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Quality control method of sugar-free erigeron breviscapine mixture

A quality control method, the technology of Erigeron breviscapus, is applied in the field of quality control of sugar-free Erigeron breviscapus mixture, which can solve the problems of easy introduction of impurities, influence on separation, and difficulty in separating the upper liquid, so as to achieve good separation effect and easy operation , fast and specific effects

Inactive Publication Date: 2012-07-11
昆明振华制药厂有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the existing quality standard of "Erigeron breviscens Mixture" (YBZ00612004): 1. In the "Identification" item, it must be noted that the temperature of the developing agent should be higher than 5°C, otherwise the upper liquid will be cloudy and difficult to separate; 2. The thin layer of caffeic acid For chromatographic identification, it is necessary to pay attention to the separation time of n-butanol extraction, otherwise it is easy to introduce impurities and affect the separation

Method used

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  • Quality control method of sugar-free erigeron breviscapine mixture
  • Quality control method of sugar-free erigeron breviscapine mixture
  • Quality control method of sugar-free erigeron breviscapine mixture

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Embodiment 1

[0025] 1. The present invention identifies the medicinal ingredients and caffeic acid in the sugar-free scutellaria scutellaria mixture as follows:

[0026] 1) Identification of medicinal ingredients of Erigeron breviscapus: Take 4ml of this product, add hydrochloric acid to adjust the pH value to about 2, put it in a separatory funnel, add 10ml of ethyl acetate, shake and extract, separate the ethyl acetate layer, and concentrate in a water bath to 1ml, as the test solution; take another 2g of Erigeron breviscapitalis reference medicinal material, add 50ml of water, heat and reflux on a water bath for 1 hour, filter while hot, concentrate the filtrate to 10ml, add 20ml of ethanol, stir well, and place in the refrigerator overnight. Filter, put the filtrate on a water bath to evaporate the ethanol, add an appropriate amount of water to make it 10ml, adjust the pH to 7 with sodium hydroxide test solution, add 0.2g of activated carbon, boil for 5 minutes, filter, add hydrochloric...

Embodiment 2

[0043] 1. The present invention identifies the medicinal ingredients and caffeic acid in the sugar-free scutellaria scutellaria mixture as follows:

[0044] 1) Identification of medicinal ingredients of Erigeron breviscapine: Take 4ml of this product, add hydrochloric acid to adjust the pH value to about 2, put it in a separatory funnel, add 20ml of ethyl acetate, shake and extract, separate the ethyl acetate layer, and concentrate it in a water bath to 1ml, as the test solution; take another 2g of Erigeron breviscapus reference medicinal material, add 50ml of water, heat and reflux on a water bath for 1 hour, filter while hot, concentrate the filtrate to 20ml, add 20ml of ethanol, stir well, and place in the refrigerator overnight. Filter, put the filtrate on a water bath to evaporate ethanol, add appropriate amount of water to make 20ml, adjust the pH to 9 with sodium hydroxide test solution, add 0.2g of activated carbon, boil for 5 minutes, filter, add hydrochloric acid to t...

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Abstract

The invention discloses a quality control method of a sugar-free erigeron breviscapine mixture. The quality control method comprises the following steps of: 1) identifying a breviscapine medicinal component from caffeic acid in the sugar-free erigeron breviscapine mixture: (1) identifying the breviscapine medicinal component, and (2) identifying breviscapine caffeic acid; and 2) measuring the content of scutellarin in the sugar-free erigeron breviscapine mixture: (1) testing chromatographic conditions and system adaptability, (2) preparing a control product solution, (3) preparing a sample solution, and (4) testing with a testing method. The method adopted in the invention is used for identifying and checking Chinese medicinal components including breviscapine and caffeic acid in the sugar-free erigeron breviscapine mixture with a thin layer chromatography. The method has the technical characteristics of easiness and convenience for operating, good separating effect, high specificity and the like; the content of scutellarin serving as a major active ingredient in a prescription is measured with a high performance liquid chromatography (HPLC); and the method has the characteristics of good separating effect, accurate, sensitive and quick measurement, high specificity, and the like.

Description

[0001] technical field [0002] The invention relates to the technical field of quality control of medicines, in particular to a quality control method of sugar-free breviscapine mixture. Background technique [0003] Asarum breviscapus extract is the main active ingredient, used for hemiplegia caused by blood stasis syndrome cerebral infarction, rheumatic pain, angina pectoris of coronary heart disease, fundus retinal vein occlusion, vasculitic skin diseases, etc. It contains 70mg of total flavonoids and not less than 4.0mg of scutellarin. In the existing quality standard of "Erigeron breviscens Mixture" (YBZ00612004): 1. In the "Identification" item, it must be noted that the temperature of the developing agent should be higher than 5°C, otherwise the upper liquid will be cloudy and difficult to separate; 2. The thin layer of caffeic acid For chromatographic identification, it should be noted that the separation time should be sufficient during n-butanol extraction, other...

Claims

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Application Information

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IPC IPC(8): A61K36/28A61K9/00G01N30/02G01N30/90A61P9/10A61P25/02A61P29/00A61P27/02A61P9/00A61P17/00
Inventor 李俊罗珊珊
Owner 昆明振华制药厂有限公司
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