinted polymer by adopting mixed template

A technology of mixed templates and molecular imprinting, applied in chemical instruments and methods, other chemical processes, etc., can solve the problem of polymers without high affinity, achieve the effects of shortening detection time, reducing detection cost, and broad application prospects

Inactive Publication Date: 2013-11-27
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the articles about 17β-estradiol molecularly imprinted polymers published by domestic and foreign scholars use a single template molecule, so the synthesized polymers do not have high affinity.

Method used

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  • Method for preparing 17beta-estradiol molecularly imprinted polymer by adopting mixed template

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] In a 100ml medical saline bottle, dissolve 0.1mmol of 17α-estradiol and 0.1mmol of 17β-estradiol in 3ml of acetonitrile, then add 1mmol of methyl methacrylate (MA) to the acetonitrile, ultrasonically treat for 3min and mix well Add 1 mmol of ethylene glycol dimethacrylate (EGDMA), ultrasonically treat it for 3 min to make it evenly mixed, then let stand for 1 h at room temperature, add 20 mg of initiator azobisisobutyronitrile after standing, and then pass N 2 After 5 minutes, it was quickly sealed. After sealing, it was reacted in a water bath shaker at 50 °C for 36 hours. After the reaction was completed, a blocky polymer was generated. The particles are eluted with a mixture of 100 ml methanol and acetic acid by Soxhlet extraction, and the template molecules are removed until the eluent does not contain template molecules, and the volume fraction of acetic acid in the mixture is 10%. Soxhlet extraction The conditions of extraction were 5 times of extraction for 8 hou...

Embodiment 2

[0024] In a 100ml medical saline bottle, dissolve 0.1mmol of 17α-estradiol and 0.15mmol of 17β-estradiol in 5ml of acetonitrile, add 1mmol of methacrylic acid (MAA) to the acetonitrile, and then add 1mmol of 1mmol after sonicating for 3min and mixing evenly EGDMA, ultrasonically treated for 4 min to make it evenly mixed, then allowed to stand at room temperature for 1 h, after standing, add 20 mg of initiator azobisisobutyronitrile, and then pass N 2 After 10 minutes, it was quickly sealed. After sealing, it was reacted in a water bath shaker at 55°C for 32 hours. After the reaction was completed, a blocky polymer was generated. The particles are eluted with a mixture of 100 ml methanol and acetic acid by Soxhlet extraction, and the template molecules are removed until the eluent does not contain template molecules, and the volume fraction of acetic acid in the mixture is 10%. Soxhlet extraction The conditions of extraction were 4 times of extraction for 10 hours each time, an...

Embodiment 3

[0026] In a 100ml medical saline bottle, dissolve 0.2mmol of 17α-estradiol and 0.1mmol of 17β-estradiol in 5ml of chloroform, then add 1mmol of MAA to the chloroform, ultrasonically treat for 3min and mix well, add 1mmol of EGDMA, and ultrasonically treat for 5min Make it evenly mixed, then stand at room temperature for 1h, add 25mg initiator azobisisobutyronitrile after standing, and then pass N 2 After 8 minutes, it was quickly sealed. After sealing, it was reacted in a water bath shaker at 65°C for 16 hours. After the reaction was completed, a blocky polymer was generated. The particles are eluted with a mixture of 100 ml methanol and acetic acid by Soxhlet extraction, and the template molecules are removed until the eluent does not contain template molecules, and the volume fraction of acetic acid in the mixture is 10%. Soxhlet extraction The conditions of extraction were 3 times of extraction for 12 hours each time, and finally eluted with methanol twice to remove acetic ...

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Abstract

The invention provides a method for preparing a 17beta-estradiol molecularly imprinted polymer by adopting a mixed template, comprising the steps of taking a mixture of 17beta-estradiol and 17alpha-estradiol as template molecules, selecting and using methacrylic acid or methyl methacrylate as functional monomers, subjecting to ultrasonic processing after mixing with a pore-forming agent, adding a cross-linking agent, standing for 1h, adding an initiator, introducing nitrogen to seal, carrying out bulk polymerization in a container by adopting a thermal initiation way, grinding and sieving the polymer, eluting the template molecule by using a Soxhlet extraction method, drying in vacuum and obtaining the 17beta-estradiol molecularly imprinted polymer. The method has the advantages that 17beta-estradiol can be high-efficiently gathered, therefore, the detection time can be shortened, the detection cost is reduced, and the method has wide application prospects.

Description

technical field [0001] The invention belongs to the technical field of polymer materials and food safety inspection, and relates to a method for preparing a 17β-estradiol molecularly imprinted polymer by using a mixed template. Background technique [0002] 17β-estradiol is a natural estrogen. After entering the human body, it will interfere with the normal endocrine system of the human body, resulting in excess hormones in the human body, disorder of the endocrine system, and serious impact on human health. There may be residues in the environment or in animal foods, which have potential harm to people's health, so they have received widespread attention. At present, the main techniques used for the detection of 17β-estradiol are fluorescence spectroscopy, mass spectrometry, gas chromatography, immunoassay and combined analytical techniques. However, these existing methods have certain limitations. Among them, chromatography requires expensive large-scale instruments and p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F220/06C08F220/14C08F222/14C08J9/28C08J9/26B01J20/26B01J20/30
Inventor 龚国利贾琳李慧陈松
Owner SHAANXI UNIV OF SCI & TECH
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